Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIU82_RS12725 Genome accession   NZ_CP045418
Coordinates   2738619..2740310 (+) Length   563 a.a.
NCBI ID   WP_152441221.1    Uniprot ID   A0A5P9J4D5
Organism   Pseudoalteromonas sp. THAF3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2733619..2745310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU82_RS12690 (FIU82_12870) - 2733749..2734039 (-) 291 WP_022945437.1 cell division protein ZapA -
  FIU82_RS12695 (FIU82_12875) zapB 2734039..2734269 (-) 231 WP_045979239.1 cell division protein ZapB -
  FIU82_RS12700 (FIU82_12880) - 2734517..2735356 (+) 840 WP_151173421.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
  FIU82_RS12705 (FIU82_12885) - 2735369..2736217 (-) 849 WP_253946366.1 cation diffusion facilitator family transporter -
  FIU82_RS12710 (FIU82_12890) trmL 2736398..2736862 (+) 465 WP_152441218.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  FIU82_RS12715 (FIU82_12895) nadC 2736911..2737762 (+) 852 WP_152441219.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FIU82_RS12720 (FIU82_12900) - 2738140..2738556 (+) 417 WP_152441220.1 pilin -
  FIU82_RS12725 (FIU82_12905) pilB 2738619..2740310 (+) 1692 WP_152441221.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU82_RS12730 (FIU82_12910) pilC 2740390..2741619 (+) 1230 WP_152441222.1 type II secretion system F family protein Machinery gene
  FIU82_RS12735 (FIU82_12915) pilD 2741620..2742516 (+) 897 WP_172972730.1 A24 family peptidase Machinery gene
  FIU82_RS12740 (FIU82_12920) coaE 2742524..2743129 (+) 606 WP_152441224.1 dephospho-CoA kinase -
  FIU82_RS12745 (FIU82_12925) pilB 2743218..2744897 (+) 1680 WP_152441225.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU82_RS12750 (FIU82_12930) yacG 2744975..2745214 (+) 240 WP_152441226.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62197.32 Da        Isoelectric Point: 5.5499

>NTDB_id=393910 FIU82_RS12725 WP_152441221.1 2738619..2740310(+) (pilB) [Pseudoalteromonas sp. THAF3]
MNINSPLVRRLSALGLLSPDQISSQAKNQYGSIAEMICNVAKISPEQLQQKASHIFHVSQYDISTFDLSMLPEQGMLNEK
LIKKHKLLPIGAKGKTLFIATADPTDFSAFEDYEFNTGMQTEAVIVRYDHLEKAIDGLFDSTSGLGLTDNDMEELEGVDS
GDGNKKDLATDVNSKEDDAPIIVYINKILMDAIKRGASDLHFEPYEKRYRIRFRVDGVLHEIASPPVNLESRIAARIKVM
SHMDLAEKRKPQDGRIKLKINAKKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALAQPQGMIL
VTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGINQVQISAKSDMTFANALRAFLRQDPDIVMVGEIRDLETA
EISIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPSYNIASSVSLIIAQRLARRLCKHCKEPEQLPQEELLKQGFTHEQL
SDLTVYKPVGCDDCTEGYKGRVGIYEVMPITPKIAEIIMRGGNSLEIAEVAAQEGVNNLRKSGLQKVAQGVTSLVEVNRV
TNM

