Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FE240_RS07465 Genome accession   NZ_CP040449
Coordinates   1586944..1588065 (-) Length   373 a.a.
NCBI ID   WP_193004001.1    Uniprot ID   A0A5J6WUR6
Organism   Aeromonas simiae strain A6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1581944..1593065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE240_RS07440 (FE240_07435) - 1582925..1583482 (-) 558 WP_193003997.1 TMEM165/GDT1 family protein -
  FE240_RS07445 (FE240_07440) - 1583727..1584056 (-) 330 WP_193003998.1 translation initiation factor -
  FE240_RS07450 (FE240_07445) - 1584177..1584404 (+) 228 WP_042047401.1 FeoA family protein -
  FE240_RS07455 (FE240_07450) feoB 1584401..1586671 (+) 2271 WP_193003999.1 Fe(2+) transporter permease subunit FeoB -
  FE240_RS07460 (FE240_07455) - 1586668..1586910 (+) 243 WP_193004000.1 FeoC-like transcriptional regulator -
  FE240_RS07465 (FE240_07460) pilU 1586944..1588065 (-) 1122 WP_193004001.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FE240_RS07470 (FE240_07465) - 1588124..1588477 (-) 354 WP_193004002.1 DMT family protein -
  FE240_RS07475 (FE240_07470) btuC 1588748..1589740 (+) 993 WP_193004488.1 vitamin B12 ABC transporter permease BtuC -
  FE240_RS07480 (FE240_07475) - 1589724..1590455 (+) 732 WP_193004003.1 ATP-binding cassette domain-containing protein -
  FE240_RS07485 (FE240_07480) - 1590461..1591525 (-) 1065 WP_193004004.1 3-deoxy-7-phosphoheptulonate synthase -
  FE240_RS07490 (FE240_07485) - 1591668..1592048 (+) 381 WP_193004005.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41440.59 Da        Isoelectric Point: 6.3562

>NTDB_id=364552 FE240_RS07465 WP_193004001.1 1586944..1588065(-) (pilU) [Aeromonas simiae strain A6]
MELQPLLRILALQDGSDLYLTTGAPPSAKFQGVLKQLGNEPLAPGEVAEVADSIMDEEQKLAFAKELEMNLALSIPKVGR
FRINIFKQRNEVSLVARNIKTEIPRFADLKLPPVLLDVIVEKRGLVLFIGGTGSGKSTSLAALIDHRNENSGGHIITIED
PVEYVHQHKKCIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALTFAETGHLAISTLHANNANQALDRI
INFFPEERRPQLLQDLGNNLKGFISQRLVKTKEGGRRAAVEVMLGTHTIRDMIKRGEFGSLKEVMEKSRALGMITFDEAL
FELVVEGAIEEEEAIKNADSANNLRLKVKLWREKGQIGNALDSTSWSLDIGDH

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=364552 FE240_RS07465 WP_193004001.1 1586944..1588065(-) (pilU) [Aeromonas simiae strain A6]
ATGGAATTGCAACCCCTGTTGCGCATCCTGGCTCTACAGGATGGTTCGGATCTTTATCTCACCACCGGCGCGCCACCGAG
CGCCAAATTCCAAGGCGTGCTCAAGCAGCTCGGCAATGAACCACTGGCCCCCGGCGAGGTCGCCGAGGTTGCCGACAGCA
TCATGGATGAAGAGCAGAAACTGGCCTTTGCCAAGGAGCTGGAGATGAACCTAGCCCTCTCCATCCCCAAGGTGGGACGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAGGTCTCGCTGGTCGCGCGCAACATCAAGACCGAGATCCCCCGTTTTGC
CGATCTCAAGCTGCCCCCCGTGCTGCTCGATGTCATCGTCGAGAAGCGCGGGCTGGTGCTGTTTATCGGCGGCACCGGCT
CGGGCAAGTCGACTTCACTGGCGGCCCTTATCGATCATCGCAATGAAAACAGCGGTGGCCACATCATCACCATCGAAGAT
CCGGTTGAATACGTGCACCAGCACAAGAAGTGCATCATCAACCAGCGCGAAGTCGGGGTCGACACCCGCAGCTTCCACGC
TGCGCTGAAAAACACCCTGCGCCAAGCACCGGACGTGATCCTCATCGGCGAGATCCGCGATCGCGAAACCATGGAGCACG
CCCTCACCTTCGCCGAGACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTCGATCGCATC
ATCAACTTCTTCCCCGAAGAGCGCCGCCCACAGCTGCTGCAAGACCTTGGCAACAACCTCAAGGGCTTCATCTCCCAGCG
CCTGGTCAAGACCAAGGAGGGGGGACGGCGCGCCGCCGTCGAGGTGATGCTCGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGCAGCCTCAAGGAAGTGATGGAGAAATCCCGCGCCCTTGGCATGATCACCTTCGACGAGGCGCTG
TTTGAGTTGGTGGTCGAGGGGGCCATCGAAGAGGAAGAGGCCATCAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GGTCAAGTTGTGGCGCGAGAAGGGGCAGATAGGCAACGCCCTCGACTCCACCTCCTGGTCGCTCGACATCGGCGACCACT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6WUR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.908

99.464

0.536
  pilU Acinetobacter baylyi ADP1

52.113

95.174

0.496
  pilU Vibrio cholerae strain A1552

51.558

94.638

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

90.885

0.378
  pilT Pseudomonas aeruginosa PAK

40.698

92.225

0.375
  pilT Legionella pneumophila strain Lp02

41.018

89.544

0.367
  pilT Legionella pneumophila strain ERS1305867

41.018

89.544

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.535

92.225

0.365
  pilT Acinetobacter baumannii strain A118

40.419

89.544

0.362
  pilT Acinetobacter baumannii D1279779

40.419

89.544

0.362
  pilT Acinetobacter nosocomialis M2

40.419

89.544

0.362

Multiple sequence alignment