Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CTCNB1_RS25165 Genome accession   NC_013446
Coordinates   5323345..5323779 (-) Length   144 a.a.
NCBI ID   WP_012840353.1    Uniprot ID   -
Organism   Comamonas thiooxydans     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5318345..5328779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTCNB1_RS24190 (CtCNB1_4755) - 5318425..5318898 (+) 474 WP_012840348.1 hypothetical protein -
  CTCNB1_RS24195 (CtCNB1_4756) - 5319008..5319358 (+) 351 WP_034348032.1 phage holin family protein -
  CTCNB1_RS24200 (CtCNB1_4757) - 5319317..5320963 (-) 1647 WP_041744702.1 M48 family metalloprotease -
  CTCNB1_RS24205 (CtCNB1_4758) moaC 5321164..5321634 (+) 471 WP_012840351.1 cyclic pyranopterin monophosphate synthase MoaC -
  CTCNB1_RS25160 (CtCNB1_4759) - 5321628..5323337 (-) 1710 WP_012840352.1 O-antigen ligase family protein -
  CTCNB1_RS25165 (CtCNB1_4760) pilA 5323345..5323779 (-) 435 WP_012840353.1 pilin Machinery gene
  CTCNB1_RS24220 (CtCNB1_4761) - 5323968..5324687 (-) 720 WP_012840354.1 FHA domain-containing protein -
  CTCNB1_RS24225 (CtCNB1_4762) ssb 5325004..5325552 (-) 549 WP_003064700.1 single-stranded DNA-binding protein Machinery gene
  CTCNB1_RS24230 (CtCNB1_4763) - 5325825..5326997 (-) 1173 WP_041744703.1 MFS transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14479.70 Da        Isoelectric Point: 9.5825

>NTDB_id=35570 CTCNB1_RS25165 WP_012840353.1 5323345..5323779(-) (pilA) [Comamonas thiooxydans]
MKRSIQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSEALVLASSAKTAVTENAATGKDLAAGIGAISATSNVAS
MTVASNTGVISVVTSSAAKSISLALTPTAGGSGLTSGTVPEGNISWKCSISDSTKAKYVPSECR

Nucleotide


Download         Length: 435 bp        

>NTDB_id=35570 CTCNB1_RS25165 WP_012840353.1 5323345..5323779(-) (pilA) [Comamonas thiooxydans]
ATGAAGCGTTCCATTCAAAAGGGTTTCACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGGTATTCTGGCTGCCGT
GGCTCTGCCTGCTTATCAGGATTACACAGTGCGTGCACGTGTTTCTGAAGCTCTGGTGTTGGCGTCTAGTGCTAAGACTG
CTGTGACGGAAAATGCCGCTACGGGTAAAGATCTCGCTGCTGGAATTGGAGCCATTTCTGCAACTAGTAATGTGGCTAGC
ATGACTGTCGCTTCTAATACTGGCGTTATTTCAGTTGTTACATCTTCGGCTGCCAAAAGCATCTCTTTGGCCCTGACTCC
TACTGCCGGAGGATCTGGCCTTACTTCTGGCACAGTTCCTGAAGGAAATATCAGCTGGAAATGCAGCATTTCTGACTCCA
CCAAGGCTAAGTACGTACCTTCTGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

48.765

100

0.549
  comP Acinetobacter baylyi ADP1

49.032

100

0.528
  pilA2 Legionella pneumophila str. Paris

50

100

0.5
  pilA2 Legionella pneumophila strain ERS1305867

49.306

100

0.493
  pilE Neisseria gonorrhoeae strain FA1090

38.095

100

0.444
  pilE Neisseria gonorrhoeae MS11

38.75

100

0.431
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.961

100

0.417
  pilA Pseudomonas aeruginosa PAK

38.411

100

0.403
  pilA/pilA1 Eikenella corrodens VA1

31.977

100

0.382
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.415

100

0.382

Multiple sequence alignment