Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FXF61_RS09830 Genome accession   NZ_CP043320
Coordinates   2158879..2160573 (-) Length   564 a.a.
NCBI ID   WP_151185091.1    Uniprot ID   A0A5J6QAA7
Organism   Pseudomonas sp. C27(2019)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2153879..2165573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXF61_RS09805 (FXF61_09805) - 2154425..2154673 (-) 249 WP_151185086.1 hypothetical protein -
  FXF61_RS09810 (FXF61_09810) nth 2155062..2155700 (+) 639 WP_151185087.1 endonuclease III -
  FXF61_RS09815 (FXF61_09815) - 2155810..2155995 (+) 186 WP_178087293.1 PA3496 family putative envelope integrity protein -
  FXF61_RS09820 (FXF61_09820) pilD 2156396..2157265 (-) 870 WP_151185089.1 A24 family peptidase Machinery gene
  FXF61_RS09825 (FXF61_09825) pilC 2157658..2158875 (-) 1218 WP_151185090.1 type II secretion system F family protein Machinery gene
  FXF61_RS09830 (FXF61_09830) pilB 2158879..2160573 (-) 1695 WP_151185091.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FXF61_RS09835 (FXF61_09835) pilE 2160937..2161458 (+) 522 WP_151185092.1 pilin Machinery gene
  FXF61_RS09840 (FXF61_09840) - 2161570..2162103 (+) 534 WP_151185093.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FXF61_RS09845 (FXF61_09845) tfpZ 2162145..2162879 (+) 735 WP_151185094.1 TfpX/TfpZ family type IV pilin accessory protein -
  FXF61_RS15095 (FXF61_09850) - 2163001..2163321 (+) 321 WP_151185095.1 excalibur calcium-binding domain-containing protein -
  FXF61_RS09855 (FXF61_09855) - 2163436..2163990 (-) 555 WP_151185096.1 HEPN domain-containing protein -
  FXF61_RS09860 (FXF61_09860) - 2164182..2165177 (+) 996 WP_151185097.1 IS481 family transposase -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62095.08 Da        Isoelectric Point: 5.0575

