Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FXN65_RS04445 Genome accession   NZ_CP043311
Coordinates   920797..922503 (-) Length   568 a.a.
NCBI ID   WP_151131894.1    Uniprot ID   A0A5J6QFH1
Organism   Metapseudomonas lalkuanensis strain PE08     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 915797..927503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXN65_RS04405 (FXN65_04405) - 915973..916395 (+) 423 WP_151131886.1 GNAT family N-acetyltransferase -
  FXN65_RS04410 (FXN65_04410) - 916400..916867 (+) 468 WP_151131887.1 FAD/FMN-containing dehydrogenase -
  FXN65_RS04415 (FXN65_04415) - 916864..917553 (+) 690 WP_151131888.1 energy-coupling factor ABC transporter permease -
  FXN65_RS04420 (FXN65_04420) - 917672..917887 (+) 216 WP_151131889.1 hypothetical protein -
  FXN65_RS04425 (FXN65_04425) yacG 917897..918097 (-) 201 WP_151131890.1 DNA gyrase inhibitor YacG -
  FXN65_RS04430 (FXN65_04430) coaE 918094..918705 (-) 612 WP_151131891.1 dephospho-CoA kinase -
  FXN65_RS04435 (FXN65_04435) pilD 918702..919574 (-) 873 WP_151131892.1 A24 family peptidase Machinery gene
  FXN65_RS04440 (FXN65_04440) pilC 919577..920794 (-) 1218 WP_151131893.1 type II secretion system F family protein Machinery gene
  FXN65_RS04445 (FXN65_04445) pilB 920797..922503 (-) 1707 WP_151131894.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FXN65_RS04450 (FXN65_04450) - 922763..923263 (+) 501 WP_151131895.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FXN65_RS04455 (FXN65_04455) - 923348..925003 (+) 1656 WP_151131896.1 PglL family O-oligosaccharyltransferase -
  FXN65_RS28135 - 925484..925882 (+) 399 WP_244620710.1 hypothetical protein -
  FXN65_RS28505 - 925858..926043 (+) 186 WP_394351273.1 IS3 family transposase -
  FXN65_RS04475 (FXN65_04475) nadC 926128..926976 (-) 849 WP_151131897.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62889.77 Da        Isoelectric Point: 5.5767

