Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FBAL_RS01915 Genome accession   NC_014541
Coordinates   397839..399401 (-) Length   520 a.a.
NCBI ID   WP_013343883.1    Uniprot ID   -
Organism   Ferrimonas balearica DSM 9799     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 392839..404401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBAL_RS01890 (Fbal_0358) yacG 394071..394295 (-) 225 WP_013343878.1 DNA gyrase inhibitor YacG -
  FBAL_RS01895 (Fbal_0359) - 394292..395041 (-) 750 WP_013343879.1 cell division protein ZapD -
  FBAL_RS01900 (Fbal_0360) coaE 395025..395648 (-) 624 WP_013343880.1 dephospho-CoA kinase -
  FBAL_RS01905 (Fbal_0361) pilD 395645..396526 (-) 882 WP_013343881.1 A24 family peptidase Machinery gene
  FBAL_RS01910 (Fbal_0362) pilC 396563..397822 (-) 1260 WP_013343882.1 type II secretion system F family protein Machinery gene
  FBAL_RS01915 (Fbal_0363) pilB 397839..399401 (-) 1563 WP_013343883.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FBAL_RS01920 (Fbal_0364) - 399475..399903 (-) 429 WP_013343884.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FBAL_RS01925 (Fbal_0365) nadC 400171..401028 (-) 858 WP_013343885.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FBAL_RS01930 (Fbal_0366) - 401035..401550 (-) 516 WP_013343886.1 TIGR02281 family clan AA aspartic protease -
  FBAL_RS01935 (Fbal_0367) ampD 401620..402222 (+) 603 WP_013343887.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FBAL_RS01940 (Fbal_0368) ampE 402222..403073 (+) 852 WP_013343888.1 beta-lactamase regulator AmpE -
  FBAL_RS01945 (Fbal_0369) pdhR 403279..404034 (+) 756 WP_013343889.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 520 a.a.        Molecular weight: 57785.34 Da        Isoelectric Point: 5.3864

>NTDB_id=38423 FBAL_RS01915 WP_013343883.1 397839..399401(-) (pilB) [Ferrimonas balearica DSM 9799]
MLEPANVNIQMKPNFGIPQMDVSYLVTDFIEDSFLNTKLIEKHNAVPLTVRDNRLLIAIADPDNFEALEDFKFNLGYHAE
PIQVDKNSLDKLISELLQEDLGQLEITEEDEKNLAHIDTEDDKKQDDVSGADDAPIVVFLNKIMTEAIRKGASDLHFEPY
ENEYRIRFRIDGMLHTVNTPPVGLGPRLSARIKVMSRLDLAERRLPQDGRIKLKVGKKTMIDFRVSTLPTLYGEKVVMRI
LDSSAAKLNIDILGFSDAQKDLYLKNLHRPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLPGINQVQ
IHPKAGLSFASALRAFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVMSTLHTNSAAETLTRMLNMGVPAYNIASSVNL
IMAQRLCRKLCPKCRQPESIPHEELLREGFSPGQLHTQPTIYRAVGCDDCTDGYKGRVGLYELMPMSKAISDLIMNNGSS
LELEAQANREGMVSLRHSGLLKVLEGVTSLAEVNRVTIAH

