Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLKF_RS11880 Genome accession   NC_013656
Coordinates   2413406..2414344 (-) Length   312 a.a.
NCBI ID   WP_012898624.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis KF147     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2408406..2419344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLKF_RS11840 (LLKF_2364) - 2409294..2410136 (-) 843 WP_012898618.1 metal ABC transporter substrate-binding protein -
  LLKF_RS11845 (LLKF_2365) - 2410133..2410570 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  LLKF_RS11850 (LLKF_2366) comGG 2410651..2410935 (-) 285 WP_012898619.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLKF_RS11855 (LLKF_2367) comGF 2410974..2411420 (-) 447 WP_038603761.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLKF_RS11860 (LLKF_2368) comGE 2411383..2411679 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLKF_RS11865 (LLKF_2369) comGD 2411651..2412082 (-) 432 WP_012898621.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLKF_RS11870 (LLKF_2370) comGC 2412042..2412425 (-) 384 WP_012898622.1 competence type IV pilus major pilin ComGC Machinery gene
  LLKF_RS11875 (LLKF_2371) comGB 2412439..2413512 (-) 1074 WP_012898623.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLKF_RS11880 (LLKF_2372) comGA 2413406..2414344 (-) 939 WP_012898624.1 competence type IV pilus ATPase ComGA Machinery gene
  LLKF_RS11885 - 2414614..2414796 (-) 183 WP_038603763.1 hypothetical protein -
  LLKF_RS11890 (LLKF_2373) - 2414787..2415542 (-) 756 WP_012898625.1 hypothetical protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35646.97 Da        Isoelectric Point: 6.6176

>NTDB_id=35928 LLKF_RS11880 WP_012898624.1 2413406..2414344(-) (comGA) [Lactococcus lactis subsp. lactis KF147]
MIPKMAKELIQKAIDRGASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFADPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHAASVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNKFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=35928 LLKF_RS11880 WP_012898624.1 2413406..2414344(-) (comGA) [Lactococcus lactis subsp. lactis KF147]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAAAGCAATTGATAGAGGTGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCTGATCCAGTAGGCTCGGGCAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCGCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACAAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAGAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.885

100

0.779
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.662

99.679

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA140

51.923

100

0.519
  comYA Streptococcus mutans UA159

51.923

100

0.519
  comYA Streptococcus gordonii str. Challis substr. CH1

51.438

100

0.516

Multiple sequence alignment