Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   FXF61_RS09835 Genome accession   NZ_CP043320
Coordinates   2160937..2161458 (+) Length   173 a.a.
NCBI ID   WP_151185092.1    Uniprot ID   A0A5J6Q9K3
Organism   Pseudomonas sp. C27(2019)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2155937..2166458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXF61_RS09820 (FXF61_09820) pilD 2156396..2157265 (-) 870 WP_151185089.1 A24 family peptidase Machinery gene
  FXF61_RS09825 (FXF61_09825) pilC 2157658..2158875 (-) 1218 WP_151185090.1 type II secretion system F family protein Machinery gene
  FXF61_RS09830 (FXF61_09830) pilB 2158879..2160573 (-) 1695 WP_151185091.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FXF61_RS09835 (FXF61_09835) pilE 2160937..2161458 (+) 522 WP_151185092.1 pilin Machinery gene
  FXF61_RS09840 (FXF61_09840) - 2161570..2162103 (+) 534 WP_151185093.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FXF61_RS09845 (FXF61_09845) tfpZ 2162145..2162879 (+) 735 WP_151185094.1 TfpX/TfpZ family type IV pilin accessory protein -
  FXF61_RS15095 (FXF61_09850) - 2163001..2163321 (+) 321 WP_151185095.1 excalibur calcium-binding domain-containing protein -
  FXF61_RS09855 (FXF61_09855) - 2163436..2163990 (-) 555 WP_151185096.1 HEPN domain-containing protein -
  FXF61_RS09860 (FXF61_09860) - 2164182..2165177 (+) 996 WP_151185097.1 IS481 family transposase -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17754.20 Da        Isoelectric Point: 4.3224

>NTDB_id=382868 FXF61_RS09835 WP_151185092.1 2160937..2161458(+) (pilE) [Pseudomonas sp. C27(2019)]
MKAQMQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSKISEALIQASSPKGLISEAFQTDGLDGVEAAATTYNAIPAN
EKETKYVADITIDDTNGAITVTLQGATAGFPTDVQGATIVLTPNVKKAGLTAGATGAIDWACASDSATTAGNRGLTAAEL
GTLEAKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=382868 FXF61_RS09835 WP_151185092.1 2160937..2161458(+) (pilE) [Pseudomonas sp. C27(2019)]
ATGAAAGCACAAATGCAAAAGGGTTTTACCCTAATTGAGTTAATGATCGTTGTGGCGATCATCGGTATTTTGGCTGCGAT
CGCGCTGCCTGCGTATCAAGACTACACTGTACGCTCAAAAATCTCAGAAGCTCTAATTCAAGCTTCATCACCTAAAGGTT
TGATTAGTGAAGCATTTCAAACTGACGGTCTTGACGGTGTTGAAGCAGCAGCAACAACATATAACGCAATACCTGCCAAT
GAAAAAGAAACTAAATACGTAGCGGACATTACAATTGATGATACTAACGGTGCTATTACAGTAACTTTACAAGGTGCAAC
AGCTGGTTTCCCTACTGACGTACAAGGAGCAACAATTGTACTGACTCCTAATGTTAAGAAAGCTGGTTTAACAGCAGGTG
CAACCGGTGCAATTGACTGGGCTTGCGCATCTGACTCTGCAACAACAGCTGGCAACCGTGGTTTAACAGCAGCCGAGTTG
GGTACTCTTGAAGCAAAATACGCTCCAGCAGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6Q9K3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

55.556

100

0.607
  pilA Ralstonia pseudosolanacearum GMI1000

41.667

100

0.434
  pilA2 Legionella pneumophila str. Paris

42.857

100

0.434
  pilA2 Legionella pneumophila strain ERS1305867

42.286

100

0.428
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.04

100

0.41
  comP Acinetobacter baylyi ADP1

39.08

100

0.393
  pilA/pilA1 Eikenella corrodens VA1

40.244

94.798

0.382

Multiple sequence alignment