Detailed information NTG0393912

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FIU82_RS12735
• Genome accession: NZ_CP045418
• Coordinates: 2741620..2742516 (+)
• Length: 298 a.a.
• NCBI ID: WP_172972730.1
• Uniprot ID: A0A5P9IZ28
• Organism: Pseudoalteromonas sp. THAF3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2736620..2747516

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIU82_RS12715 (FIU82_12895)	nadC	2736911..2737762 (+)	852	WP_152441219.1	carboxylating nicotinate-nucleotide diphosphorylase	-
FIU82_RS12720 (FIU82_12900)	-	2738140..2738556 (+)	417	WP_152441220.1	pilin	-
FIU82_RS12725 (FIU82_12905)	pilB	2738619..2740310 (+)	1692	WP_152441221.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FIU82_RS12730 (FIU82_12910)	pilC	2740390..2741619 (+)	1230	WP_152441222.1	type II secretion system F family protein	Machinery gene
FIU82_RS12735 (FIU82_12915)	pilD	2741620..2742516 (+)	897	WP_172972730.1	A24 family peptidase	Machinery gene
FIU82_RS12740 (FIU82_12920)	coaE	2742524..2743129 (+)	606	WP_152441224.1	dephospho-CoA kinase	-
FIU82_RS12745 (FIU82_12925)	pilB	2743218..2744897 (+)	1680	WP_152441225.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FIU82_RS12750 (FIU82_12930)	yacG	2744975..2745214 (+)	240	WP_152441226.1	DNA gyrase inhibitor YacG	-
FIU82_RS12755 (FIU82_12935)	-	2745223..2745996 (-)	774	WP_045979345.1	YggN family protein	-
FIU82_RS12760 (FIU82_12940)	-	2746126..2746944 (-)	819	WP_253946319.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 298 a.a.
• Molecular weight: 32627.98 Da
• Isoelectric Point: 6.4447

>NTDB_id=393912 FIU82_RS12735 WP_172972730.1 2741620..2742516(+) (pilD) [Pseudoalteromonas sp. THAF3]
MFSEVLELMKSEPWFFITTVLFVSLAVGSFLNVVIYRIPKMMQNQWQSECRLLLADELASKSEQSQSASAPFNLLTPAST
CPKCNNKIKPWHNIPLLSWLFLKGQCAYCQAPISIRYPAIELITAVLSLVVALTFGPTQLTVLYIIITWVLVALTFIDID
HMLLPDQLTLPLLWLALLASVAGITIEPSAALIGAAAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAVFGALLGWQALLM
IILLSSIVGAVIGITLLSIQGKDKATPIPFGPYIAIAGWIAMLWGEQIQGAYFGAILG

Nucleotide

• Length: 897 bp

>NTDB_id=393912 FIU82_RS12735 WP_172972730.1 2741620..2742516(+) (pilD) [Pseudoalteromonas sp. THAF3]
ATGTTTTCAGAAGTACTCGAATTAATGAAAAGCGAGCCGTGGTTTTTTATAACCACGGTTCTTTTTGTATCCTTAGCTGT
AGGTAGCTTCCTCAATGTGGTTATTTACCGCATTCCAAAGATGATGCAAAATCAATGGCAGTCAGAGTGTCGCTTACTAC
TTGCCGATGAATTGGCATCAAAAAGTGAACAAAGCCAATCAGCATCCGCTCCTTTTAACCTTCTAACGCCAGCTTCGACT
TGCCCGAAATGTAATAACAAAATCAAGCCTTGGCACAATATCCCCCTGCTCAGTTGGCTATTTTTAAAAGGCCAATGCGC
ATACTGCCAGGCGCCTATCTCTATTCGCTATCCAGCTATAGAGCTCATCACTGCGGTTTTAAGTTTAGTTGTCGCGCTTA
CCTTTGGCCCCACTCAACTAACCGTGCTTTACATCATTATCACCTGGGTATTGGTGGCACTGACATTCATAGACATAGAC
CATATGCTGTTGCCAGATCAGCTGACGTTACCGCTGTTATGGCTGGCTTTATTAGCAAGTGTTGCAGGGATCACCATAGA
ACCGAGTGCAGCACTAATTGGCGCTGCCGCAGGCTACCTTAGCTTATGGAGCGTTTATTGGCTGTTCAAATTGCTGACCG
GTAAAGAAGGGATGGGCTATGGTGACTTTAAACTATTGGCGGTATTTGGTGCCTTATTAGGTTGGCAAGCATTACTGATG
ATCATACTACTGTCCAGCATTGTGGGTGCCGTAATTGGGATTACCTTACTCTCTATTCAAGGCAAAGATAAGGCAACCCC
GATTCCGTTTGGCCCTTATATTGCCATTGCTGGCTGGATAGCCATGCTTTGGGGAGAACAAATCCAAGGCGCTTATTTCG
GCGCTATTTTGGGTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (23-135)

• Type IV leader peptidase family (Peptidase_A24) (146-254)

3D structure

• AlphaFold DB: A0A5P9IZ28

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.381	98.658	0.517
pilD	Vibrio campbellii strain DS40M4	51.361	98.658	0.507
pilD	Acinetobacter nosocomialis M2	53.986	92.617	0.5
pilD	Acinetobacter baumannii D1279779	53.986	92.617	0.5
pilD	Neisseria gonorrhoeae MS11	46.899	86.577	0.406