Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FLG15_RS16170 Genome accession   NZ_CP041380
Coordinates   3598821..3600530 (-) Length   569 a.a.
NCBI ID   WP_057677884.1    Uniprot ID   -
Organism   Xanthomonas phaseoli pv. dieffenbachiae strain D182     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3593821..3605530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLG15_RS16145 (FLG15_16155) - 3594530..3596143 (+) 1614 WP_007965357.1 PAS domain-containing sensor histidine kinase -
  FLG15_RS16150 (FLG15_16160) pilR 3596406..3597860 (+) 1455 WP_170922314.1 sigma-54 dependent transcriptional regulator Regulator
  FLG15_RS16160 (FLG15_16170) - 3598459..3598578 (-) 120 WP_057687107.1 hypothetical protein -
  FLG15_RS16165 (FLG15_16175) - 3598640..3598759 (-) 120 WP_057687106.1 hypothetical protein -
  FLG15_RS16170 (FLG15_16180) pilB 3598821..3600530 (-) 1710 WP_057677884.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FLG15_RS16175 (FLG15_16185) pilA 3600688..3601116 (-) 429 WP_057677883.1 pilin Machinery gene
  FLG15_RS16180 (FLG15_16190) pilC 3601457..3602719 (+) 1263 WP_057677885.1 type II secretion system F family protein Machinery gene
  FLG15_RS16185 (FLG15_16195) - 3602726..3603589 (+) 864 WP_003491180.1 A24 family peptidase -
  FLG15_RS16190 (FLG15_16200) coaE 3603603..3604211 (+) 609 WP_022558665.1 dephospho-CoA kinase -
  FLG15_RS16195 (FLG15_16205) - 3604291..3605378 (+) 1088 WP_223842567.1 IS3-like element ISXcd1 family transposase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61685.58 Da        Isoelectric Point: 5.4503

>NTDB_id=371750 FLG15_RS16170 WP_057677884.1 3598821..3600530(-) (pilB) [Xanthomonas phaseoli pv. dieffenbachiae strain D182]
MGITGIARRLVQDGALDEASARTAMDQAAAAKIPLPQWFSEKKLVSASQLAAANAVEFGMPLLDVSAFDANQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGNDDEEMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDNALLAEGF
TPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKAANGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=371750 FLG15_RS16170 WP_057677884.1 3598821..3600530(-) (pilB) [Xanthomonas phaseoli pv. dieffenbachiae strain D182]
GTGGGAATTACAGGCATTGCACGCCGTTTGGTGCAGGATGGAGCACTGGATGAGGCAAGTGCCCGTACCGCAATGGACCA
AGCCGCGGCGGCGAAGATTCCGCTTCCCCAATGGTTCTCCGAGAAAAAGCTGGTTTCAGCGTCTCAATTGGCCGCTGCCA
ATGCAGTCGAGTTCGGCATGCCACTACTGGACGTGTCGGCATTCGACGCCAACCAAAACGCGGTGAAGCTGGTCAGCGAG
GAATTGCTCCAGAAGCATCAAGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCATTCTTGTCGACGAGGATCAGATCCGCC
GGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGCAACGACGACGAGGAGATGGGGGATCTG
GACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCGAAGGGTGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCGTCGGACATCCATTTCGAGCCGTATGAAGACGACT
ACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTGAACCAGCGCATCGCGGCG
CGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAAAAGTGGTGCTGCGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAGTTGTTCCTGGAGGCGATCCACAAGCCC
TATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTTATATACGGCGCTGGGAATTCTCAACGA
CGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTGGCGTCAACCAGGTGCAGCAGAACAACA
AGCGTGGCATGACCTTCGCGGCAGCCTTGCGCTCGTTCCTGCGACAGGATCCGGACATCATCATGGTCGGCGAAATCCGT
GACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGTGCTGTCGACGTTGCACACCAACGATGC
GCCGCAGACCATCGCCCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGGCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACCCTGCCTGATAACGCATTGCTGGCCGAAGGATTC
ACGCCTGCCCAGCTTGCCGCAGGGATAGAGCTGTATGAGGCGGTTGGCTGCGATGAGTGCACCGAAGGCTACAAGGGGCG
TACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGCTGGAAGGCGGCAATGCAATGCAGATCG
CCGAAGCCGCGCAGGCGATCGGTATCCGCGATTTGCGTCAGTCGGCGCTGATGAAGGCTGCGAATGGGGTGACCAGCCTG
GCAGAGATCAACCGGGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.69

99.824

0.566
  pilB Acinetobacter baylyi ADP1

55.458

99.824

0.554
  pilB Legionella pneumophila strain ERS1305867

53.076

100

0.531
  pilB Vibrio cholerae strain A1552

48.866

100

0.492
  pilF Neisseria gonorrhoeae MS11

49.296

99.824

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

98.418

0.462
  pilB Vibrio campbellii strain DS40M4

45.694

100

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

92.97

0.366
  pilF Thermus thermophilus HB27

39.423

91.388

0.36

Multiple sequence alignment