>NTDB_id=382798 FXN65_RS04445 WP_151131894.1 920797..922503(-) (pilB) [Metapseudomonas lalkuanensis strain PE08]
MTENAPTLSGLAKQLVQTSLLDERSALQAHTQAQRNKIPLITYLVQNKLLKSRDLAEMAAEQFGVAFLDLSSIDKESQPR
ELVSEKLIRQHRALPLWRRGNKLFVAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIEKFFDNASSGMEDLGDVDL
DSLDVEAVDDDKHDGANGTDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERSYRVRFRTDGMLHEVAKPPIQLASRIAA
RLKVMAALDISERRKPQDGRIKMKISKTKSIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKDLYLAALKQP
QGMILVTGPTGSGKTVSLYTGINILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCAHCKKEHEVPRETLLKEGF
PEDRIGSFKLYSPVGCENCNQGYKGRVGIYEVVKITSAMQQLIMEEGNSIQIAQQARRDGFNDLRTSGLVKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=382798 FXN65_RS04445 WP_151131894.1 920797..922503(-) (pilB) [Metapseudomonas lalkuanensis strain PE08]
ATGACCGAGAACGCTCCCACGCTATCTGGCCTGGCCAAACAATTGGTGCAGACCAGCCTCCTTGATGAGCGGAGCGCCCT
GCAGGCCCATACACAGGCCCAGCGCAACAAGATTCCGCTGATCACCTACCTCGTGCAGAACAAGCTGCTGAAAAGCCGCG
ACCTTGCAGAGATGGCGGCTGAGCAATTCGGGGTGGCCTTTCTCGATCTATCCAGCATTGACAAAGAGTCTCAGCCCAGG
GAACTGGTCAGCGAAAAGCTGATTCGCCAGCACCGCGCCCTACCACTGTGGCGCCGAGGTAACAAGCTGTTTGTTGCCAT
TTCCGATCCCACCAATCACCAGGCCGTTACCGACATCCAGTTCAGCACCGGGTTAAACACCGAGGCGATCCTCGTTGAAG
ATGACAAGCTGGGAGACGCCATCGAGAAGTTTTTCGATAACGCCTCCAGCGGCATGGAAGACTTAGGCGATGTCGACCTC
GACAGCTTGGACGTCGAGGCGGTCGATGATGATAAGCATGACGGCGCTAATGGTACCGACGCCGACGATGCGCCCGTCGT
GCGCTTCGTCAACAAGATGCTGCTGGACGCCATCCGCGGCGGCTCTTCAGACCTGCACTTCGAGCCCTACGAGCGCTCCT
ACCGGGTACGTTTCCGTACCGACGGCATGCTCCATGAAGTGGCCAAGCCCCCCATCCAGTTGGCCAGCCGCATCGCTGCC
CGCCTGAAGGTAATGGCCGCACTGGATATTTCCGAGCGGCGTAAACCCCAGGACGGGCGGATCAAGATGAAGATTTCCAA
GACGAAATCCATCGACTTCCGCGTCAATACCCTGCCCACGCTCTGGGGCGAGAAAATCGTGATGCGCATCCTCGACCCCA
CCAGTGCGCAGATGGGCATCGATGCGCTTGGCTACGAGGAAGAACAGAAAGACCTCTATCTGGCGGCCTTGAAGCAACCG
CAAGGCATGATCCTGGTGACCGGCCCCACTGGCTCGGGCAAGACAGTCTCCCTTTATACCGGCATCAATATCCTCAATAC
GGTGGACATCAATATTTCTACCGCTGAGGATCCAGTGGAAATCAACCTCGAGGGCATCAACCAGGTCAACGTCAACCCCA
AGCAGGGCATGGACTTCGCCCAGGCCCTGCGTGCCTTCCTGCGTCAGGACCCGGACGTGATCATGGTGGGTGAGATCCGC
GACCTGGAAACCGCCGAGATTGCCATCAAGGCCGCGCAGACCGGTCACATGGTGCTTTCAACCCTGCACACCAACAGCGC
CGCCGAAACCCTGACGCGCCTGCGCAATATGGGTGTGCCGGCGTTCAACATCGCCACCTCGGTGAACCTGATCATTGCCC
AGCGCCTGGCACGTAAATTGTGCGCCCACTGCAAGAAGGAGCATGAGGTCCCACGGGAAACCTTGCTCAAGGAAGGCTTC
CCGGAAGACCGGATTGGCAGCTTCAAGCTGTACAGCCCGGTGGGCTGCGAAAATTGCAACCAGGGCTACAAGGGCCGAGT
GGGTATTTATGAAGTGGTTAAAATCACGTCGGCCATGCAGCAGCTTATTATGGAAGAAGGCAACTCCATCCAGATCGCCC
AGCAGGCGCGCCGCGATGGCTTCAATGACCTGCGAACCTCGGGCCTGGTAAAAGCCATGAATGGCATCACCAGCCTTGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6QFH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.821

100

0.579
  pilB Acinetobacter baumannii D1279779

57.848

99.824

0.577
  pilB Legionella pneumophila strain ERS1305867

57.143

99.824

0.57
  pilB Vibrio cholerae strain A1552

50.714

98.592

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

51.941

95.246

0.495
  pilF Neisseria gonorrhoeae MS11

49.734

99.12

0.493
  pilB Vibrio campbellii strain DS40M4

48.511

100

0.488
  pilF Thermus thermophilus HB27

37.057

99.296

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

93.134

0.366

Multiple sequence alignment