Detailed information NTG0382796

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FXN65_RS04435
• Genome accession: NZ_CP043311
• Coordinates: 918702..919574 (-)
• Length: 290 a.a.
• NCBI ID: WP_151131892.1
• Uniprot ID: A0A5J6QIG1
• Organism: Metapseudomonas lalkuanensis strain PE08
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 913702..924574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FXN65_RS04395 (FXN65_04395)	-	914951..915124 (-)	174	WP_151131884.1	DUF3094 family protein	-
FXN65_RS04400 (FXN65_04400)	-	915213..915839 (-)	627	WP_151131885.1	DUF1780 domain-containing protein	-
FXN65_RS04405 (FXN65_04405)	-	915973..916395 (+)	423	WP_151131886.1	GNAT family N-acetyltransferase	-
FXN65_RS04410 (FXN65_04410)	-	916400..916867 (+)	468	WP_151131887.1	FAD/FMN-containing dehydrogenase	-
FXN65_RS04415 (FXN65_04415)	-	916864..917553 (+)	690	WP_151131888.1	energy-coupling factor ABC transporter permease	-
FXN65_RS04420 (FXN65_04420)	-	917672..917887 (+)	216	WP_151131889.1	hypothetical protein	-
FXN65_RS04425 (FXN65_04425)	yacG	917897..918097 (-)	201	WP_151131890.1	DNA gyrase inhibitor YacG	-
FXN65_RS04430 (FXN65_04430)	coaE	918094..918705 (-)	612	WP_151131891.1	dephospho-CoA kinase	-
FXN65_RS04435 (FXN65_04435)	pilD	918702..919574 (-)	873	WP_151131892.1	A24 family peptidase	Machinery gene
FXN65_RS04440 (FXN65_04440)	pilC	919577..920794 (-)	1218	WP_151131893.1	type II secretion system F family protein	Machinery gene
FXN65_RS04445 (FXN65_04445)	pilB	920797..922503 (-)	1707	WP_151131894.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FXN65_RS04450 (FXN65_04450)	-	922763..923263 (+)	501	WP_151131895.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31906.94 Da
• Isoelectric Point: 7.0714

>NTDB_id=382796 FXN65_RS04435 WP_151131892.1 918702..919574(-) (pilD) [Metapseudomonas lalkuanensis strain PE08]
MQLIDFLASHALAFVLCVLLLGLLVGSFLNVVIHRLPAMMMRDWQEQAREALSLPAEPKGPVFNLILPNSRCPHCSHEIR
AWENIPVVSYLALGGKCSSCKAPISKRYPLVELACGLLSAYVAWHFGFSWQTGAFLVLTWGLLAMSMIDVDHQLLPDALV
LPLLWLGLILNSQGLFTSLEDALWGAVAGYMSLWSVYWLFKLVTGKEGMGHGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AILGVIMLRLRNQDTATPIPFGPYLAIAGWIALLWGDQITATYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=382796 FXN65_RS04435 WP_151131892.1 918702..919574(-) (pilD) [Metapseudomonas lalkuanensis strain PE08]
ATGCAGTTGATTGACTTTCTGGCCAGCCACGCGCTGGCCTTTGTTTTATGCGTGCTCCTGCTGGGCTTGCTGGTGGGCAG
TTTCCTCAATGTGGTGATCCACCGCCTGCCGGCGATGATGATGCGCGACTGGCAGGAACAGGCCCGCGAGGCACTGAGCC
TGCCGGCCGAGCCGAAAGGGCCGGTGTTCAACCTCATCCTGCCGAACTCCCGCTGCCCCCATTGCAGCCACGAGATCCGC
GCCTGGGAAAACATCCCGGTAGTGAGCTATCTGGCGCTTGGCGGCAAGTGCTCTTCCTGCAAGGCGCCCATCAGCAAGCG
CTACCCCTTGGTGGAACTCGCCTGTGGCCTGCTGTCGGCCTACGTCGCCTGGCATTTCGGTTTCAGCTGGCAGACCGGCG
CCTTCCTGGTACTGACCTGGGGCCTGCTGGCGATGAGCATGATCGACGTCGATCACCAGTTGCTGCCGGACGCGCTGGTC
CTGCCGCTGCTCTGGCTCGGCCTGATCCTCAACAGCCAGGGCCTGTTCACCAGCCTGGAGGATGCGCTCTGGGGGGCGGT
GGCCGGCTATATGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCCACGGCGACT
TCAAATTGCTGGCGATGCTCGGCGCCTGGGGCGGCTGGCAGGTGCTGCCGCTGACCATCCTGCTGTCGTCCCTGGTGGGC
GCCATACTGGGTGTGATCATGCTGCGCCTGCGCAACCAGGACACCGCCACGCCCATTCCCTTTGGTCCTTACCTGGCGAT
TGCCGGCTGGATTGCGCTGCTCTGGGGTGATCAAATAACGGCGACCTATCTGCAGTTCGCCGGTTTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A5J6QIG1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.435	95.172	0.528
pilD	Vibrio campbellii strain DS40M4	53.846	94.138	0.507
pilD	Acinetobacter baumannii D1279779	50.694	99.31	0.503
pilD	Acinetobacter nosocomialis M2	50.347	99.31	0.5
pilD	Neisseria gonorrhoeae MS11	51.724	90	0.466