>NTDB_id=382867 FXF61_RS09830 WP_151185091.1 2158879..2160573(-) (pilB) [Pseudomonas sp. C27(2019)]
MSSSMNLIGLARQVVEAGLLDENKVQEAVQQAKRNQTLLVTWLVQNKLVKSRALMELAADQFGVAYFDLNAVNPDVFPQD
LVSERLARQHRVLPLYKRGSKLFIAISDPGNHQAINDVQFSSGMTVEPLLVEDDKLGLAIDRLYQSATGGLEGMDDAELE
GLDFEETESSGPAQTEEADETPVVRFVNKMLLDAIKGGSSDLHFEPYEKMYRVRFRTDGVLHEVAKPPIQLRDRISARLK
VMANMDISERRKPQDGRIKLKVSANKSIDFRVNTLPTLWGEKIVMRILDASSAQMGIDALGYEEPQKELYMEALSQPQGM
ILVTGPTGSGKTVSLYTGLNILNTIDVNISTAEDPVEINLEGINQVNVNPKQGMDFSTALRAFLRQDPDVIMVGEIRDLE
TAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNLATSVNLIIAQRLARKLCACKQELNVPDDVLLKEGFSAEQ
VGTFKVYGPKGCDNCNGGYKGRVGIYEVVKSTPALQRIIMEDGNAIDIDAQMRKDGFNNLRASGLLKVIQGITSLAEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=382867 FXF61_RS09830 WP_151185091.1 2158879..2160573(-) (pilB) [Pseudomonas sp. C27(2019)]
ATGAGTAGTAGTATGAATTTGATCGGCTTGGCTCGACAGGTGGTCGAGGCAGGCTTGCTGGATGAGAATAAAGTCCAAGA
GGCAGTGCAGCAGGCCAAGCGCAACCAAACCTTATTGGTTACCTGGTTGGTGCAAAATAAATTAGTCAAAAGTCGCGCAT
TGATGGAGTTGGCCGCAGATCAGTTTGGCGTGGCTTATTTTGATTTAAATGCGGTGAATCCTGATGTTTTTCCGCAAGAT
TTGGTCAGTGAAAGGCTCGCTCGTCAACATCGCGTACTGCCGTTGTATAAGCGCGGCAGTAAGCTTTTTATCGCTATTTC
TGATCCGGGTAATCATCAAGCCATTAATGATGTGCAGTTCAGCAGCGGTATGACGGTTGAGCCGTTGTTGGTTGAAGACG
ATAAGTTGGGGCTAGCCATTGACAGGCTGTATCAGTCGGCTACCGGCGGTCTAGAAGGGATGGATGACGCTGAGCTTGAA
GGTTTGGATTTTGAAGAAACTGAATCCAGCGGGCCGGCACAAACCGAGGAGGCTGATGAAACGCCGGTGGTGCGTTTTGT
TAATAAAATGCTGCTTGATGCCATTAAAGGGGGCTCTTCAGATTTACACTTTGAGCCTTATGAGAAGATGTACCGCGTGC
GTTTTCGTACCGATGGGGTCTTGCATGAGGTGGCCAAGCCGCCGATTCAATTGCGTGATCGTATCTCGGCCCGCTTAAAA
GTGATGGCCAATATGGATATTTCTGAACGCCGTAAGCCGCAAGATGGGCGGATTAAACTCAAAGTCTCGGCCAATAAATC
CATCGACTTTCGTGTTAACACCCTGCCAACCCTCTGGGGCGAGAAAATCGTGATGCGGATTCTCGATGCCAGCAGCGCGC
AAATGGGCATTGATGCGTTAGGTTATGAGGAGCCGCAGAAAGAGCTGTATATGGAAGCTTTGAGTCAGCCGCAAGGCATG
ATTTTAGTGACGGGGCCGACCGGTTCCGGTAAAACAGTATCGTTGTATACCGGCTTGAATATTCTCAATACCATCGATGT
CAATATTTCTACCGCCGAAGATCCGGTTGAAATTAACTTGGAGGGGATTAACCAGGTTAACGTCAATCCCAAGCAGGGTA
TGGATTTCTCCACTGCGCTGCGCGCCTTCTTGCGGCAAGATCCGGATGTGATCATGGTCGGTGAGATTCGTGATTTAGAA
ACTGCAGAGATCGCGATTAAAGCGGCGCAAACCGGGCACATGGTGATGTCGACGTTGCACACCAACAGTGCTGCGGAAAC
CTTAACGCGTTTGCGCAACATGGGGGTGGCAGCGTTTAACTTGGCCACCTCGGTGAACTTGATTATTGCCCAGCGGCTGG
CACGTAAACTCTGTGCCTGTAAGCAAGAGCTTAATGTGCCTGACGATGTGTTGCTAAAAGAAGGGTTTAGCGCAGAGCAG
GTTGGTACCTTTAAAGTGTATGGACCAAAAGGCTGTGATAATTGCAATGGTGGTTATAAAGGCCGTGTTGGAATATATGA
AGTGGTTAAAAGCACGCCGGCCCTACAAAGGATTATCATGGAAGATGGCAACGCCATAGATATTGATGCGCAGATGCGTA
AAGATGGTTTTAATAATTTGCGCGCATCGGGGTTGCTGAAAGTCATTCAGGGTATTACCAGCCTTGCTGAAGTTAACCGT
GTAACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6QAA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.319

100

0.59
  pilB Acinetobacter baylyi ADP1

58.007

99.645

0.578
  pilB Legionella pneumophila strain ERS1305867

52.113

100

0.525
  pilB Vibrio campbellii strain DS40M4

49.911

99.468

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.644

99.645

0.495
  pilB Vibrio cholerae strain A1552

49.554

99.468

0.493
  pilF Neisseria gonorrhoeae MS11

48.577

99.645

0.484
  pilB/pilB1 Synechocystis sp. PCC 6803

36.02

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.95

98.582

0.374
  pilF Thermus thermophilus HB27

38.225

97.872

0.374

Multiple sequence alignment