Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSEBR_RS25780 Genome accession   NC_015379
Coordinates   5850958..5852658 (+) Length   566 a.a.
NCBI ID   WP_013694231.1    Uniprot ID   A0AAD1GVA0
Organism   Pseudomonas brassicacearum subsp. brassicacearum NFM421     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5845958..5857658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEBR_RS25760 (PSEBR_a4822) - 5846122..5849055 (+) 2934 WP_013694227.1 DUF748 domain-containing protein -
  PSEBR_RS25765 (PSEBR_a4823) - 5849057..5849353 (+) 297 WP_013694228.1 DUF2845 domain-containing protein -
  PSEBR_RS25770 (PSEBR_a4824) - 5849682..5850035 (-) 354 WP_013694229.1 BON domain-containing protein -
  PSEBR_RS25775 (PSEBR_a4825) pilA 5850308..5850733 (-) 426 WP_013694230.1 pilin Machinery gene
  PSEBR_RS25780 (PSEBR_a4826) pilB 5850958..5852658 (+) 1701 WP_013694231.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSEBR_RS25785 (PSEBR_a4827) pilC 5852661..5853878 (+) 1218 WP_003205670.1 type II secretion system F family protein Machinery gene
  PSEBR_RS25790 (PSEBR_a4828) pilD 5853880..5854749 (+) 870 WP_013694232.1 prepilin peptidase Machinery gene
  PSEBR_RS25795 (PSEBR_a4829) coaE 5854749..5855372 (+) 624 WP_013694233.1 dephospho-CoA kinase -
  PSEBR_RS25800 (PSEBR_a4830) yacG 5855369..5855575 (+) 207 WP_003205675.1 DNA gyrase inhibitor YacG -
  PSEBR_RS25805 (PSEBR_a4831) - 5855572..5855787 (-) 216 WP_013694234.1 hypothetical protein -
  PSEBR_RS25810 (PSEBR_a4832) - 5855851..5856537 (-) 687 WP_003205678.1 energy-coupling factor ABC transporter permease -
  PSEBR_RS25815 (PSEBR_a4833) - 5856579..5856989 (-) 411 WP_003205679.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62763.76 Da        Isoelectric Point: 5.4307

>NTDB_id=40276 PSEBR_RS25780 WP_013694231.1 5850958..5852658(+) (pilB) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MNDIALSGLTKQLVLAELLTEQSAHQAYQQAQRNRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTRQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGILREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCSHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNNGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=40276 PSEBR_RS25780 WP_013694231.1 5850958..5852658(+) (pilB) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCACCAGGC
GTATCAACAGGCGCAACGCAATCGCATCCCCCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCCAGAGCCGACAGGTCG
CGGAGATCGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAAACCCAACCCACCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTGGCGGCGCGGCAACAAGCTGTACGTGGGCATCTCCGA
CCCGACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTTTTCGAATCCAGCAGCACGGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAATCGATCGACGACACCAGGCAGGACTCCATCGCCGGCCAGGACACCGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAAATCTACCGGG
TGCGGGTGCGCACCGACGGCATACTGCGGGAAGTGGCCAGGCCGCCGATCCAGTTGGCCAACCGTATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CACAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCGCAAGGG
CTGATCCTGGTGACTGGCCCAACCGGCTCGGGCAAGACCGTGTCGCTCTACACCGGACTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCGCGCCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAGAACATGGGCATTCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATTGCCCAGCGGC
TGGCGCGCAAGCTGTGCAGTCACTGCAAGAAGGCCATCGAGATTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCTCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAACGGTTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCACCTCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.581
  pilB Acinetobacter baumannii D1279779

57.672

100

0.578
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481
  pilF Thermus thermophilus HB27

36.042

100

0.36

Multiple sequence alignment