Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FXN65_RS04440 Genome accession   NZ_CP043311
Coordinates   919577..920794 (-) Length   405 a.a.
NCBI ID   WP_151131893.1    Uniprot ID   A0A5J6QG59
Organism   Metapseudomonas lalkuanensis strain PE08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 914577..925794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXN65_RS04395 (FXN65_04395) - 914951..915124 (-) 174 WP_151131884.1 DUF3094 family protein -
  FXN65_RS04400 (FXN65_04400) - 915213..915839 (-) 627 WP_151131885.1 DUF1780 domain-containing protein -
  FXN65_RS04405 (FXN65_04405) - 915973..916395 (+) 423 WP_151131886.1 GNAT family N-acetyltransferase -
  FXN65_RS04410 (FXN65_04410) - 916400..916867 (+) 468 WP_151131887.1 FAD/FMN-containing dehydrogenase -
  FXN65_RS04415 (FXN65_04415) - 916864..917553 (+) 690 WP_151131888.1 energy-coupling factor ABC transporter permease -
  FXN65_RS04420 (FXN65_04420) - 917672..917887 (+) 216 WP_151131889.1 hypothetical protein -
  FXN65_RS04425 (FXN65_04425) yacG 917897..918097 (-) 201 WP_151131890.1 DNA gyrase inhibitor YacG -
  FXN65_RS04430 (FXN65_04430) coaE 918094..918705 (-) 612 WP_151131891.1 dephospho-CoA kinase -
  FXN65_RS04435 (FXN65_04435) pilD 918702..919574 (-) 873 WP_151131892.1 A24 family peptidase Machinery gene
  FXN65_RS04440 (FXN65_04440) pilC 919577..920794 (-) 1218 WP_151131893.1 type II secretion system F family protein Machinery gene
  FXN65_RS04445 (FXN65_04445) pilB 920797..922503 (-) 1707 WP_151131894.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FXN65_RS04450 (FXN65_04450) - 922763..923263 (+) 501 WP_151131895.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FXN65_RS04455 (FXN65_04455) - 923348..925003 (+) 1656 WP_151131896.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43732.46 Da        Isoelectric Point: 9.5175

>NTDB_id=382797 FXN65_RS04440 WP_151131893.1 919577..920794(-) (pilC) [Metapseudomonas lalkuanensis strain PE08]
MAEKALKISTFAWEGTDKKGSRVKGEISGQNPALVKAQLRKQGINPIKVRKKSTSLLGAGKKIKPLDIALFTRQMATMMK
AGVPLMQSFDIIGEGFEKPAMRTLVNEVKQEVAAGNSLANSLRKKPDYFDELYCNLVDSGEQAGALETLLDRIATYKEKT
EALKAKIKKAMNYPIAVIVVAIIVSAILLIKVVPQFQSVFANFGAELPAFTLFVIGLSELLQEWWFIVLAGFFAAGFAIQ
QAHGKSEKFRDWLDHAVLKLPIVGDILYKSAVARFARTLATTFAAGVPLVDALDSVAGASGNVVFKNAVIKVKSDVSTGM
QLNFSMRTTGVFPAMAVQMTAIGEESGALDDMLDKVASFYEAEVDNAVDGLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=382797 FXN65_RS04440 WP_151131893.1 919577..920794(-) (pilC) [Metapseudomonas lalkuanensis strain PE08]
ATGGCGGAAAAAGCGCTGAAAATTAGTACTTTCGCATGGGAAGGTACGGACAAGAAAGGCTCCAGGGTCAAAGGCGAAAT
TTCGGGACAAAATCCCGCATTGGTGAAAGCGCAACTGCGCAAACAGGGTATCAACCCCATCAAGGTGCGTAAGAAGTCCA
CCTCGCTGCTGGGTGCAGGAAAGAAGATTAAGCCCTTAGATATCGCCCTGTTTACCCGACAGATGGCGACCATGATGAAA
GCCGGCGTTCCGCTGATGCAATCGTTCGACATTATCGGTGAAGGCTTTGAAAAGCCGGCGATGCGCACCCTGGTGAATGA
GGTCAAGCAGGAGGTTGCAGCAGGCAATAGCCTGGCCAACTCACTGCGCAAGAAGCCGGACTACTTCGACGAGCTCTACT
GCAACCTAGTGGACTCCGGCGAGCAGGCAGGCGCACTGGAAACCTTGCTGGACCGCATCGCCACCTACAAGGAAAAGACC
GAGGCACTAAAGGCCAAGATCAAGAAGGCGATGAACTATCCCATTGCAGTGATCGTGGTCGCCATCATTGTTTCGGCAAT
TCTGCTGATCAAAGTGGTTCCGCAGTTTCAATCGGTTTTCGCGAACTTTGGTGCCGAACTTCCTGCTTTTACACTTTTCG
TCATCGGGCTATCAGAGCTCCTGCAAGAATGGTGGTTTATCGTACTAGCCGGATTCTTCGCAGCAGGCTTCGCCATCCAG
CAAGCACATGGGAAATCGGAGAAGTTTCGCGATTGGCTTGACCACGCAGTACTGAAACTGCCAATCGTTGGCGACATCCT
CTACAAATCCGCCGTAGCCCGCTTTGCCCGCACTCTGGCAACCACCTTCGCCGCAGGTGTCCCCCTTGTGGATGCGTTGG
ACTCGGTAGCGGGCGCCAGTGGCAACGTGGTGTTCAAGAACGCGGTGATCAAGGTTAAGTCCGACGTCTCGACAGGCATG
CAACTGAACTTCTCCATGCGCACCACCGGCGTTTTCCCCGCCATGGCTGTCCAGATGACTGCCATCGGCGAAGAGTCCGG
CGCACTGGACGATATGCTCGACAAGGTCGCCAGCTTCTACGAAGCCGAAGTGGATAACGCCGTGGATGGCCTGACTGCAC
TCATGGAACCCATGATCATGGCAGTCCTCGGCGTGTTGGTCGGTGGCCTGATCATCGCCATGTACCTCCCCATCTTCCAA
CTCGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6QG59

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80.988

100

0.81
  pilC Acinetobacter baumannii D1279779

60.494

100

0.605
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Legionella pneumophila strain ERS1305867

52.141

98.025

0.511
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.926

100

0.459
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

40.148

100

0.402
  pilC Thermus thermophilus HB27

37.75

98.765

0.373

Multiple sequence alignment