Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FXF61_RS09825 Genome accession   NZ_CP043320
Coordinates   2157658..2158875 (-) Length   405 a.a.
NCBI ID   WP_151185090.1    Uniprot ID   A0A5J6QF06
Organism   Pseudomonas sp. C27(2019)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2152658..2163875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXF61_RS09805 (FXF61_09805) - 2154425..2154673 (-) 249 WP_151185086.1 hypothetical protein -
  FXF61_RS09810 (FXF61_09810) nth 2155062..2155700 (+) 639 WP_151185087.1 endonuclease III -
  FXF61_RS09815 (FXF61_09815) - 2155810..2155995 (+) 186 WP_178087293.1 PA3496 family putative envelope integrity protein -
  FXF61_RS09820 (FXF61_09820) pilD 2156396..2157265 (-) 870 WP_151185089.1 A24 family peptidase Machinery gene
  FXF61_RS09825 (FXF61_09825) pilC 2157658..2158875 (-) 1218 WP_151185090.1 type II secretion system F family protein Machinery gene
  FXF61_RS09830 (FXF61_09830) pilB 2158879..2160573 (-) 1695 WP_151185091.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FXF61_RS09835 (FXF61_09835) pilE 2160937..2161458 (+) 522 WP_151185092.1 pilin Machinery gene
  FXF61_RS09840 (FXF61_09840) - 2161570..2162103 (+) 534 WP_151185093.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FXF61_RS09845 (FXF61_09845) tfpZ 2162145..2162879 (+) 735 WP_151185094.1 TfpX/TfpZ family type IV pilin accessory protein -
  FXF61_RS15095 (FXF61_09850) - 2163001..2163321 (+) 321 WP_151185095.1 excalibur calcium-binding domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43813.18 Da        Isoelectric Point: 9.5237

>NTDB_id=382866 FXF61_RS09825 WP_151185090.1 2157658..2158875(-) (pilC) [Pseudomonas sp. C27(2019)]
MAAKTPKTNMYKWEGKNRQGGTMSGQMAGQDAALVKAQLRKQGINPTKVRKKGIELFSAGKKITPMDVAIFTRQMATMMK
AGVPLLQSFDIIAEGLENPNMRKLVDTVKQDVAAGNSFAVSLRKHPQYFDDLYCNLVDSGEQSGALETLLDRVATYKEKT
EALKAKIKKAMNYPMAVVAVAVIVTAILLIKVVPQFQDVFSSFGAELPAFTQFVIGISEFVQSDGWLIVVGFIAFVFAFR
HAHRTSEKFRDSIDRALLKSPVIGNIIYNSSVARFGRTLATTFAAGVPLVEALDSVAGATGNVVFKNAVMKVKSDVSSGM
QLNFSMRSTGVFPSMALQMTAIGEESGSLDEMLDKVATHYEAEVDNAVDGLTALMEPIIMSVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=382866 FXF61_RS09825 WP_151185090.1 2157658..2158875(-) (pilC) [Pseudomonas sp. C27(2019)]
ATGGCGGCGAAAACACCAAAAACCAATATGTACAAGTGGGAAGGCAAAAACCGCCAGGGTGGGACGATGTCTGGGCAAAT
GGCCGGACAGGATGCTGCGCTGGTCAAGGCTCAGTTGCGTAAACAGGGCATTAACCCAACTAAAGTGCGTAAAAAGGGCA
TTGAGCTATTCAGTGCGGGTAAAAAGATCACACCGATGGACGTTGCGATTTTTACTCGACAAATGGCCACGATGATGAAA
GCTGGTGTGCCGCTGTTGCAGTCGTTTGACATCATTGCTGAGGGCTTAGAAAACCCAAATATGCGCAAGCTGGTGGATAC
GGTGAAGCAGGATGTAGCTGCGGGTAATAGTTTTGCTGTGTCCTTGCGTAAGCATCCGCAATACTTTGATGATCTGTATT
GCAATCTGGTGGATTCTGGTGAGCAGTCCGGTGCGCTGGAAACCTTGCTGGATCGGGTCGCCACTTATAAAGAAAAAACT
GAAGCGCTAAAAGCAAAAATCAAAAAAGCCATGAATTATCCAATGGCGGTTGTCGCGGTGGCGGTGATTGTAACGGCTAT
TTTGCTGATTAAAGTGGTGCCGCAGTTTCAGGATGTGTTTTCTAGTTTTGGTGCGGAGTTGCCTGCTTTTACTCAGTTTG
TGATTGGTATTTCTGAGTTTGTGCAAAGTGATGGTTGGTTGATTGTGGTGGGCTTTATTGCGTTTGTTTTTGCGTTTAGA
CATGCGCACCGCACCTCAGAAAAGTTTCGTGACAGCATTGATCGGGCTTTACTGAAATCACCGGTGATCGGCAATATTAT
TTATAACTCTTCTGTGGCGCGTTTTGGCCGTACTTTGGCGACAACTTTCGCGGCGGGTGTGCCTTTGGTGGAGGCGCTGG
ATTCAGTCGCCGGTGCCACCGGTAACGTGGTGTTTAAGAATGCTGTGATGAAAGTGAAGTCGGATGTGTCCAGCGGTATG
CAGCTGAATTTCTCCATGCGCAGCACCGGTGTGTTTCCATCAATGGCTTTGCAGATGACGGCCATTGGTGAGGAGTCTGG
TTCGCTGGATGAAATGCTCGATAAGGTGGCGACACACTATGAGGCTGAAGTGGATAACGCTGTTGATGGCTTAACTGCGT
TAATGGAGCCGATTATTATGTCGGTGCTTGGCGTGTTGGTGGGCGGTTTGATTATTGCCATGTACCTGCCGATCTTCCAG
TTGGGTGCGGTTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6QF06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.531

100

0.775
  pilC Acinetobacter baylyi ADP1

57.985

100

0.583
  pilC Acinetobacter baumannii D1279779

58.438

98.025

0.573
  pilC Legionella pneumophila strain ERS1305867

52.826

100

0.531
  pilG Neisseria meningitidis 44/76-A

43.5

98.765

0.43
  pilG Neisseria gonorrhoeae MS11

43.5

98.765

0.43
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

39.655

100

0.398
  pilC Thermus thermophilus HB27

39.25

98.765

0.388

Multiple sequence alignment