Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSEBR_RS25785 Genome accession   NC_015379
Coordinates   5852661..5853878 (+) Length   405 a.a.
NCBI ID   WP_003205670.1    Uniprot ID   A0AAD1GU29
Organism   Pseudomonas brassicacearum subsp. brassicacearum NFM421     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5847661..5858878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEBR_RS25765 (PSEBR_a4823) - 5849057..5849353 (+) 297 WP_013694228.1 DUF2845 domain-containing protein -
  PSEBR_RS25770 (PSEBR_a4824) - 5849682..5850035 (-) 354 WP_013694229.1 BON domain-containing protein -
  PSEBR_RS25775 (PSEBR_a4825) pilA 5850308..5850733 (-) 426 WP_013694230.1 pilin Machinery gene
  PSEBR_RS25780 (PSEBR_a4826) pilB 5850958..5852658 (+) 1701 WP_013694231.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSEBR_RS25785 (PSEBR_a4827) pilC 5852661..5853878 (+) 1218 WP_003205670.1 type II secretion system F family protein Machinery gene
  PSEBR_RS25790 (PSEBR_a4828) pilD 5853880..5854749 (+) 870 WP_013694232.1 prepilin peptidase Machinery gene
  PSEBR_RS25795 (PSEBR_a4829) coaE 5854749..5855372 (+) 624 WP_013694233.1 dephospho-CoA kinase -
  PSEBR_RS25800 (PSEBR_a4830) yacG 5855369..5855575 (+) 207 WP_003205675.1 DNA gyrase inhibitor YacG -
  PSEBR_RS25805 (PSEBR_a4831) - 5855572..5855787 (-) 216 WP_013694234.1 hypothetical protein -
  PSEBR_RS25810 (PSEBR_a4832) - 5855851..5856537 (-) 687 WP_003205678.1 energy-coupling factor ABC transporter permease -
  PSEBR_RS25815 (PSEBR_a4833) - 5856579..5856989 (-) 411 WP_003205679.1 tautomerase family protein -
  PSEBR_RS25820 (PSEBR_a4834) - 5857142..5858035 (+) 894 WP_003205681.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44246.00 Da        Isoelectric Point: 10.0786

>NTDB_id=40277 PSEBR_RS25785 WP_003205670.1 5852661..5853878(+) (pilC) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MAVKAVKTDVYTWEGKDRKGTKMSGELTGQSPALVKAQLRKQGINPQKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAKLPAFTVMVIGLSEIMQQWWWVMLGGAVGAFFGVK
YALRRSERFRDWRDKWLLKLPLIGTLLYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIKQEVSTGM
QLNFSMRASGIFPNLAVQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=40277 PSEBR_RS25785 WP_003205670.1 5852661..5853878(+) (pilC) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGGCGGTCAAGGCAGTCAAAACCGATGTTTACACTTGGGAAGGCAAGGACCGCAAAGGCACGAAAATGAGTGGCGAGCT
GACGGGTCAAAGCCCGGCGCTGGTCAAGGCACAGCTGCGCAAACAAGGCATCAACCCGCAGAAGGTGCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGATATCGCCCTCTTCACCCGCCAGATGGCCACCATGCTCAAG
GCTGGTGTGCCGCTGTTGCAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACCCCAACATGCGCAAGCTGGTGGACGA
GGTGAAGCAGGAAGTCGCCGCTGGCAACAGCTTCGCCGCGGCGCTGCGCAAGTGCCCGCAATATTTCGACGAGCTGTACT
GCAACCTGGTAGACGCCGGCGAGCAGGCCGGTGCCCTGGACACGCTGCTGGACCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCAGCGGCAGTGGTCCTCGTCGCCGCAGTGGTCACGGGGAT
CCTGCTGGTCAAGGTCGTGCCGCAATTCGAATCGGTATTCTCCGGGTTTGGCGCGAAACTGCCAGCCTTCACAGTGATGG
TCATAGGCCTGTCGGAAATCATGCAGCAATGGTGGTGGGTGATGCTTGGCGGGGCGGTGGGGGCTTTTTTTGGCGTGAAG
TATGCGCTCAGGCGTTCGGAGCGCTTTCGCGACTGGCGCGACAAATGGCTGCTCAAGCTGCCGCTGATCGGCACCTTGCT
GTACAAATCCGCCGTGGCCCGCTACGCCCGCACGCTGTCCACTACCTTCGCCGCCGGCGTGCCGCTGGTGGAGGCCCTGG
ATTCGGTGTCTGGCGCCACCGGCAACGTGGTATTCAAACGTGCCGTGCAGCGCATCAAGCAGGAGGTCTCGACCGGCATG
CAGTTGAATTTCTCCATGCGTGCCTCAGGGATTTTTCCGAACCTGGCGGTCCAGATGACCGCCATCGGCGAGGAATCCGG
TGCGCTGGACGACATGCTCGACAAGGTGGCGAGCTTTTATGAGGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTGGTCGTCGGTGGCCTGGTAGTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.556

100

0.756
  pilC Acinetobacter baylyi ADP1

58.853

99.012

0.583
  pilC Acinetobacter baumannii D1279779

57.598

100

0.58
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilC Vibrio campbellii strain DS40M4

44.192

97.778

0.432
  pilG Neisseria meningitidis 44/76-A

42.469

100

0.425
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilG Neisseria gonorrhoeae MS11

42.537

99.259

0.422
  pilC Thermus thermophilus HB27

37.406

99.012

0.37

Multiple sequence alignment