Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PSEBR_RS25775 Genome accession   NC_015379
Coordinates   5850308..5850733 (-) Length   141 a.a.
NCBI ID   WP_013694230.1    Uniprot ID   A0AAD1GWQ3
Organism   Pseudomonas brassicacearum subsp. brassicacearum NFM421     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5845308..5855733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEBR_RS25760 (PSEBR_a4822) - 5846122..5849055 (+) 2934 WP_013694227.1 DUF748 domain-containing protein -
  PSEBR_RS25765 (PSEBR_a4823) - 5849057..5849353 (+) 297 WP_013694228.1 DUF2845 domain-containing protein -
  PSEBR_RS25770 (PSEBR_a4824) - 5849682..5850035 (-) 354 WP_013694229.1 BON domain-containing protein -
  PSEBR_RS25775 (PSEBR_a4825) pilA 5850308..5850733 (-) 426 WP_013694230.1 pilin Machinery gene
  PSEBR_RS25780 (PSEBR_a4826) pilB 5850958..5852658 (+) 1701 WP_013694231.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSEBR_RS25785 (PSEBR_a4827) pilC 5852661..5853878 (+) 1218 WP_003205670.1 type II secretion system F family protein Machinery gene
  PSEBR_RS25790 (PSEBR_a4828) pilD 5853880..5854749 (+) 870 WP_013694232.1 prepilin peptidase Machinery gene
  PSEBR_RS25795 (PSEBR_a4829) coaE 5854749..5855372 (+) 624 WP_013694233.1 dephospho-CoA kinase -
  PSEBR_RS25800 (PSEBR_a4830) yacG 5855369..5855575 (+) 207 WP_003205675.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14175.29 Da        Isoelectric Point: 7.5917

>NTDB_id=40275 PSEBR_RS25775 WP_013694230.1 5850308..5850733(-) (pilA) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MNKQSGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLAEVSALKVPFEDIMNQGTNPTLANIGGTSPTSNCTLT
ASGTAADGAGTIACTLLNAPAPVLGKAITLTRSTQGVWTCGSTAAQEFLPAGCTGTAATTP

Nucleotide


Download         Length: 426 bp        

>NTDB_id=40275 PSEBR_RS25775 WP_013694230.1 5850308..5850733(-) (pilA) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGAATAAGCAGAGTGGCTTTACCTTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGTATTCTGGCGACGTTTGCGTT
GCCGCAATATTCGAAGTATCAGGCGCGGGCCAAGGCCACGGCGGGGCTGGCGGAGGTCTCTGCGTTGAAAGTGCCGTTTG
AGGACATCATGAATCAAGGTACCAATCCAACGTTGGCCAACATCGGCGGGACCTCGCCCACCAGCAATTGCACCTTGACG
GCTTCAGGAACAGCAGCGGACGGAGCGGGCACCATCGCGTGTACCCTTTTGAATGCACCCGCTCCAGTGCTTGGCAAAGC
GATCACCCTGACGCGTTCTACTCAAGGCGTTTGGACCTGCGGTTCGACCGCCGCTCAGGAATTCCTGCCGGCTGGATGCA
CAGGTACAGCAGCGACGACTCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50.725

97.872

0.496
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.715

97.163

0.454
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.284

95.035

0.411
  pilA Pseudomonas aeruginosa PAK

38.926

100

0.411
  comP Acinetobacter baylyi ADP1

37.748

100

0.404
  pilA2 Legionella pneumophila strain ERS1305867

39.855

97.872

0.39
  pilA2 Legionella pneumophila str. Paris

39.855

97.872

0.39
  pilA Ralstonia pseudosolanacearum GMI1000

33.54

100

0.383
  pilA Vibrio cholerae C6706

37.063

100

0.376
  pilA Vibrio cholerae strain A1552

37.063

100

0.376
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.063

100

0.376
  pilA Vibrio parahaemolyticus RIMD 2210633

39.844

90.78

0.362

Multiple sequence alignment