Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ET948_RS05505 Genome accession   NZ_CP035697
Coordinates   1186533..1188254 (+) Length   573 a.a.
NCBI ID   WP_078926717.1    Uniprot ID   A0A1T4QY24
Organism   Vibrio cincinnatiensis strain 2409-02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1181533..1193254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET948_RS05485 pilM 1183812..1184834 (+) 1023 WP_154115192.1 type IV pilus assembly protein PilM Machinery gene
  ET948_RS05490 pilN 1184824..1185417 (+) 594 WP_154115193.1 PilN domain-containing protein Machinery gene
  ET948_RS05495 pilO 1185410..1186000 (+) 591 WP_078926719.1 type 4a pilus biogenesis protein PilO Machinery gene
  ET948_RS05500 pilP 1185990..1186508 (+) 519 WP_078926718.1 pilus assembly protein PilP Machinery gene
  ET948_RS05505 pilQ 1186533..1188254 (+) 1722 WP_078926717.1 type IV pilus secretin PilQ Machinery gene
  ET948_RS05510 aroK 1188441..1188959 (+) 519 WP_078926716.1 shikimate kinase AroK -
  ET948_RS05515 aroB 1188987..1190078 (+) 1092 WP_154115194.1 3-dehydroquinate synthase -
  ET948_RS05520 - 1190100..1191563 (+) 1464 WP_154115195.1 AAA family ATPase -
  ET948_RS05525 - 1191633..1192454 (+) 822 WP_078926713.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ET948_RS05530 rpe 1192581..1193252 (+) 672 WP_078926712.1 ribulose-phosphate 3-epimerase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62783.13 Da        Isoelectric Point: 4.7265

>NTDB_id=343874 ET948_RS05505 WP_078926717.1 1186533..1188254(+) (pilQ) [Vibrio cincinnatiensis strain 2409-02]
MIKGLNSVVVRSLRYLCIGVWTLSAVSVVASELTGANQLKNIDFRLNKNKEAVLIVELASPSTVVDVQKKAQGLTIELLN
TEVADDQLYLFDVKDFASAVETIEVFRQEPSTLLEVSIQGAFKYDYSLRSQYLEVVITELKKEEPAPLPNLMEKQGKLIS
INFQDIPVRNVLQLIADYNQFNLVVSDSVKGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQL
EKARLAEELGDLRSDIIKVHFAKASDIAEMIGGSGTVNMLSERGSISIDERTNALLIRELPENIKVIREIIESLDIPVKQ
VQIEARIVTVNEGNLDELGIRWGMSSKNGSHTVGGSIEGNMNTIGLYEPEDGTEMINNFLNVNLGATAANASSIAFQVAK
LGSGTLLDLELSAMQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVAFKKAVLSLKVTPQITPDNRLVL
DLSVTQDRPGDVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSLTNTIDKVPLLGDLPFLGALFRRSYQQLGKS
ELLIFVTPQVVIQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=343874 ET948_RS05505 WP_078926717.1 1186533..1188254(+) (pilQ) [Vibrio cincinnatiensis strain 2409-02]
ATGATTAAAGGATTAAATTCGGTAGTGGTACGTAGTTTGCGTTACCTGTGTATAGGCGTGTGGACACTGTCGGCTGTCAG
CGTTGTTGCCAGTGAATTAACAGGAGCGAATCAATTAAAGAATATTGATTTTCGTTTAAATAAAAATAAAGAGGCGGTTT
TGATCGTCGAGTTGGCCTCTCCCTCAACGGTAGTGGACGTACAAAAAAAAGCTCAAGGATTAACGATTGAACTACTCAAT
ACGGAAGTTGCTGATGATCAGCTCTACTTATTCGATGTAAAAGATTTTGCTTCAGCGGTGGAAACGATTGAAGTGTTTCG
TCAAGAGCCAAGTACTTTGCTCGAAGTGAGTATTCAAGGGGCCTTTAAGTATGATTATTCTTTACGCAGCCAATATTTAG
AGGTGGTCATTACCGAACTAAAGAAGGAAGAACCCGCCCCCCTTCCTAATTTGATGGAAAAACAAGGTAAGCTTATCTCC
ATTAACTTCCAAGATATTCCTGTTCGAAATGTCTTACAGTTAATTGCGGATTACAACCAATTTAATTTAGTGGTTTCTGA
CTCAGTTAAAGGGAACTTAACCCTACGTTTGGATGGTGTTCCTTGGCAGCAGGTGCTTGATATTATTTTACAGGTCAAAG
GGCTTGATAAGCGCGTTGATGGAAACGTGATTCTCGTTGCACCGAAAGAGGAGTTGGATTTACGAGAAAAGCAACAACTC
GAGAAAGCGCGTTTAGCGGAAGAACTGGGTGATTTACGCTCGGACATCATCAAAGTACATTTTGCGAAAGCCTCGGATAT
TGCTGAGATGATAGGTGGAAGTGGAACTGTCAATATGTTATCAGAGCGTGGTTCTATTAGTATTGATGAGCGAACGAATG
CCCTCTTGATCCGCGAATTACCTGAAAACATTAAAGTGATTCGTGAAATCATTGAGTCACTGGATATTCCTGTCAAACAA
GTACAGATTGAAGCCAGAATTGTGACGGTGAATGAAGGCAACTTAGACGAATTAGGGATCCGGTGGGGGATGTCATCGAA
AAACGGCAGTCATACCGTGGGCGGTTCTATTGAAGGAAACATGAACACGATTGGCTTATATGAACCGGAAGATGGTACCG
AAATGATCAATAACTTCTTGAACGTTAACTTGGGGGCCACGGCTGCTAACGCCTCAAGCATTGCGTTTCAGGTCGCAAAA
TTGGGGTCAGGGACTTTGCTGGATCTTGAATTATCAGCGATGCAGCGCGAGTCTAAAGCTGAAATCATCTCGAGCCCACG
GTTGATCACCACCAATAAAAAACCCGCTTACATTGAGCAAGGTACGGAAATCCCCTACTTAGAATCCTCTTCTAGCGGGG
CAACGACGGTGGCATTTAAAAAAGCGGTTCTCAGTTTGAAAGTGACACCTCAAATCACCCCAGATAACCGTTTGGTACTG
GATTTAAGTGTCACACAGGATCGCCCCGGTGATGTGGTTAAAACCGGCACAGGGGAAGCGGTAGCGATTAATACTCAACG
GATTGGTACCCAAGTGTTAGTCAATAATGGAGAAACCGTTGTACTGGGTGGTATTTTCCAACATAGCTTGACTAATACCA
TAGATAAAGTGCCTTTACTGGGTGATCTCCCTTTTCTTGGAGCACTCTTTCGCCGTAGTTATCAGCAATTGGGTAAAAGT
GAATTGCTGATTTTTGTCACGCCTCAGGTGGTGATTCAATGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-320)

Bacterial type II and III secretion system protein (Secretin)

(413-571)

Secretin and TonB N terminus short domain (STN)

(181-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4QY24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.487

100

0.775
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.487

100

0.775
  pilQ Vibrio campbellii strain DS40M4

76.588

96.161

0.736
  pilQ Legionella pneumophila strain Lp02

42.308

95.288

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.308

95.288

0.403
  comQ Acinetobacter baylyi ADP1

38.66

100

0.393
  pilQ Pseudomonas aeruginosa PAK

39.818

95.986

0.382
  pilQ Acinetobacter baumannii strain A118

36.441

100

0.375
  pilQ Acinetobacter baumannii D1279779

36.441

100

0.375

Multiple sequence alignment