Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Pstu14405_RS16745 Genome accession   NZ_CP036186
Coordinates   3642389..3642889 (+) Length   166 a.a.
NCBI ID   WP_003281200.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain ATCC 14405     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3637389..3647889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Pstu14405_RS16725 (Pstu14405_16815) coaE 3637519..3638127 (-) 609 WP_003281195.1 dephospho-CoA kinase -
  Pstu14405_RS16730 (Pstu14405_16820) pilD 3638285..3639154 (-) 870 WP_003281196.1 prepilin peptidase Machinery gene
  Pstu14405_RS16735 (Pstu14405_16825) pilC 3639157..3640374 (-) 1218 WP_003281197.1 type II secretion system F family protein Machinery gene
  Pstu14405_RS16740 (Pstu14405_16830) pilB 3640376..3642079 (-) 1704 WP_003281199.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Pstu14405_RS16745 (Pstu14405_16835) pilA 3642389..3642889 (+) 501 WP_003281200.1 pilin Machinery gene
  Pstu14405_RS16750 (Pstu14405_16840) - 3643392..3643946 (+) 555 WP_003281202.1 REP-associated tyrosine transposase -
  Pstu14405_RS16755 (Pstu14405_16845) cysN 3644189..3646087 (-) 1899 WP_003281204.1 sulfate adenylyltransferase subunit CysN -
  Pstu14405_RS16760 (Pstu14405_16850) cysD 3646099..3647016 (-) 918 WP_003281205.1 sulfate adenylyltransferase subunit CysD -

Sequence

Protein


Download         Length: 166 a.a.        Molecular weight: 16581.81 Da        Isoelectric Point: 4.3718

>NTDB_id=347416 Pstu14405_RS16745 WP_003281200.1 3642389..3642889(+) (pilA) [Stutzerimonas stutzeri strain ATCC 14405]
MKAQMQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVTEGLSLAGSAKAAVSENAANGAPFNSGWTAPGATSNVES
IAIDETTGAITITYDAAIDGGATLILTPVDGEAATGTALAGTDTASTIPSAGSITWLCASTGSTNANAALQKGSIKDKYV
PAVCRT

Nucleotide


Download         Length: 501 bp        

>NTDB_id=347416 Pstu14405_RS16745 WP_003281200.1 3642389..3642889(+) (pilA) [Stutzerimonas stutzeri strain ATCC 14405]
ATGAAAGCTCAGATGCAGAAGGGTTTTACCCTTATTGAATTGATGATCGTTGTGGCGATTATCGGGATTTTGGCTGCGGT
GGCGTTGCCGGCGTATCAGGATTACACCGTACGGGCAAAGGTGACTGAAGGTCTGTCCCTCGCTGGCTCTGCTAAAGCGG
CCGTTTCTGAAAATGCGGCAAACGGTGCTCCGTTCAACTCCGGATGGACCGCCCCCGGGGCCACTAGCAACGTTGAAAGC
ATTGCCATTGACGAGACCACGGGCGCTATCACAATTACTTATGATGCAGCAATTGACGGCGGCGCGACCTTAATCCTTAC
TCCAGTTGATGGTGAGGCTGCGACTGGCACCGCATTGGCTGGCACCGACACAGCATCCACTATCCCTTCGGCAGGCTCGA
TCACTTGGCTATGCGCGTCGACCGGCAGCACCAACGCTAATGCGGCTCTGCAAAAAGGCAGCATCAAAGACAAATACGTT
CCGGCGGTATGCCGCACGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-165)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50.581

100

0.524
  pilA2 Legionella pneumophila strain ERS1305867

52.121

99.398

0.518
  pilA2 Legionella pneumophila str. Paris

51.515

99.398

0.512
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.776

100

0.458
  comP Acinetobacter baylyi ADP1

40

100

0.422
  pilA/pilA1 Eikenella corrodens VA1

37.433

100

0.422
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

99.398

0.398
  pilE Neisseria gonorrhoeae MS11

37.572

100

0.392
  pilE Neisseria gonorrhoeae strain FA1090

36.31

100

0.367

Multiple sequence alignment