Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FIU82_RS12730 Genome accession   NZ_CP045418
Coordinates   2740390..2741619 (+) Length   409 a.a.
NCBI ID   WP_152441222.1    Uniprot ID   A0A5P9J2U4
Organism   Pseudoalteromonas sp. THAF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2735390..2746619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU82_RS12710 (FIU82_12890) trmL 2736398..2736862 (+) 465 WP_152441218.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  FIU82_RS12715 (FIU82_12895) nadC 2736911..2737762 (+) 852 WP_152441219.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FIU82_RS12720 (FIU82_12900) - 2738140..2738556 (+) 417 WP_152441220.1 pilin -
  FIU82_RS12725 (FIU82_12905) pilB 2738619..2740310 (+) 1692 WP_152441221.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU82_RS12730 (FIU82_12910) pilC 2740390..2741619 (+) 1230 WP_152441222.1 type II secretion system F family protein Machinery gene
  FIU82_RS12735 (FIU82_12915) pilD 2741620..2742516 (+) 897 WP_172972730.1 A24 family peptidase Machinery gene
  FIU82_RS12740 (FIU82_12920) coaE 2742524..2743129 (+) 606 WP_152441224.1 dephospho-CoA kinase -
  FIU82_RS12745 (FIU82_12925) pilB 2743218..2744897 (+) 1680 WP_152441225.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU82_RS12750 (FIU82_12930) yacG 2744975..2745214 (+) 240 WP_152441226.1 DNA gyrase inhibitor YacG -
  FIU82_RS12755 (FIU82_12935) - 2745223..2745996 (-) 774 WP_045979345.1 YggN family protein -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44434.22 Da        Isoelectric Point: 9.4355

>NTDB_id=393911 FIU82_RS12730 WP_152441222.1 2740390..2741619(+) (pilC) [Pseudoalteromonas sp. THAF3]
MAKQKQANQLDTFQWVGVSTRGKKLEGELTGTSIALVKAQLRKQGITPSKVKRKAKPLLGFSGEKRITAKDIAMLTRQIA
TMLLAGVSLVQAIDMIAEGSENKSLKNLLRTIGEEVKAGQPLSAALKKHPKYFDDLYCDLVASGEQSGALDRIFDRVALY
KEKAEALKSKIKKAMFYPVAVIAVAIIVTSILLIFVVPQFQDIFNGFGAELPAFTLFVIGISEFMQEYWWVAVLTMVVGG
YLFKQAHRKNKDLRDNVDKLILKTPIVGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRDAILDIKAEV
SSGNPMNWAMRNSNIFPDMVIQMVAIGEESGSLDDMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLIGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=393911 FIU82_RS12730 WP_152441222.1 2740390..2741619(+) (pilC) [Pseudoalteromonas sp. THAF3]
ATGGCAAAACAAAAACAAGCAAACCAATTGGACACGTTCCAATGGGTGGGTGTGAGTACTCGAGGCAAAAAACTTGAAGG
TGAGCTTACTGGTACCAGTATTGCACTCGTAAAGGCTCAACTGCGCAAACAAGGTATTACGCCATCAAAGGTGAAAAGAA
AAGCCAAACCTTTACTGGGCTTTTCTGGTGAGAAGCGTATAACAGCGAAAGACATAGCCATGCTGACCCGCCAGATAGCA
ACCATGCTGCTTGCTGGTGTTTCCTTGGTTCAAGCTATAGACATGATTGCCGAAGGCTCTGAAAACAAAAGCCTAAAGAA
CCTATTAAGAACAATTGGTGAAGAAGTAAAAGCGGGTCAACCTCTGAGTGCTGCACTGAAAAAACACCCCAAGTACTTTG
ATGACCTCTACTGTGACTTGGTTGCATCAGGTGAGCAGTCAGGCGCGTTAGATAGAATATTTGACCGTGTCGCTTTATAC
AAAGAAAAAGCAGAAGCCCTCAAATCAAAAATTAAAAAGGCGATGTTTTATCCGGTGGCAGTTATAGCCGTTGCCATAAT
AGTTACATCCATTTTGCTTATCTTCGTAGTACCTCAATTCCAAGATATTTTTAATGGCTTTGGCGCAGAACTACCTGCAT
TTACCTTATTCGTAATCGGAATTTCTGAGTTTATGCAGGAGTATTGGTGGGTTGCAGTGCTAACCATGGTTGTTGGTGGT
TATTTGTTTAAGCAGGCACACCGAAAGAATAAAGATTTGCGAGACAATGTTGATAAGCTCATCCTAAAAACCCCAATCGT
AGGTATGATCTTAAATAAGGCTGCAGTAGCACGTTATGCCCGAACATTATCGACCACTTTTGCCGCAGGTGTCCCTTTAG
TGGATGCACTAGATTCAGCAGCAGGAGCCTCTGGTAATGCGGTTTATCGCGATGCAATTTTAGATATCAAAGCTGAGGTC
AGCTCTGGTAACCCGATGAACTGGGCAATGCGTAACTCAAATATATTCCCTGATATGGTGATTCAAATGGTCGCTATCGG
CGAAGAGTCTGGTTCACTGGATGATATGCTTGCTAAGGTGGCAACTATTTATGAACAAGAAGTAGATGATGCCGTGGACG
GCCTCTCAAGTCTATTAGAGCCACTCATTATGGCAGTGCTAGGGGTGCTAATCGGTGGCCTAATTGTTGCTATGTATTTA
CCTATTTTCCAACTCGGTTCTGTTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9J2U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.461

97.066

0.597
  pilC Acinetobacter baumannii D1279779

54.568

99.022

0.54
  pilC Acinetobacter baylyi ADP1

54.054

99.511

0.538
  pilC Legionella pneumophila strain ERS1305867

53.563

99.511

0.533
  pilC Vibrio cholerae strain A1552

44.878

100

0.45
  pilC Vibrio campbellii strain DS40M4

42.647

99.756

0.425
  pilG Neisseria meningitidis 44/76-A

43.99

95.599

0.421
  pilG Neisseria gonorrhoeae MS11

43.734

95.599

0.418
  pilC Thermus thermophilus HB27

36.881

98.778

0.364

Multiple sequence alignment