Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EXW33_RS18105 Genome accession   NZ_CP036014
Coordinates   3521893..3525618 (+) Length   1241 a.a.
NCBI ID   WP_000572315.1    Uniprot ID   -
Organism   Bacillus toyonensis strain BPN45/4     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3516893..3530618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW33_RS18090 (EXW33_18505) - 3516925..3517518 (+) 594 WP_000347508.1 TVP38/TMEM64 family protein -
  EXW33_RS18095 (EXW33_18510) lepB 3517701..3518264 (+) 564 WP_000751914.1 signal peptidase I -
  EXW33_RS18100 (EXW33_18515) addB 3518381..3521896 (+) 3516 WP_000058646.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW33_RS18105 (EXW33_18520) addA 3521893..3525618 (+) 3726 WP_000572315.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW33_RS18110 (EXW33_18525) - 3525642..3525920 (+) 279 WP_000224035.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW33_RS18115 (EXW33_18530) gerPF 3526009..3526224 (-) 216 WP_001141566.1 spore germination protein GerPF -
  EXW33_RS18120 (EXW33_18535) - 3526267..3526653 (-) 387 WP_000902326.1 spore germination protein GerPE -
  EXW33_RS18125 (EXW33_18540) gerPD 3526669..3526863 (-) 195 WP_001052804.1 spore germination protein GerPD -
  EXW33_RS18130 (EXW33_18545) gerPC 3526870..3527484 (-) 615 WP_001070753.1 spore germination protein GerPC -
  EXW33_RS18135 (EXW33_18550) gerPB 3527553..3527759 (-) 207 WP_001012514.1 spore germination protein GerPB -
  EXW33_RS18140 (EXW33_18555) gerPA 3527774..3527995 (-) 222 WP_001111187.1 spore germination protein GerPA -
  EXW33_RS18145 (EXW33_18560) - 3528095..3528271 (-) 177 WP_000462849.1 aspartyl-phosphate phosphatase Spo0E family protein -
  EXW33_RS18150 (EXW33_18565) - 3528518..3529417 (+) 900 WP_001213082.1 fumarylacetoacetate hydrolase family protein -
  EXW33_RS18155 (EXW33_18570) - 3529453..3529734 (-) 282 WP_000926856.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142726.86 Da        Isoelectric Point: 4.8190

>NTDB_id=345893 EXW33_RS18105 WP_000572315.1 3521893..3525618(+) (addA) [Bacillus toyonensis strain BPN45/4]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERMIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAALLGMLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVKLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVLEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHTVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGNSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=345893 EXW33_RS18105 WP_000572315.1 3521893..3525618(+) (addA) [Bacillus toyonensis strain BPN45/4]
ATGATAGAGAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAATGGACG
TGACATTTTAGTCGCAGCAGCAGCTGGATCGGGAAAAACAGCAGTATTAGTTGAACGTATGATTAAAAAGATTATTAATG
AGGAAAATCCAGTCGATGTCGATCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTACTAATTGATGAGCCAGGATCTCAACATGTAAGAAAACAGCTGAGCCTATTAAATAAAGC
ATCTATTTCAACGATCCATTCATTTTGTTTACAAGTAATTAGAGGGTATTACTACATGCTGGATGTTGATCCTCGTTTTC
GTATTGCAAATCAAACAGAAAATGAGTTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGCTATACGAGTGACCGTAGTGATGATGACTTACAAAGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACCTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAACATATT
CGTAAAGCAACCGAACTCGCAATGCTTCCTGACGGCCCGGCGCCTCGCGTTGAAACCCTGCAAGCGGATGCAGCTTTACT
TGGAATGTTATCATCAGCAGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTCGTAAAAGTCTTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGACGGTGAAATGAAGCCGTCCGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAGGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCGATTATTAAATTTGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTACCGCTTCCGACTAGCAG
AACCAGGCTTATTCTTAGGAAAGTATAAACGCTTCACTCAAGAAGGATCGGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGACGCTGACGCTGAATTAAAACTAGGCGCTAGTTATCCAGAAGGTGAAGATGTAGCGGCTGAATTATTATGCA
TTCAGCAAACGGAAGAAGAAGTGCTAGAGGGTGAAGAAGGTACGGAAGTCGAAAAGGCGCAACTGGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGAAAAACGGATAGTATGCGACCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCTATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCAG
TATATGCAGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCACTTGCAGCAGTACTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGGTCATTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAAGAACAAGAAC
TTCATGAAAAACTAGAATGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTATGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
GCGTGTATTATATGACCGCGCAAGGCAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGGACGGCGAGAGCTCTTGGTGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAGGGACTTGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAGTTCATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCAATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCTTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTCGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCTGGAGCGTCTTGCTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTGCCATTACAAAGTGAACGAAAAGAAGAAGTGTACGATAGGTTAATGTGGAAGTACGGGTATGAGG
AAGCAACCTCTCATCGTGCGAAGCAATCCGTTACAGAAATAAAGAGAAATTATCAGTCTGAAGAAGGCAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGATTAACGTACGCAGAGCGTGG
GACGGCAGTCCATACCGTTATGCAACATGTAGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAACAAATTGCTG
GAATGGTAAATAAGGAATTGTTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTTCCATTTACGATGATGCTATCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAATAGCGGGGAATCGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAAGAAGATG
GTATTACGCTAATCGATTTCAAAACAGATACGATTGAAGGTAAATTCCCAGGCGGATTTGAACAAGCAAAACCAATTTTA
GAAGATCGCTATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTGAATATTGAGGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.408

100

0.537

Multiple sequence alignment