Nucleotide


Download         Length: 1563 bp        

>NTDB_id=38423 FBAL_RS01915 WP_013343883.1 397839..399401(-) (pilB) [Ferrimonas balearica DSM 9799]
ATGCTCGAACCAGCTAACGTCAATATCCAAATGAAACCAAACTTTGGCATTCCACAGATGGATGTCAGCTACTTGGTGAC
TGACTTTATTGAAGACAGTTTTCTCAATACTAAACTCATTGAGAAACACAATGCCGTACCCTTGACAGTAAGGGACAACC
GATTACTGATAGCCATCGCAGATCCGGATAACTTTGAAGCTTTGGAAGACTTCAAGTTTAATCTTGGATATCACGCTGAG
CCCATTCAGGTTGATAAAAATTCTCTCGACAAATTAATCTCCGAGTTGCTTCAAGAAGATTTAGGACAGCTCGAGATTAC
AGAGGAAGATGAGAAAAATCTTGCTCACATTGATACCGAAGACGACAAGAAGCAAGACGATGTAAGTGGGGCAGACGATG
CCCCCATTGTGGTTTTTCTCAATAAAATTATGACGGAAGCGATTCGTAAGGGCGCCTCGGATCTCCATTTTGAACCCTAC
GAAAACGAGTACCGCATCCGTTTCCGTATTGATGGTATGCTGCACACCGTCAATACCCCACCGGTGGGGCTTGGGCCCCG
TCTGTCTGCCCGCATTAAGGTGATGTCCAGGCTGGACCTGGCCGAACGCCGCCTGCCCCAGGATGGTCGCATCAAGCTGA
AGGTGGGCAAAAAAACCATGATCGATTTTCGTGTCTCCACCCTGCCCACCCTCTACGGGGAGAAGGTGGTGATGCGTATT
CTCGACTCCTCCGCCGCCAAGCTGAATATCGATATTCTGGGTTTCAGCGACGCGCAGAAAGATCTCTATCTGAAAAATCT
GCACCGGCCCCAGGGGATGATCCTGGTCACCGGCCCCACTGGTTCCGGCAAAACCGTCTCACTCTATACCGGTCTCAATA
TCCTCAATACCGAGGAGCGCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCTGGGATCAACCAGGTGCAG
ATCCACCCCAAGGCAGGGCTCTCCTTTGCTTCGGCCCTTCGCGCCTTTCTGCGTCAGGACCCGGATATCGTGATGGTGGG
GGAGATCCGGGATCTGGAAACTGCTGAGATCGCCGTTAAAGCGGCGCAAACCGGCCACCTGGTGATGTCCACCCTGCACA
CCAACTCCGCCGCCGAAACCCTGACCCGGATGCTGAATATGGGCGTGCCCGCCTACAACATCGCCAGTTCGGTCAACCTG
ATTATGGCTCAGCGTCTGTGCCGTAAGCTCTGCCCCAAGTGCCGGCAGCCGGAAAGCATCCCTCACGAAGAGCTGCTTCG
GGAAGGCTTCAGTCCCGGGCAGCTGCATACCCAACCGACCATCTACCGGGCCGTGGGCTGCGACGACTGCACCGATGGCT
ACAAGGGCCGGGTTGGCCTCTATGAACTGATGCCGATGAGCAAGGCCATTTCTGACCTTATTATGAACAATGGCTCCTCG
CTGGAGCTGGAAGCCCAGGCCAACCGGGAAGGGATGGTCAGCCTGCGCCACTCCGGGCTGCTTAAGGTGCTGGAAGGGGT
CACCTCGCTGGCAGAAGTTAACCGGGTCACCATCGCCCACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-99)

Type II/IV secretion system protein (T2SSE)

(142-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.619

100

0.562
  pilB Acinetobacter baylyi ADP1

55.644

97.115

0.54
  pilB Acinetobacter baumannii D1279779

53.654

100

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

53.831

97.885

0.527
  pilB Vibrio cholerae strain A1552

52.611

99.423

0.523
  pilB Vibrio campbellii strain DS40M4

55.67

93.269

0.519
  pilF Neisseria gonorrhoeae MS11

53.926

93.077

0.502
  pilF Thermus thermophilus HB27

38.826

100

0.394
  pilB/pilB1 Synechocystis sp. PCC 6803

39.802

97.115

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.632

91.923

0.383
  pilB Haemophilus influenzae Rd KW20

39.315

95.385

0.375
  pilB Haemophilus influenzae 86-028NP

39.468

94.038

0.371

Multiple sequence alignment