Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FLG15_RS16175 Genome accession   NZ_CP041380
Coordinates   3600688..3601116 (-) Length   142 a.a.
NCBI ID   WP_057677883.1    Uniprot ID   A0A1V9HE72
Organism   Xanthomonas phaseoli pv. dieffenbachiae strain D182     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3595688..3606116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLG15_RS16150 (FLG15_16160) pilR 3596406..3597860 (+) 1455 WP_170922314.1 sigma-54 dependent transcriptional regulator Regulator
  FLG15_RS16160 (FLG15_16170) - 3598459..3598578 (-) 120 WP_057687107.1 hypothetical protein -
  FLG15_RS16165 (FLG15_16175) - 3598640..3598759 (-) 120 WP_057687106.1 hypothetical protein -
  FLG15_RS16170 (FLG15_16180) pilB 3598821..3600530 (-) 1710 WP_057677884.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FLG15_RS16175 (FLG15_16185) pilA 3600688..3601116 (-) 429 WP_057677883.1 pilin Machinery gene
  FLG15_RS16180 (FLG15_16190) pilC 3601457..3602719 (+) 1263 WP_057677885.1 type II secretion system F family protein Machinery gene
  FLG15_RS16185 (FLG15_16195) - 3602726..3603589 (+) 864 WP_003491180.1 A24 family peptidase -
  FLG15_RS16190 (FLG15_16200) coaE 3603603..3604211 (+) 609 WP_022558665.1 dephospho-CoA kinase -
  FLG15_RS16195 (FLG15_16205) - 3604291..3605378 (+) 1088 WP_223842567.1 IS3-like element ISXcd1 family transposase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14369.47 Da        Isoelectric Point: 9.1206

>NTDB_id=371751 FLG15_RS16175 WP_057677883.1 3600688..3601116(-) (pilA) [Xanthomonas phaseoli pv. dieffenbachiae strain D182]
MKKQNGFTLIELMIVVAIIAILAAIALPAYQDYTVRGRVSEAMVAASAAKTVVAENAANGSALNSGWTPPTATNNVASVA
VAAATGNITVTTTDKAGGGTIIFAPTANGAALTSGTVPTDRISWDCKGGTLVAKYRPAECRT

Nucleotide


Download         Length: 429 bp        

>NTDB_id=371751 FLG15_RS16175 WP_057677883.1 3600688..3601116(-) (pilA) [Xanthomonas phaseoli pv. dieffenbachiae strain D182]
ATGAAGAAGCAAAACGGCTTTACCCTTATCGAACTGATGATCGTTGTCGCGATCATCGCAATCCTTGCTGCCATCGCGTT
GCCGGCCTACCAGGACTACACCGTTCGCGGCCGCGTCTCTGAAGCGATGGTTGCCGCGTCTGCCGCCAAGACGGTGGTGG
CCGAAAATGCCGCCAACGGCTCTGCCCTGAATAGCGGGTGGACCCCTCCTACGGCGACCAATAATGTTGCCAGCGTAGCC
GTTGCTGCCGCTACGGGCAATATTACTGTGACCACTACCGATAAGGCCGGCGGCGGCACGATCATTTTTGCTCCCACCGC
TAACGGCGCTGCGCTGACTTCCGGCACCGTTCCTACCGATCGCATTTCGTGGGATTGCAAGGGTGGCACCTTGGTTGCCA
AGTATCGTCCGGCTGAATGCCGCACCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V9HE72

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.572

100

0.577
  pilA2 Legionella pneumophila str. Paris

54.795

100

0.563
  pilA2 Legionella pneumophila strain ERS1305867

54.795

100

0.563
  comP Acinetobacter baylyi ADP1

48.428

100

0.542
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.894

100

0.528
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.789

100

0.437
  pilE Neisseria gonorrhoeae MS11

35.625

100

0.401
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.286

98.592

0.387
  pilA Acinetobacter baumannii strain A118

39.007

99.296

0.387

Multiple sequence alignment