Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW33_RS18100 Genome accession   NZ_CP036014
Coordinates   3518381..3521896 (+) Length   1171 a.a.
NCBI ID   WP_000058646.1    Uniprot ID   -
Organism   Bacillus toyonensis strain BPN45/4     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3513381..3526896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW33_RS18065 (EXW33_18475) - 3513434..3514225 (-) 792 WP_000073825.1 phosphotransferase -
  EXW33_RS18070 (EXW33_18480) - 3514245..3514739 (-) 495 WP_000208488.1 DUF6584 family protein -
  EXW33_RS18075 (EXW33_18485) cspA 3515018..3515221 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  EXW33_RS18080 (EXW33_18490) - 3515359..3515544 (-) 186 WP_000391369.1 hypothetical protein -
  EXW33_RS18085 (EXW33_18495) - 3515835..3516416 (+) 582 WP_000444438.1 competence protein ComK -
  EXW33_RS18090 (EXW33_18505) - 3516925..3517518 (+) 594 WP_000347508.1 TVP38/TMEM64 family protein -
  EXW33_RS18095 (EXW33_18510) lepB 3517701..3518264 (+) 564 WP_000751914.1 signal peptidase I -
  EXW33_RS18100 (EXW33_18515) addB 3518381..3521896 (+) 3516 WP_000058646.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW33_RS18105 (EXW33_18520) addA 3521893..3525618 (+) 3726 WP_000572315.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW33_RS18110 (EXW33_18525) - 3525642..3525920 (+) 279 WP_000224035.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW33_RS18115 (EXW33_18530) gerPF 3526009..3526224 (-) 216 WP_001141566.1 spore germination protein GerPF -
  EXW33_RS18120 (EXW33_18535) - 3526267..3526653 (-) 387 WP_000902326.1 spore germination protein GerPE -
  EXW33_RS18125 (EXW33_18540) gerPD 3526669..3526863 (-) 195 WP_001052804.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134582.90 Da        Isoelectric Point: 5.1165

>NTDB_id=345892 EXW33_RS18100 WP_000058646.1 3518381..3521896(+) (addB) [Bacillus toyonensis strain BPN45/4]
MSLRLVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIESEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETMLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLETHYEARPNEKYYDEASVTISTAANL
RAEVEGVAREIRRLVADETYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEMMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDIEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
VSLSMFTDVMSTGLEALQFANIPPSLDQVLVANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREILGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVIGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTTNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVDGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=345892 EXW33_RS18100 WP_000058646.1 3518381..3521896(+) (addB) [Bacillus toyonensis strain BPN45/4]
ATGTCACTTCGATTGGTGATTGGTAGAGCGGGAAGTGGAAAGAGTACACTTTGTTTACGAGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGCAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGAAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCTTGGAAGGTGTTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTGCATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCTGAGCAAAAGTTATTAGCG
AATAAAGTATATGATTTACAGCTATTATATGATGATTTTGAACGTGCTTTAATCGGGAAATATTTAGATTCAGAAGACTA
TTTACAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAGTATGTAAATGGTGCTGAAATTTATATAGATGGGTTTCATTCAT
TTTCTCCGCAAGAACTTGAAATTGTAAGACAGCTCATGATTTGTGGAGCGAGAGTCACAATTACGTTAACGATAGATGAA
AAAACATTAGCGCAGCCAGTGAATGAACTGGATTTATTTTATGAAACGATGTTAACATATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGTTTTCATTCTCCGGCATTAGCTCATT
TAGAAACGCATTATGAAGCGCGTCCGAATGAAAAGTATTATGATGAAGCAAGTGTAACAATTAGTACAGCGGCGAATTTA
CGAGCTGAAGTAGAAGGGGTTGCTCGTGAAATTCGTAGACTTGTCGCGGATGAAACGTATCGTTATCGAGATATTGCAGT
ACTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATTCCACACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCATTAGTAGAGTGTATTCGTTCTGCCTTAGAAATTATTAGCGGAAATTGGCGTTAT
GACGCGGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGGAAAGAAATGATGCGTGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGTT
ACCGTTCACTTGACGATACAAACGGAATGATAACAGATATTGAACGTGAAATGGAAGAGAAAATAAACAGGCTGCGTGAT
GTTGTAAGAACGCCAGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCGGGAACAGTGATGCAAATGTGCGAAGCGGTTTA
CTTATTTTTAGAAGAACTTGACGTTCCAAAGAAGTTAGAAGAATTACGTATACGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCAACAGACCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTTGTAGAAATGCTTGGTGAGGAGAAG
GTGTCACTATCAATGTTCACGGACGTTATGTCGACAGGACTTGAGGCACTTCAATTCGCTAACATTCCGCCATCATTAGA
TCAAGTATTAGTTGCCAATATTGATCACTCCAGGTTATCAGATGTGAAAGCTACGTTTATTATCGGCGTAAATGAAGGAG
TTATTCCAGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGAAATTCTTGGTGCGGCAGGAATTGAACTC
GCGCCAACGACGAGACAAACTTTATTAGAAGAGCAGTTCGTTATGTATCAGATGGTAACGAGAGCGTCTGAGAAATTATA
TATTTCATGCCCACTTGCAGATGAAGAAGGAAAGACATTACTTGCATCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGACTCGTTTATTACGAATGACGTAAACGACTTATCGCGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTGACGTTATCTTATGTCATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATCTAGATTTTTGGTG
GGACGTCTATAACTTCTACGTCACTTCGGATGAATGGAAACAAAAAAGTAGTCGCGTGTTATCGAGTTTATTCTATCGAA
ATCGTGCGCAGAAACTAAGTACGGTAGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTTTCTCGTATGGAA
CTCTTCAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTAGATGC
ACCAGATATCGGTGAACTATTCCATGCAGCACTGAAGAGAATTGCAGACAGGCTATTACGCGAAAACCGCACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAAAGACAAATTTTA
TTAAGCTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
CGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAGGTGCCATTCGGTATGGGCGGTACGGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTTATCGGCCGTATTGACCGTGTGGATAAGGCTGAAGATGAAAACGGA
ACATTCCTTCGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTCGTTACTACAAATGCACAAACGTGGATGAAAAAAGGCCACGCAGCATCACCTGCTGGTG
TACTGTACTTTCATATTCATAACCCGATTGTTGAAATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGCCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGTTTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATTGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTCTGTCAGTTTGATGAATCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGACGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.403

100

0.494

Multiple sequence alignment