Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW56_RS05735 Genome accession   NZ_CP036132
Coordinates   1137191..1140706 (+) Length   1171 a.a.
NCBI ID   WP_215597296.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS823     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1132191..1145706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW56_RS05705 (EXW56_05825) - 1133236..1133730 (-) 495 WP_002201417.1 DUF6584 family protein -
  EXW56_RS05710 (EXW56_05830) cspA 1134007..1134210 (+) 204 WP_000218989.1 RNA chaperone/antiterminator CspA -
  EXW56_RS05715 (EXW56_05835) - 1134328..1134513 (-) 186 WP_002011281.1 hypothetical protein -
  EXW56_RS05720 (EXW56_05840) - 1134929..1135510 (+) 582 WP_215597295.1 competence protein ComK -
  EXW56_RS05725 (EXW56_05845) - 1135861..1136454 (+) 594 WP_002201415.1 TVP38/TMEM64 family protein -
  EXW56_RS05730 (EXW56_05850) lepB 1136510..1137073 (+) 564 WP_002158924.1 signal peptidase I -
  EXW56_RS05735 (EXW56_05855) addB 1137191..1140706 (+) 3516 WP_215597296.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW56_RS05740 (EXW56_05860) addA 1140703..1144428 (+) 3726 WP_215597297.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW56_RS05745 (EXW56_05865) - 1144452..1144730 (+) 279 WP_002201412.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW56_RS05750 (EXW56_05870) - 1144947..1145654 (+) 708 WP_002201411.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134359.49 Da        Isoelectric Point: 5.1797

>NTDB_id=347016 EXW56_RS05735 WP_215597296.1 1137191..1140706(+) (addB) [Bacillus mycoides strain JAS823]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDTHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGAEIYIDGFHSFSPQELEIVRGLMRLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYERIKQVAREEKIEVEKTIPLMEQPRFHSQALAHLEMHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVAAEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMTTGLEALQFANIPPSLDQVLVANIDHSRLSDIKATFIIGVNEGVIPAAPMDEGMLSDEERNVLTAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKETFITNDVNDLSRSEQISYVTTP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSALVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRMLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=347016 EXW56_RS05735 WP_215597296.1 1137191..1140706(+) (addB) [Bacillus mycoides strain JAS823]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTGAA
ACAACGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACACAGCAGGCGTTAATTGGGA
GCGAAGATGTAAGAGGTTCTATTCGGGCGCAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGG
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTCCATATGTTGCTTCGTAAAATTGTAGAATCTCGTAAAGATGGACT
ATCTGTGTTTCAAAAAGCAGCGGAGCAAAATGGCTTCTTTGAACATCTTGGTAGTATGATTGCAGAATTTAAACGTTACA
ATGTGACGCCGTCTAACGTATATGAAATGTGGCAACAATTAGATACGCATAGCAGTAGTGCAGAGCAAAAGCTACTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCAGACTCTGAGTATGTAAAAGGCGCGGAAATTTATATAGATGGGTTCCATTCAT
TTTCTCCTCAAGAGCTAGAAATTGTTAGAGGGCTAATGAGATTAGGAACTAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTGTTTTATGAAACGACGTTAACGTATGAAAGAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAGTTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAATGCATTACGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCGAGTGTGACAATTAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGAAGACTTGTGGCGGCTGAAGACTATCGTTACCGAGATATAGCAGT
ACTTCTTCGTAACGGAGAAAGTTATTACGACGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCAATGTCACATCATCCGCTAGTAGAATGTATTCGTTCGGCTTTAGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGTGTGAAAACAGAACTTTTATATCCACTAGACGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGAT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAAAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAGGCATTACGTATTCGTGCAGAAGAAAGCGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTGTCTATGTTTACTGATGTAATGACGACAGGTCTTGAGGCACTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAGTTGCTAATATTGATCATTCTAGATTATCAGATATTAAAGCGACGTTCATTATTGGCGTAAATGAAGGCG
TGATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAAATGTTCTCACCGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGGGCATCTGAGAAGTTATA
CATTTCCTGTCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAAACGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAACAACGCCA
GAAGTAACTTTGTCTTACGTCATGCAGCAACTGCAAACGTGGAAGCGTTACGGATTTGAAGGCAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTGTTATCGAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTACGCTCATTTCGCTCAGCACGGTTTATCGCTAAGAGAGCGCGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAACTATTCCATGCAGCGCTGAAGAGAATTGCAGACAAGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCACCTTTCTGCTTTAGTAATAGAAGAAATTGCACCATTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAGCAAAAACTACAACAAATCATTTTCCGTACATCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGCTTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGCGGGACTGGATCGCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAGGATGAGAGTGGT
ACGTTCCTTCGTATTATTGACTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGTAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGACGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTACTAGGAGATGCTGACGTTGTACGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAATCACTTGAAGATAA
CCAATTCCGTATGTTAAAAGATATGAAAGATAGCGAAGCAATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.063

100

0.492

Multiple sequence alignment