Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   GB851_RS00275 Genome accession   NZ_CP045141
Coordinates   55732..56205 (+) Length   157 a.a.
NCBI ID   WP_164538010.1    Uniprot ID   -
Organism   Kingella kingae strain F41215CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 50732..61205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GB851_RS00255 (GB851_00265) - 51084..52133 (-) 1050 WP_038310306.1 metallophosphoesterase -
  GB851_RS00260 (GB851_00270) - 52124..52669 (-) 546 WP_038310304.1 gamma carbonic anhydrase family protein -
  GB851_RS00265 (GB851_00275) - 52709..53443 (-) 735 WP_003791735.1 histidine phosphatase family protein -
  GB851_RS00270 (GB851_00280) recJ 53660..55372 (-) 1713 WP_038310302.1 single-stranded-DNA-specific exonuclease RecJ -
  GB851_RS00275 (GB851_00285) pilE 55732..56205 (+) 474 WP_164538010.1 pilin Machinery gene
  GB851_RS00280 (GB851_00290) - 56352..56780 (+) 429 WP_019390096.1 pilin -
  GB851_RS00285 (GB851_00295) - 56792..57565 (+) 774 WP_164538011.1 fimb protein -
  GB851_RS00295 (GB851_00305) - 58015..58905 (-) 891 WP_003788327.1 metal ABC transporter permease -
  GB851_RS00300 (GB851_00310) - 58941..59333 (-) 393 WP_038302564.1 pyrimidine dimer DNA glycosylase/endonuclease V -
  GB851_RS00305 (GB851_00315) - 59382..60107 (-) 726 WP_038302563.1 metal ABC transporter ATP-binding protein -
  GB851_RS00310 - 60110..60268 (-) 159 WP_164538012.1 hypothetical protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16343.50 Da        Isoelectric Point: 9.0142

>NTDB_id=392269 GB851_RS00275 WP_164538010.1 55732..56205(+) (pilE) [Kingella kingae strain F41215CHC]
MKTMQKGFTLIELMIVIAIIGILAAIALPAYQDYTKRSRVSEGLSLAGGAKSALTEYYASNNEWPENNDKAGIAPANKIT
GNAVKSVEVARDTAGATGKGKITVTFNGKVKEGANLILEGTGNEAGSVQWTCTGGNLDAKFRPSECRNGTTAATNGS

Nucleotide


Download         Length: 474 bp        

>NTDB_id=392269 GB851_RS00275 WP_164538010.1 55732..56205(+) (pilE) [Kingella kingae strain F41215CHC]
ATGAAAACTATGCAAAAAGGTTTCACATTAATCGAGTTGATGATCGTAATCGCTATTATCGGTATTTTGGCTGCGATTGC
ATTGCCAGCTTACCAAGATTACACTAAACGTTCACGTGTATCTGAAGGTTTGAGCTTGGCTGGTGGTGCGAAAAGTGCTT
TGACTGAATACTACGCTTCTAACAATGAATGGCCTGAAAACAACGATAAAGCTGGTATTGCACCTGCTAATAAAATCACA
GGTAATGCAGTAAAAAGCGTAGAAGTTGCTCGTGATACTGCTGGTGCAACTGGTAAAGGTAAAATTACTGTTACATTTAA
CGGAAAAGTTAAAGAAGGCGCAAATTTGATTTTGGAAGGTACTGGTAACGAAGCTGGTTCGGTACAATGGACTTGTACTG
GTGGTAACTTGGATGCTAAATTCCGTCCTTCTGAATGCCGTAATGGTACTACTGCCGCTACCAATGGTAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(37-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

48.824

100

0.529
  pilE Neisseria gonorrhoeae strain FA1090

47.024

100

0.503
  pilA2 Legionella pneumophila strain ERS1305867

50.694

91.72

0.465
  pilA2 Legionella pneumophila str. Paris

50

91.72

0.459
  pilA Ralstonia pseudosolanacearum GMI1000

40.351

100

0.439
  comP Acinetobacter baylyi ADP1

46.259

93.631

0.433
  pilA Haemophilus influenzae 86-028NP

43.836

92.994

0.408
  pilA Acinetobacter baumannii strain A118

42.568

94.268

0.401
  pilA/pilA1 Eikenella corrodens VA1

39.375

100

0.401
  pilA Haemophilus influenzae Rd KW20

42.857

93.631

0.401
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.667

95.541

0.389

Multiple sequence alignment