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=393910 FIU82_RS12725 WP_152441221.1 2738619..2740310(+) (pilB) [Pseudoalteromonas sp. THAF3]
ATGAATATAAATTCTCCTCTTGTTAGAAGGCTATCTGCCCTCGGTTTATTATCACCAGACCAAATATCCTCACAAGCCAA
AAACCAATATGGTTCTATCGCAGAGATGATATGCAACGTTGCAAAAATTTCCCCGGAGCAATTGCAGCAAAAAGCCAGCC
ATATCTTTCATGTTTCCCAATACGACATTTCCACCTTTGATTTGAGCATGCTGCCCGAACAGGGAATGCTAAATGAAAAG
CTGATTAAAAAGCACAAGTTGCTGCCAATAGGTGCGAAAGGAAAAACATTATTCATTGCAACGGCTGACCCTACCGATTT
TAGCGCTTTCGAAGATTACGAATTCAACACGGGAATGCAAACAGAGGCGGTGATTGTTCGTTATGACCATCTAGAAAAAG
CGATCGATGGCTTATTCGATAGTACGTCGGGGCTCGGTCTCACCGACAATGATATGGAAGAGCTCGAAGGTGTTGACTCT
GGTGATGGTAATAAGAAAGATTTAGCAACAGATGTAAACTCTAAAGAAGACGACGCCCCCATTATTGTTTATATCAATAA
AATTTTAATGGATGCGATTAAGCGCGGAGCTTCCGATTTACACTTTGAACCTTATGAAAAACGCTACCGCATTCGTTTTC
GTGTTGATGGCGTGCTCCATGAAATTGCCTCTCCTCCTGTTAACCTAGAGAGCCGTATCGCTGCACGTATCAAAGTTATG
TCGCATATGGACTTGGCGGAGAAGCGCAAACCCCAAGATGGCCGTATAAAACTAAAAATAAACGCCAAAAAAAGTATCGA
CTTCCGTGTGAGTACCCTTCCCACCCTTTGGGGTGAGAAAATCGTAATGCGTATTCTTGATAGTTCCAGTGCGATGCTCG
GCATTGATGTGCTTGGCTATGAGCCTGAGCAAAAACAGCTTTATTTGGACGCACTTGCACAACCCCAGGGGATGATTCTG
GTTACCGGTCCTACAGGTTCAGGTAAAACCGTATCACTTTATACCGGTCTCAATATTCTTAATACCACTGAGCGTAATAT
TAGTACTGCCGAGGACCCCGTTGAAATAAACCTAGAGGGGATTAACCAGGTTCAAATCAGCGCTAAATCTGATATGACCT
TCGCCAATGCTTTACGCGCGTTTTTACGTCAAGACCCTGATATTGTCATGGTGGGTGAGATTCGAGACTTAGAAACAGCG
GAAATATCGATAAAAGCAGCGCAAACAGGCCACTTAGTACTATCGACGCTACACACCAACTCAGCGCCGGAAACTCTGAC
TCGTTTAATGAATATGGGTGTGCCTTCATACAACATTGCCAGTTCAGTGAGTTTGATTATCGCCCAGCGGCTAGCCCGTC
GGTTATGTAAGCATTGCAAAGAGCCTGAACAGCTACCTCAAGAAGAGCTACTAAAGCAAGGGTTTACGCACGAGCAGCTA
AGTGATTTAACTGTCTACAAACCAGTAGGCTGTGATGATTGCACCGAAGGCTATAAAGGCCGAGTTGGTATTTATGAGGT
AATGCCCATCACCCCTAAAATAGCCGAAATTATAATGCGCGGCGGTAACTCGTTAGAAATAGCAGAAGTCGCGGCTCAAG
AAGGTGTCAATAACCTAAGAAAGTCAGGGCTACAAAAGGTTGCTCAGGGCGTGACTAGCCTGGTAGAAGTCAATCGCGTA
ACCAACATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9J4D5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.194

100

0.572
  pilB Acinetobacter baumannii D1279779

59.475

94.671

0.563
  pilB Legionella pneumophila strain ERS1305867

55.243

94.849

0.524
  pilB Vibrio parahaemolyticus RIMD 2210633

51.693

99.645

0.515
  pilB Vibrio campbellii strain DS40M4

50.622

100

0.506
  pilB Vibrio cholerae strain A1552

53.019

94.139

0.499
  pilF Neisseria gonorrhoeae MS11

51.215

95.027

0.487
  pilB/pilB1 Synechocystis sp. PCC 6803

37.34

100

0.414
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.46

100

0.366

Multiple sequence alignment