Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIU82_RS12745 Genome accession   NZ_CP045418
Coordinates   2743218..2744897 (+) Length   559 a.a.
NCBI ID   WP_152441225.1    Uniprot ID   A0A5P9J2M2
Organism   Pseudoalteromonas sp. THAF3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2738218..2749897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU82_RS12725 (FIU82_12905) pilB 2738619..2740310 (+) 1692 WP_152441221.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU82_RS12730 (FIU82_12910) pilC 2740390..2741619 (+) 1230 WP_152441222.1 type II secretion system F family protein Machinery gene
  FIU82_RS12735 (FIU82_12915) pilD 2741620..2742516 (+) 897 WP_172972730.1 A24 family peptidase Machinery gene
  FIU82_RS12740 (FIU82_12920) coaE 2742524..2743129 (+) 606 WP_152441224.1 dephospho-CoA kinase -
  FIU82_RS12745 (FIU82_12925) pilB 2743218..2744897 (+) 1680 WP_152441225.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU82_RS12750 (FIU82_12930) yacG 2744975..2745214 (+) 240 WP_152441226.1 DNA gyrase inhibitor YacG -
  FIU82_RS12755 (FIU82_12935) - 2745223..2745996 (-) 774 WP_045979345.1 YggN family protein -
  FIU82_RS12760 (FIU82_12940) - 2746126..2746944 (-) 819 WP_253946319.1 ABC transporter substrate-binding protein -
  FIU82_RS12765 (FIU82_12945) - 2747014..2748828 (-) 1815 WP_253946320.1 bifunctional diguanylate cyclase/phosphodiesterase -
  FIU82_RS12770 (FIU82_12950) - 2748898..2749416 (-) 519 WP_152441227.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62416.87 Da        Isoelectric Point: 6.0124

>NTDB_id=393913 FIU82_RS12745 WP_152441225.1 2743218..2744897(+) (pilB) [Pseudoalteromonas sp. THAF3]
MNINSPLIRKFITLGRIDSDTVKTHQEQSRCAAETIIRSSNMSNQELLAQCVELFRTPYIDLSSFDPRSIDKHLLNEQLI
RRHHMLPISAKGNKLFLAASDPTDYGAFENYEFSTGLQCEVVLADYRQIDNLIEQLFDATHTLKLSQEDSDELSHLAKQD
YDNHHPEQGNEDDDAPIIVYINKILRDAIRRGASDLHFEPYDGDYRIRFRIDGVLHEMANPPYALSTRLAARIKVMSKLD
ISEKRKPQDGRIKLTISPRNSIDFRVSTLPTMWGEKIVMRVLDSSNTTLGIDALGYEEEQKQLYLDALQQPQGMILVTGP
TGSGKTVSLYTGLSILNTPERNISTAEDPVEINVKGINQVQINPRAELTFSSALRAFLRQDPDVVMVGEIRDLETAEIAI
KAAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSVSLVIAQRLARRLCKHCKEPEQLPRDELLKQGYTESDLTHIS
LFKATGCDQCTDGYKGRVGIYEVMEVTPHIAQTIMDGGNSLDIAKASKEAGCATLRQSGLRKAAEGITSLSEVNRVTRL

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=393913 FIU82_RS12745 WP_152441225.1 2743218..2744897(+) (pilB) [Pseudoalteromonas sp. THAF3]
ATGAATATTAACTCACCTTTGATTCGTAAATTTATTACCTTAGGACGTATCGATAGCGACACGGTAAAGACGCACCAAGA
GCAAAGCCGCTGCGCAGCAGAGACCATAATCCGCAGCAGCAACATGAGTAATCAAGAACTGTTGGCACAATGTGTTGAAC
TCTTTCGCACTCCGTATATTGATTTAAGCTCCTTTGACCCCAGAAGCATAGACAAGCACTTGCTAAATGAGCAGTTGATT
CGTCGTCATCATATGCTGCCCATTTCTGCCAAGGGTAACAAGCTATTTCTCGCCGCCTCAGATCCTACTGACTACGGTGC
TTTTGAAAATTACGAGTTTAGTACCGGGCTTCAATGTGAAGTAGTGCTGGCTGATTATCGGCAAATAGATAACCTCATCG
AGCAGCTGTTCGATGCCACCCACACACTGAAGCTATCGCAAGAGGATAGCGACGAACTCAGCCACCTCGCCAAACAAGAC
TATGATAATCATCATCCGGAACAGGGCAATGAGGATGACGACGCCCCAATCATTGTCTATATCAATAAGATTCTCCGTGA
TGCCATACGCCGAGGTGCTTCTGATTTACACTTTGAGCCCTATGATGGCGACTACCGCATACGCTTTCGAATTGATGGTG
TGTTACATGAGATGGCCAATCCACCTTATGCGCTGTCCACTCGCCTTGCAGCCCGCATCAAAGTAATGTCTAAACTAGAT
ATTTCGGAAAAGCGCAAACCCCAAGATGGCCGCATTAAGCTTACGATTTCGCCGCGTAATAGCATTGATTTTCGGGTCAG
TACGTTGCCAACTATGTGGGGGGAAAAAATTGTTATGCGGGTGCTTGATTCCTCCAACACGACCCTAGGCATTGATGCGC
TTGGCTACGAGGAGGAGCAAAAGCAGCTTTATCTAGATGCATTGCAACAACCTCAAGGCATGATTCTTGTCACCGGCCCC
ACAGGTTCCGGAAAGACCGTCTCTCTATACACTGGACTCAGTATTCTTAACACCCCTGAGCGCAATATTTCTACAGCCGA
AGATCCGGTCGAAATAAACGTTAAGGGCATAAATCAAGTACAGATTAACCCCAGGGCAGAGCTCACTTTTTCTAGCGCTC
TTAGAGCTTTTCTACGCCAAGACCCAGATGTAGTAATGGTGGGCGAGATACGCGATTTAGAAACCGCCGAAATCGCCATT
AAAGCTGCGCAAACCGGGCACTTGGTGCTTTCCACCCTGCATACCAACTCCGCACCTGAGACTATCACGCGACTTTTAAA
TATGGGCGTACCGGCGTACAACGTGGCTAGCTCTGTTTCACTAGTCATCGCCCAACGTTTAGCTCGGCGACTGTGCAAAC
ACTGTAAAGAACCTGAGCAACTCCCTCGTGATGAGTTGCTCAAACAAGGCTACACCGAATCTGACTTAACTCACATAAGT
CTATTCAAAGCGACAGGGTGTGATCAATGTACCGATGGTTACAAAGGTCGGGTGGGAATATACGAGGTGATGGAAGTAAC
TCCCCACATAGCACAAACGATTATGGATGGTGGAAACTCACTTGATATTGCCAAAGCATCAAAAGAAGCAGGCTGTGCGA
CATTGCGTCAATCGGGGCTACGTAAAGCTGCAGAGGGCATTACCTCACTATCTGAGGTTAATCGTGTTACGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(183-450)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9J2M2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.915

100

0.542
  pilB Acinetobacter baylyi ADP1

52.66

100

0.531
  pilB Vibrio cholerae strain A1552

55.263

95.17

0.526
  pilB Legionella pneumophila strain ERS1305867

55.787

94.275

0.526
  pilB Vibrio parahaemolyticus RIMD 2210633

52.652

94.454

0.497
  pilB Vibrio campbellii strain DS40M4

52.068

95.17

0.496
  pilF Neisseria gonorrhoeae MS11

48.643

92.308

0.449
  pilF Thermus thermophilus HB27

37.857

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.143

100

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

39.306

92.844

0.365

Multiple sequence alignment