NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	HUF04_RS27510	Genome accession	NZ_CP054581
Coordinates	5792308..5792757 (-)	Length	149 a.a.
NCBI ID	WP_003112842.1	Uniprot ID	P04739
Organism	Pseudomonas aeruginosa strain YTSEY8
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5787308..5797757

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUF04_RS27490 (HUF04_27530)	ampD	5788000..5788566 (-)	567	WP_173834560.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
HUF04_RS27495 (HUF04_27535)	-	5788717..5790972 (-)	2256	WP_003104913.1	DUF1631 domain-containing protein	-
HUF04_RS27500 (HUF04_27540)	nadC	5791258..5792106 (+)	849	WP_003116246.1	carboxylating nicotinate-nucleotide diphosphorylase	-
HUF04_RS27510 (HUF04_27550)	pilA	5792308..5792757 (-)	450	WP_003112842.1	type 4a pilus biogenesis protein PilA	Machinery gene
HUF04_RS27515 (HUF04_27555)	pilB	5792989..5794689 (+)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HUF04_RS27520 (HUF04_27560)	pilC	5794693..5795913 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
HUF04_RS27525 (HUF04_27565)	pilD	5795917..5796789 (+)	873	WP_034040535.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HUF04_RS27530 (HUF04_27570)	coaE	5796786..5797397 (+)	612	WP_034040533.1	dephospho-CoA kinase	-
HUF04_RS27535 (HUF04_27575)	yacG	5797394..5797594 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 149 a.a. Molecular weight: 15511.78 Da Isoelectric Point: 6.2905

                                >NTDB_id=400294 HUF04_RS27510 WP_003112842.1 5792308..5792757(-) (pilA) [Pseudomonas aeruginosa strain YTSEY8]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV

GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide

Download Length: 450 bp

                                >NTDB_id=400294 HUF04_RS27510 WP_003112842.1 5792308..5792757(-) (pilA) [Pseudomonas aeruginosa strain YTSEY8]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT

TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC

GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGTGGTGCGGGTGATATTACCTTTAC

CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGGCTT

GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
PDB	1PAN
PDB	1PAO
PDB	8TUM
PDB	8TUW
PDB	9EWX

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	67.785	100	0.678
pilA	Acinetobacter baumannii strain A118	48	100	0.483
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	48.322	100	0.483
pilA	Vibrio cholerae strain A1552	48.322	100	0.483
pilA	Vibrio cholerae C6706	48.322	100	0.483
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.638	100	0.456
pilA	Vibrio parahaemolyticus RIMD 2210633	44.898	98.658	0.443
comP	Acinetobacter baylyi ADP1	41.83	100	0.43
pilA/pilAII	Pseudomonas stutzeri DSM 10701	44.056	95.973	0.423
pilA2	Legionella pneumophila str. Paris	38.776	98.658	0.383
pilA2	Legionella pneumophila strain ERS1305867	38.095	98.658	0.376

Detailed information NTG0400294