Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   EXW58_RS10305 Genome accession   NZ_CP035953
Coordinates   2000324..2000662 (+) Length   112 a.a.
NCBI ID   WP_002171706.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN03/1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1989561..2000662 2000324..2000662 within 0


Gene organization within MGE regions


Location: 1989561..2000662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW58_RS10230 (EXW58_10355) - 1989561..1990525 (-) 965 Protein_1941 site-specific integrase -
  EXW58_RS28705 (EXW58_10365) - 1990522..1990749 (+) 228 Protein_1942 hypothetical protein -
  EXW58_RS10240 (EXW58_10370) - 1990752..1991906 (+) 1155 WP_215581534.1 BppU family phage baseplate upper protein -
  EXW58_RS29055 (EXW58_10375) - 1991923..1992555 (+) 633 WP_286675236.1 phage holin family protein -
  EXW58_RS10255 (EXW58_10380) - 1992902..1993480 (-) 579 WP_215581535.1 suppressor of fused domain protein -
  EXW58_RS10260 (EXW58_10385) - 1993519..1993890 (-) 372 WP_255835212.1 HNH/ENDO VII family nuclease -
  EXW58_RS10265 (EXW58_10390) - 1993963..1994397 (-) 435 Protein_1947 hypothetical protein -
  EXW58_RS10270 (EXW58_10395) - 1994580..1994867 (-) 288 WP_016104706.1 hypothetical protein -
  EXW58_RS10275 (EXW58_10400) - 1994880..1995227 (-) 348 WP_016104707.1 hypothetical protein -
  EXW58_RS10280 (EXW58_10405) - 1995600..1996355 (-) 756 WP_016104708.1 hypothetical protein -
  EXW58_RS10285 (EXW58_10410) - 1996465..1997112 (+) 648 WP_016104709.1 HD domain-containing protein -
  EXW58_RS10290 (EXW58_10415) - 1997109..1999004 (+) 1896 WP_016104710.1 ABC-F family ATP-binding cassette domain-containing protein -
  EXW58_RS10295 (EXW58_10420) - 1999081..1999812 (+) 732 WP_002158065.1 Bax inhibitor-1/YccA family protein -
  EXW58_RS10300 (EXW58_10425) - 1999909..2000162 (+) 254 Protein_1954 DUF4318 domain-containing protein -
  EXW58_RS10305 (EXW58_10430) ssb 2000324..2000662 (+) 339 WP_002171706.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 112 a.a.        Molecular weight: 12881.69 Da        Isoelectric Point: 9.0926

>NTDB_id=345268 EXW58_RS10305 WP_002171706.1 2000324..2000662(+) (ssb) [Bacillus mycoides strain BPN03/1]
MMNRVVLIGRLTKEPELYYTKQGVAYARICVAVNRGFRNSLGEQQVDFINCVVWRKSAENVTEYCTKGSLVGITGRIHTS
NYDDEQGKRIYRTEVVIESITFLERRREGASQ

Nucleotide


Download         Length: 339 bp        

>NTDB_id=345268 EXW58_RS10305 WP_002171706.1 2000324..2000662(+) (ssb) [Bacillus mycoides strain BPN03/1]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAACAAGGAGTCGCTTATGC
ACGAATATGTGTTGCGGTGAATAGAGGTTTTCGAAATAGTTTAGGTGAACAACAAGTCGATTTTATAAATTGTGTTGTAT
GGCGAAAATCGGCTGAAAATGTAACAGAATATTGTACGAAGGGCTCACTTGTCGGGATTACCGGGCGTATTCATACGAGT
AATTACGATGATGAACAAGGAAAGAGAATATATAGAACTGAAGTTGTAATTGAGAGCATTACATTTTTGGAGAGAAGGCG
GGAGGGTGCATCGCAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

51.261

100

0.545

  ssbA Bacillus subtilis subsp. subtilis str. 168

56.604

94.643

0.536

  ssbB Bacillus subtilis subsp. subtilis str. 168

49.558

100

0.5

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.636

98.214

0.429

  ssbB Lactococcus lactis subsp. cremoris KW2

44.762

93.75

0.42

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.909

98.214

0.402

  ssbB/cilA Streptococcus mitis NCTC 12261

40.909

98.214

0.402

  ssbB/cilA Streptococcus pneumoniae Rx1

40

98.214

0.393

  ssbB/cilA Streptococcus pneumoniae D39

40

98.214

0.393

  ssbB/cilA Streptococcus pneumoniae R6

40

98.214

0.393

  ssbB/cilA Streptococcus mitis SK321

40

98.214

0.393

  ssbA Streptococcus mutans UA159

40

98.214

0.393


Multiple sequence alignment