Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Pstu14405_RS16740 Genome accession   NZ_CP036186
Coordinates   3640376..3642079 (-) Length   567 a.a.
NCBI ID   WP_003281199.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain ATCC 14405     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3635376..3647079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Pstu14405_RS16710 (Pstu14405_16800) - 3635512..3636549 (+) 1038 WP_003281190.1 NADP(H)-dependent aldo-keto reductase -
  Pstu14405_RS16715 (Pstu14405_16805) - 3636617..3637297 (+) 681 WP_003281192.1 energy-coupling factor ABC transporter permease -
  Pstu14405_RS16720 (Pstu14405_16810) yacG 3637328..3637522 (-) 195 WP_003281194.1 DNA gyrase inhibitor YacG -
  Pstu14405_RS16725 (Pstu14405_16815) coaE 3637519..3638127 (-) 609 WP_003281195.1 dephospho-CoA kinase -
  Pstu14405_RS16730 (Pstu14405_16820) pilD 3638285..3639154 (-) 870 WP_003281196.1 prepilin peptidase Machinery gene
  Pstu14405_RS16735 (Pstu14405_16825) pilC 3639157..3640374 (-) 1218 WP_003281197.1 type II secretion system F family protein Machinery gene
  Pstu14405_RS16740 (Pstu14405_16830) pilB 3640376..3642079 (-) 1704 WP_003281199.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Pstu14405_RS16745 (Pstu14405_16835) pilA 3642389..3642889 (+) 501 WP_003281200.1 pilin Machinery gene
  Pstu14405_RS16750 (Pstu14405_16840) - 3643392..3643946 (+) 555 WP_003281202.1 REP-associated tyrosine transposase -
  Pstu14405_RS16755 (Pstu14405_16845) cysN 3644189..3646087 (-) 1899 WP_003281204.1 sulfate adenylyltransferase subunit CysN -
  Pstu14405_RS16760 (Pstu14405_16850) cysD 3646099..3647016 (-) 918 WP_003281205.1 sulfate adenylyltransferase subunit CysD -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61958.01 Da        Isoelectric Point: 5.2552

>NTDB_id=347415 Pstu14405_RS16740 WP_003281199.1 3640376..3642079(-) (pilB) [Stutzerimonas stutzeri strain ATCC 14405]
MSENISLSGLARQMVVAGVLDEKTAQQAQAQAIRSKTPFVTYVVQNKLAKGRAIAELASDQFGIALLDLSALDKESLPKD
LISEKLARQHRVLPLSRRGTKLYVAISDPANHQSIADIQFSTGLSIESILVEDDKLGEAIDKLYESGTTGLEELGEVDLD
GVDVETVNEDGKAEEGGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPREALLEEGFP
ADKIGTFKIYGPIGCENCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRASALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=347415 Pstu14405_RS16740 WP_003281199.1 3640376..3642079(-) (pilB) [Stutzerimonas stutzeri strain ATCC 14405]
ATGAGCGAAAACATTTCCCTATCCGGGCTGGCACGGCAGATGGTCGTGGCCGGCGTGTTGGACGAAAAAACGGCCCAGCA
GGCGCAGGCCCAGGCGATTCGTAGCAAAACACCCTTTGTCACCTATGTAGTGCAGAACAAGTTGGCCAAAGGCCGCGCTA
TAGCTGAGCTTGCATCCGACCAGTTCGGTATCGCCTTGCTGGACTTGTCCGCGCTGGATAAAGAGTCGCTACCCAAGGAT
CTGATAAGCGAGAAGCTGGCGCGTCAGCACCGTGTCTTGCCGCTGTCCAGGCGCGGCACCAAGCTCTATGTGGCCATCTC
CGATCCGGCCAATCACCAGTCGATTGCCGATATCCAGTTCAGTACCGGGCTGAGCATCGAGTCGATCCTGGTGGAAGACG
ACAAGCTGGGCGAGGCGATCGACAAGCTTTACGAAAGCGGCACCACCGGGCTGGAAGAGCTGGGTGAGGTCGATCTGGAT
GGCGTGGATGTCGAGACCGTCAACGAGGATGGAAAAGCCGAGGAGGGCGGAGAGGCCGCTGACGACGCGCCGGTAGTGCG
CTTCGTCAATAAGATGCTGCTCGACGCCATTCGCGGTGGCTCCTCCGACTTGCACTTCGAGCCTTACGAAAAAGCCTACC
GTGTGCGGCTTCGTACGGATGGCATTCTGCATGAAGTCGCTCGCCCACCGATCCAACTGGCGCCCCGCATATCTGCACGC
TTGAAGGTGATGGCCGGGCTGGATATCTCCGAGCGCCGCAAGCCGCAGGACGGGCGGATCAAGATGAAGCTGTCCAAGAC
CAAGGCCATCGACTTTCGGGTGAACACGCTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGAATCCTTGATCCTTCAA
GTGCTCAAATGGGCATTGATGCGCTGGGTTACGAAGAGGACCAGAAAGAGTTGTACATGAATGCCCTGAAGCAACCTCAG
GGCATGATTTTGGTCACCGGCCCTACGGGATCGGGCAAGACGGTATCCCTTTACACCGGTCTCAATATTCTCAACACGGT
GGATGTGAACATCTCGACCGCCGAAGACCCTGTGGAGATCAACCTTGAGGGTATCAACCAGGTCAACGTCAACCCGAAGC
AGGGTATGGATTTCTCCCAGGCGCTACGCGCGTTTCTGCGTCAGGACCCGGACATCATCATGGTGGGTGAGATTCGCGAC
CTGGAAACAGCCGAAATCGCGATCAAGGCGGCGCAGACGGGCCATATGGTGATGTCGACGCTGCACACCAACAGCGCCGC
TGAAACCCTGACCCGTCTTCGCAATATGGGCGTGGCCGCATTCAATATTGCGACCTCGGTCAACCTGATCATTGCGCAGC
GACTGGCACGCAAACTTTGCAACAGTTGCAAAAAAGAAGTGGAGTTGCCTCGCGAGGCCCTGCTGGAAGAGGGTTTCCCT
GCCGATAAGATCGGAACCTTCAAAATTTACGGCCCGATAGGCTGTGAAAATTGCAAAGGCGGATACAAAGGCCGTGTTGG
TATTTATGAAGTGGTTAAAAACACGCCGGCATTGGCCAGGATTATCATGGAAGACGGCAACTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGATCTGCGTGCTTCGGCTCTTGTTAAAGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTGAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.752

99.471

0.594
  pilB Acinetobacter baylyi ADP1

59.397

99.471

0.591
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.55
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.494
  pilB Vibrio cholerae strain A1552

51.099

96.296

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

52.308

91.711

0.48
  pilF Neisseria gonorrhoeae MS11

51.154

91.711

0.469

Multiple sequence alignment