Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ET948_RS05485 Genome accession   NZ_CP035697
Coordinates   1183812..1184834 (+) Length   340 a.a.
NCBI ID   WP_154115192.1    Uniprot ID   -
Organism   Vibrio cincinnatiensis strain 2409-02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1178812..1189834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET948_RS05465 - 1179011..1179280 (-) 270 WP_078926725.1 DUF3624 domain-containing protein -
  ET948_RS05470 - 1179376..1180107 (-) 732 WP_154115190.1 glutathione peroxidase -
  ET948_RS05475 oxyR 1180253..1181143 (+) 891 WP_078926723.1 DNA-binding transcriptional regulator OxyR -
  ET948_RS05480 - 1181193..1183670 (-) 2478 WP_154115191.1 penicillin-binding protein 1A -
  ET948_RS05485 pilM 1183812..1184834 (+) 1023 WP_154115192.1 type IV pilus assembly protein PilM Machinery gene
  ET948_RS05490 pilN 1184824..1185417 (+) 594 WP_154115193.1 PilN domain-containing protein Machinery gene
  ET948_RS05495 pilO 1185410..1186000 (+) 591 WP_078926719.1 type 4a pilus biogenesis protein PilO Machinery gene
  ET948_RS05500 pilP 1185990..1186508 (+) 519 WP_078926718.1 pilus assembly protein PilP Machinery gene
  ET948_RS05505 pilQ 1186533..1188254 (+) 1722 WP_078926717.1 type IV pilus secretin PilQ Machinery gene
  ET948_RS05510 aroK 1188441..1188959 (+) 519 WP_078926716.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37908.17 Da        Isoelectric Point: 6.9695

>NTDB_id=343870 ET948_RS05485 WP_154115192.1 1183812..1184834(+) (pilM) [Vibrio cincinnatiensis strain 2409-02]
MGKSLVTGVDIGHHSIKAVVLKLSGDTYSLVSYKEIAVTGDIFADNYTLDYQKIVKKLKELKKSLPLFNRKVSIAVPDNS
VISKILQIESDLEPAEQEFAIYQAFSHQSPLPIDDLSLDFIKISDNVLGRSRSATYQVYATRREVVESRISASEKAGLTP
VLLDVQMHSLVRIWQQVSQIQQRTNWMLLDIGYSQSSLCLDFIDKAPFCKEIPLGTRWFNQTEQPEGSHDVASLDSSTDV
QAFNQEFVERLARQIQLFTSVHGTHSLAGIWLSGGGASISGLAEAISERLALPCERFTPLSIFSHKLNKRRRTLNEQQRF
STAAGLALRGIEWLESGHAA

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=343870 ET948_RS05485 WP_154115192.1 1183812..1184834(+) (pilM) [Vibrio cincinnatiensis strain 2409-02]
ATGGGTAAATCATTAGTTACAGGTGTTGATATTGGGCATCACAGCATTAAAGCTGTGGTTTTAAAGTTAAGTGGCGATAC
CTATTCGCTCGTTAGCTATAAAGAAATTGCCGTAACCGGCGACATTTTCGCCGATAACTACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTCAAAAAGAGCCTACCTTTGTTTAACCGCAAGGTCTCTATTGCCGTACCTGATAACTCG
GTCATCAGCAAAATATTGCAAATAGAAAGTGATCTTGAACCCGCTGAGCAAGAATTTGCTATTTATCAGGCGTTCTCTCA
TCAGTCACCATTACCGATTGACGATCTGAGTTTAGATTTCATTAAGATATCAGATAACGTACTGGGACGAAGTCGTAGTG
CAACGTATCAAGTCTATGCTACGCGCCGTGAAGTGGTGGAAAGCCGTATTTCTGCTTCTGAAAAGGCAGGCCTTACTCCA
GTACTTCTTGATGTACAGATGCACAGTTTAGTGCGTATCTGGCAGCAAGTGAGCCAAATTCAGCAGAGAACGAATTGGAT
GTTACTTGATATTGGCTATAGCCAAAGTTCTTTGTGCCTTGATTTCATCGATAAAGCCCCATTTTGTAAAGAGATACCAC
TGGGTACTCGTTGGTTTAACCAAACTGAGCAGCCAGAGGGAAGCCATGATGTTGCATCGTTGGACAGCTCAACCGATGTA
CAAGCCTTTAATCAAGAGTTTGTTGAACGATTGGCGCGGCAAATTCAGCTATTTACTTCCGTACATGGCACACACAGTTT
GGCGGGAATATGGCTTTCTGGAGGGGGAGCTTCTATCTCCGGATTAGCGGAGGCGATTAGTGAGCGACTCGCATTACCAT
GTGAGCGGTTTACACCGCTCTCTATCTTTAGTCATAAATTGAATAAGCGTCGGCGAACGTTAAATGAGCAGCAACGATTC
AGTACCGCTGCTGGGTTAGCGTTACGAGGTATTGAATGGTTGGAGAGTGGGCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

62.647

100

0.626
  pilM Vibrio campbellii strain DS40M4

48.529

100

0.485

Multiple sequence alignment