Detailed information NTG0035923

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLKF_RS11855
• Genome accession: NC_013656
• Coordinates: 2410974..2411420 (-)
• Length: 148 a.a.
• NCBI ID: WP_038603761.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis KF147
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2405974..2416420

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLKF_RS11820 (LLKF_2360)	-	2406092..2406469 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LLKF_RS11825 (LLKF_2361)	-	2406674..2407540 (+)	867	WP_012898615.1	RluA family pseudouridine synthase	-
LLKF_RS11830 (LLKF_2362)	-	2407578..2408387 (-)	810	WP_012898616.1	metal ABC transporter permease	-
LLKF_RS11835 (LLKF_2363)	-	2408380..2409117 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
LLKF_RS11840 (LLKF_2364)	-	2409294..2410136 (-)	843	WP_012898618.1	metal ABC transporter substrate-binding protein	-
LLKF_RS11845 (LLKF_2365)	-	2410133..2410570 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LLKF_RS11850 (LLKF_2366)	comGG	2410651..2410935 (-)	285	WP_012898619.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLKF_RS11855 (LLKF_2367)	comGF	2410974..2411420 (-)	447	WP_038603761.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLKF_RS11860 (LLKF_2368)	comGE	2411383..2411679 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLKF_RS11865 (LLKF_2369)	comGD	2411651..2412082 (-)	432	WP_012898621.1	competence type IV pilus minor pilin ComGD	Machinery gene
LLKF_RS11870 (LLKF_2370)	comGC	2412042..2412425 (-)	384	WP_012898622.1	competence type IV pilus major pilin ComGC	Machinery gene
LLKF_RS11875 (LLKF_2371)	comGB	2412439..2413512 (-)	1074	WP_012898623.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLKF_RS11880 (LLKF_2372)	comGA	2413406..2414344 (-)	939	WP_012898624.1	competence type IV pilus ATPase ComGA	Machinery gene
LLKF_RS11885	-	2414614..2414796 (-)	183	WP_038603763.1	hypothetical protein	-
LLKF_RS11890 (LLKF_2373)	-	2414787..2415542 (-)	756	WP_012898625.1	hypothetical protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.89 Da
• Isoelectric Point: 8.6749

>NTDB_id=35923 LLKF_RS11855 WP_038603761.1 2410974..2411420(-) (comGF) [Lactococcus lactis subsp. lactis KF147]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQLGISQSDSIKDWQIFCQQMRFELSGTKLDKVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=35923 LLKF_RS11855 WP_038603761.1 2410974..2411420(-) (comGF) [Lactococcus lactis subsp. lactis KF147]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTTATCTCAGGTTTAACAAAGATGTTGAAAGAACAATTGGGGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAAGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGAAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATCTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.936	95.27	0.466
comYF	Streptococcus mutans UA159	48.227	95.27	0.459
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385