Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EWA65_RS10565 Genome accession   NZ_CP035688
Coordinates   2320722..2322437 (-) Length   571 a.a.
NCBI ID   WP_195707969.1    Uniprot ID   -
Organism   Vibrio metoecus strain 08-2459     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2315722..2327437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA65_RS10545 - 2316463..2317296 (-) 834 WP_000744677.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EWA65_RS10550 - 2317366..2318844 (-) 1479 WP_055029208.1 AAA family ATPase -
  EWA65_RS10555 aroB 2318898..2319983 (-) 1086 WP_195707968.1 3-dehydroquinate synthase -
  EWA65_RS10560 aroK 2320011..2320535 (-) 525 WP_000818614.1 shikimate kinase AroK -
  EWA65_RS10565 pilQ 2320722..2322437 (-) 1716 WP_195707969.1 type IV pilus secretin PilQ Machinery gene
  EWA65_RS10570 pilP 2322463..2322981 (-) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  EWA65_RS10575 pilO 2322971..2323561 (-) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA65_RS10580 pilN 2323554..2324132 (-) 579 WP_000903040.1 PilN domain-containing protein Machinery gene
  EWA65_RS10585 pilM 2324122..2325138 (-) 1017 WP_055027425.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62410.53 Da        Isoelectric Point: 4.7319

>NTDB_id=343705 EWA65_RS10565 WP_195707969.1 2320722..2322437(-) (pilQ) [Vibrio metoecus strain 08-2459]
MRNGLKTYVAQAWLTLWVGLVLCATSVAHSAESASTNQLQNIDFRVNKDKAAVLIVELASPSVVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVQSIEVFRKEPSTQLIATIDGEFKHDYSLKGKYLEVVISKLKADEKPQPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGSIESNLWQQGLLADDKFPVDQFLNVNLASTSANASSIAFQVAKLG
SDTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVSFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=343705 EWA65_RS10565 WP_195707969.1 2320722..2322437(-) (pilQ) [Vibrio metoecus strain 08-2459]
ATGAGAAATGGATTAAAAACTTATGTCGCACAGGCTTGGCTGACCTTATGGGTTGGTTTGGTGCTTTGTGCTACTTCCGT
AGCGCATAGCGCGGAGTCGGCTTCTACCAATCAACTGCAAAATATTGATTTTCGGGTCAATAAAGACAAAGCCGCAGTGC
TGATTGTGGAGTTGGCTTCCCCCTCTGTTGTCGTGGATGTTCAAAAAGTCCAAGAAGGGCTGAGTATTGAACTGCTAAAA
ACGGATGTGGCAGATGACAAACTTTACTTATTGGATGTAAAGGACTTTTCTACGCCAGTGCAAAGCATAGAAGTGTTCCG
CAAAGAACCCAGCACTCAATTGATTGCGACCATTGATGGTGAGTTTAAACATGACTACAGCTTGAAAGGAAAATATTTGG
AGGTGGTGATCAGTAAGCTTAAAGCGGATGAAAAACCACAGCCGAAAAGTATTCTAGAGAAAGAAGGTAAGCTCATTTCA
ATTAACTTCCAAGATATTCCAGTGCGTAACGTGTTGCAGCTGATTGCCGATTATAACGGCTTCAACTTAGTAGTATCGGA
CTCGGTGGTCGGTAACCTGACGTTACGCTTGGATGGGGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAGG
GCTTAGATAAGCGTGTTGATGGTAACGTTATTCTCATCGCACCCAAAGAAGAACTGGATTTACGAGAAAAGCAGGCCTTA
GAGAAAGCGCGTTTAGCGGAAGAGCTGGGTGATTTAAAATCCGAAATCATCAAAATCAATTTTGCTAAAGCTTCAGATAT
CGCCGCCATGATCGGGGGAGAAGGTAACGTTAACATGCTCTCTGAGCGTGGTTCGATCAGCATCGATGAGCGTACGAACT
CACTGTTGATCCGAGAGTTACCCGATAATATTGCCGTGATCCGTGAGATCATCGAATCGCTGGATATTCCGGTTAAGCAA
GTGCAAATTGAAGCACGTATCGTCACCGTCAAAGAAGGTAATTTGGATGAGCTGGGTGTGCGTTGGGGCGTAATGTCAAC
CAATGGCAGTCATTCGGTCGGCGGGAGTATTGAAAGTAACTTATGGCAACAAGGGCTTTTGGCTGACGATAAGTTTCCGG
TCGATCAGTTCTTAAACGTCAATTTGGCATCCACATCAGCGAACGCTTCCTCCATCGCTTTCCAAGTGGCTAAGCTAGGG
TCCGATACCTTGTTGGATTTGGAGCTCTCAGCACTCCAAAACGAGTCGAAAGCAGAAATCATTTCTAGCCCTCGTTTGAT
TACCACTAATAAACAACCAGCGTACATCGAGCAGGGTACGGAAATCCCATACCTTGAGTCATCATCGAGTGGAGCGTCTA
CCGTATCGTTTAAAAAAGCGGTATTGAGCTTGAAAGTTACCCCGCAAATTACGCCGGATAACCGCTTGGTGCTGGATTTG
AGTGTGACTCAAGACCGCCGCGGTGAAACGGTGAAAACCGGTACAGGGGAAGCGGTCTCGATCGACACTCAGCGTATTGG
CACACAAGTGTTAGTGAATAATGGAGAAACTGTCGTCTTAGGTGGCATTTTCCAACACAGCATCAATAATTCAGTGGATA
AGGTGCCATTGCTTGGAGACCTGCCGGTTTTGGGTGCGCTATTTAGACGAACGTACGAGCAAATGGGCAAAAGTGAGCTG
TTGATTTTCGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

95.271

100

0.953
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

95.271

100

0.953
  pilQ Vibrio campbellii strain DS40M4

78.909

96.322

0.76
  pilQ Legionella pneumophila strain Lp02

42.989

94.921

0.408
  pilQ Legionella pneumophila strain ERS1305867

42.989

94.921

0.408
  comQ Acinetobacter baylyi ADP1

39.792

100

0.403
  pilQ Acinetobacter baumannii D1279779

38.055

100

0.391
  pilQ Acinetobacter baumannii strain A118

38.055

100

0.391
  pilQ Pseudomonas aeruginosa PAK

39.13

96.673

0.378

Multiple sequence alignment