Detailed information NTG0040269

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSEBR_RS10470
• Genome accession: NC_015379
• Coordinates: 2401303..2402421 (+)
• Length: 372 a.a.
• NCBI ID: WP_003200243.1
• Uniprot ID: F2KEW7
• Organism: Pseudomonas brassicacearum subsp. brassicacearum NFM421
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2396303..2407421

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSEBR_RS10455 (PSEBR_a1965)	-	2397237..2398154 (+)	918	WP_013692716.1	sugar kinase	-
PSEBR_RS10460 (PSEBR_a1967)	-	2398309..2398584 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
PSEBR_RS10465 (PSEBR_a1968)	-	2398633..2401170 (-)	2538	WP_013692717.1	hybrid sensor histidine kinase/response regulator	-
PSEBR_RS10470 (PSEBR_a1969)	pilU	2401303..2402421 (+)	1119	WP_003200243.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSEBR_RS10475 (PSEBR_a1970)	-	2402613..2404568 (-)	1956	WP_003200245.1	acetoacetate--CoA ligase	-
PSEBR_RS10480 (PSEBR_a1971)	-	2404727..2405503 (-)	777	WP_013692718.1	3-hydroxybutyrate dehydrogenase	-
PSEBR_RS10485 (PSEBR_a1972)	-	2405511..2406902 (-)	1392	WP_003200250.1	GntP family permease	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41328.27 Da
• Isoelectric Point: 6.2516

>NTDB_id=40269 PSEBR_RS10470 WP_003200243.1 2401303..2402421(+) (pilU) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVIKPLGNEAFKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVVEGAIDEEEALKNADSANNLRLRLKLHSESGAAAPPADPAAGEWGLVD

Nucleotide

• Length: 1119 bp

>NTDB_id=40269 PSEBR_RS10470 WP_003200243.1 2401303..2402421(+) (pilU) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTTTACATGTCCACGGGTGCGCCACCGTG
CGCGCGTTTCGAGGGCGTGATCAAGCCCTTGGGGAACGAGGCGTTCAAGGTCGGTGACATTGCCAGGCTCGCCGAGTCTC
TCATGGACGCCGAACAGCGTCTTGAGTTCGATCGGGAGTTGGAAATGAACCTGGCGATCTCCCTGGCTGGTGTCGGGCGG
TTCCGGGTCAATATCTTCAAGCAGCGCAATGACGTGTCCATGGTGATACGCAACGTCAAGCTGGACATTCCGCGCTTCGA
AGACCTGAAACTGCCTCGGGTGCTTTTGGACACCATCATGCAGAAACAGGGGTTGATGCTGTTCGTCGGCGCCACAGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAATCAACGGGAAGTCGGTGTCGACACCCGCAGTTTCCATGC
AGCCCTGAAAAACACCCTGCGCCAGGCGCCTGACGTGGTGCTGATCGGCGAAATTCGCGACCGGGAAACCATGGAACATG
CCCTGGCGTTTGCCGACACCGGGCATCTGGTGATTTCCACCTTGCATGCCCATAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTCTTTCCGGAAGAGCGTCGGCCGCAGTTGCTCAATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTGCGCACCCGCACCGGCCAGCGGCGAGCGGCGGTGGAGGTCATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GGCGCAACGAGCTCGGCGAGCTCAAGGGCATCATGGAAAAGTCAGAGGAACTGGGGATGCAAACCTTCGATCAGGCGCTG
TTCAACCTGGTGGTCGAAGGCGCCATCGATGAGGAAGAAGCGCTGAAAAATGCCGACTCGGCGAACAACCTGCGGTTGCG
TTTGAAGTTGCATAGCGAGTCGGGGGCGGCGGCGCCACCGGCCGATCCGGCGGCGGGAGAGTGGGGGCTGGTGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-271)

3D structure

• AlphaFold DB: F2KEW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.183	95.43	0.546
pilU	Acinetobacter baylyi ADP1	52.247	95.699	0.5
pilU	Vibrio cholerae strain A1552	52.857	94.086	0.497
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.525	98.387	0.379
pilT	Vibrio cholerae strain A1552	41.141	89.516	0.368
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.516	0.368
pilT	Pseudomonas stutzeri DSM 10701	39.244	92.473	0.363
pilT	Acinetobacter nosocomialis M2	40.12	89.785	0.36
pilT	Acinetobacter baumannii D1279779	40.12	89.785	0.36
pilT	Acinetobacter baumannii strain A118	40.12	89.785	0.36
pilT	Pseudomonas aeruginosa PAK	38.953	92.473	0.36