Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FXF61_RS09820 Genome accession   NZ_CP043320
Coordinates   2156396..2157265 (-) Length   289 a.a.
NCBI ID   WP_151185089.1    Uniprot ID   A0A5J6QBP1
Organism   Pseudomonas sp. C27(2019)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2151396..2162265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXF61_RS09805 (FXF61_09805) - 2154425..2154673 (-) 249 WP_151185086.1 hypothetical protein -
  FXF61_RS09810 (FXF61_09810) nth 2155062..2155700 (+) 639 WP_151185087.1 endonuclease III -
  FXF61_RS09815 (FXF61_09815) - 2155810..2155995 (+) 186 WP_178087293.1 PA3496 family putative envelope integrity protein -
  FXF61_RS09820 (FXF61_09820) pilD 2156396..2157265 (-) 870 WP_151185089.1 A24 family peptidase Machinery gene
  FXF61_RS09825 (FXF61_09825) pilC 2157658..2158875 (-) 1218 WP_151185090.1 type II secretion system F family protein Machinery gene
  FXF61_RS09830 (FXF61_09830) pilB 2158879..2160573 (-) 1695 WP_151185091.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FXF61_RS09835 (FXF61_09835) pilE 2160937..2161458 (+) 522 WP_151185092.1 pilin Machinery gene
  FXF61_RS09840 (FXF61_09840) - 2161570..2162103 (+) 534 WP_151185093.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 32304.35 Da        Isoelectric Point: 6.3695

>NTDB_id=382865 FXF61_RS09820 WP_151185089.1 2156396..2157265(-) (pilD) [Pseudomonas sp. C27(2019)]
MAIIEFWGSNIWVFAISAAILGLLVGSFLNVLIYRLPKMLMRDWRKQAHEVLEMPAQDNAETFNLVLPHSSCPHCGHEIK
PWENIPLFSWLFLRGKCSGCSERISIRYPLVELACGVLSAVVAWHYGVSWETLTMLVLTWGLLAMSLIDADHQILPDILV
LPLLWLGLILNHFDLFTSLPDALWGAIIGYMSLWSIFWLFKLVTGKEGMGYGDFKLLAMIGAWGGWQVLPLTILLSSVVG
AVLGIIILRVQRNSMSNPIPFGPYLAVAGWIALIWGDTITSSYLQFAGF

Nucleotide


Download         Length: 870 bp        

>NTDB_id=382865 FXF61_RS09820 WP_151185089.1 2156396..2157265(-) (pilD) [Pseudomonas sp. C27(2019)]
ATGGCGATTATTGAGTTTTGGGGTAGTAATATTTGGGTGTTTGCCATCAGCGCAGCAATCTTAGGGTTGTTGGTGGGTAG
TTTTTTGAATGTGCTGATTTATCGCTTACCCAAGATGCTGATGCGCGATTGGCGCAAGCAAGCGCATGAAGTGCTGGAAA
TGCCTGCGCAAGACAATGCAGAGACGTTTAACCTGGTTCTGCCCCACTCAAGTTGCCCACATTGCGGACATGAAATCAAA
CCGTGGGAAAATATTCCGTTGTTTAGCTGGTTATTCTTACGCGGCAAATGTTCCGGCTGCAGTGAGCGCATTAGTATTCG
TTACCCGCTAGTCGAGCTGGCCTGTGGTGTTTTATCTGCGGTGGTGGCTTGGCATTATGGTGTCAGTTGGGAAACCTTAA
CGATGCTGGTGCTGACCTGGGGTTTGCTAGCGATGAGCCTGATTGATGCAGACCATCAAATCCTCCCTGATATTTTAGTG
CTGCCGCTGCTGTGGCTAGGGTTGATTCTCAATCATTTTGACCTGTTCACTAGCCTGCCTGATGCGCTGTGGGGCGCGAT
CATCGGTTATATGAGCCTCTGGTCGATCTTCTGGCTGTTTAAGCTGGTCACCGGCAAAGAGGGCATGGGCTATGGTGACT
TTAAGTTGCTGGCGATGATTGGCGCTTGGGGTGGCTGGCAGGTGTTGCCGCTGACGATCTTATTGTCTTCGGTAGTGGGT
GCGGTGTTAGGTATTATTATTCTGCGCGTGCAGCGCAATAGCATGAGTAATCCAATCCCTTTTGGCCCGTACTTGGCCGT
GGCTGGCTGGATCGCTTTGATCTGGGGTGACACAATCACCAGCAGCTATTTGCAGTTTGCGGGCTTCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(136-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6QBP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52

95.156

0.495
  pilD Vibrio campbellii strain DS40M4

51.273

95.156

0.488
  pilD Acinetobacter baumannii D1279779

49.296

98.27

0.484
  pilD Acinetobacter nosocomialis M2

49.296

98.27

0.484
  pilD Neisseria gonorrhoeae MS11

51.711

91.003

0.471

Multiple sequence alignment