Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   EWA65_RS10580 Genome accession   NZ_CP035688
Coordinates   2323554..2324132 (-) Length   192 a.a.
NCBI ID   WP_000903040.1    Uniprot ID   -
Organism   Vibrio metoecus strain 08-2459     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2318554..2329132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA65_RS10555 aroB 2318898..2319983 (-) 1086 WP_195707968.1 3-dehydroquinate synthase -
  EWA65_RS10560 aroK 2320011..2320535 (-) 525 WP_000818614.1 shikimate kinase AroK -
  EWA65_RS10565 pilQ 2320722..2322437 (-) 1716 WP_195707969.1 type IV pilus secretin PilQ Machinery gene
  EWA65_RS10570 pilP 2322463..2322981 (-) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  EWA65_RS10575 pilO 2322971..2323561 (-) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA65_RS10580 pilN 2323554..2324132 (-) 579 WP_000903040.1 PilN domain-containing protein Machinery gene
  EWA65_RS10585 pilM 2324122..2325138 (-) 1017 WP_055027425.1 type IV pilus assembly protein PilM Machinery gene
  EWA65_RS10590 - 2325280..2327757 (+) 2478 WP_055027426.1 penicillin-binding protein 1A -
  EWA65_RS10595 oxyR 2327853..2328746 (-) 894 WP_055064342.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 21965.34 Da        Isoelectric Point: 9.9269

>NTDB_id=343708 EWA65_RS10580 WP_000903040.1 2323554..2324132(-) (pilN) [Vibrio metoecus strain 08-2459]
MLHNINLLPWRDERREAHKRRFLGLVILGVLLAVLMQFGAGEYLSGQVAKQQERIGYLKQHIFSLDQQIAKLKVTEEEHK
ALLTRLNVVEQLQQKRNKTTDFMNQMPLLIPEGVYVDKIKMNGHEIEITGISDSTARLATMLDNLEKSPKLSDVEMHEIV
SGNKRFGKQFQSFKVSFQFLRSDSTQGGTNNG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=343708 EWA65_RS10580 WP_000903040.1 2323554..2324132(-) (pilN) [Vibrio metoecus strain 08-2459]
ATGCTGCATAACATTAACTTATTGCCATGGCGAGATGAGCGGCGCGAAGCACATAAACGGCGCTTTTTGGGGTTAGTGAT
TTTAGGCGTGCTGTTGGCAGTATTGATGCAGTTTGGTGCTGGAGAATATTTAAGTGGTCAGGTCGCCAAACAACAAGAAC
GGATTGGTTATTTGAAACAACACATCTTTTCGTTGGATCAGCAAATCGCCAAACTCAAAGTGACGGAAGAGGAACACAAG
GCATTGTTGACTCGCCTCAATGTTGTTGAGCAGCTACAGCAAAAACGCAATAAAACCACGGACTTTATGAATCAGATGCC
GCTGCTGATCCCTGAAGGCGTGTACGTCGATAAGATCAAAATGAATGGGCATGAAATCGAGATCACGGGGATCAGTGACT
CTACAGCTCGCTTAGCGACCATGCTCGACAACTTAGAAAAATCCCCCAAGCTCAGTGACGTAGAAATGCATGAAATAGTC
TCGGGTAACAAACGCTTTGGTAAGCAATTTCAGAGCTTTAAAGTTTCCTTTCAATTCTTGAGGTCAGACTCGACACAAGG
TGGGACGAATAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

86.082

100

0.87
  pilN Vibrio campbellii strain DS40M4

58.333

100

0.583

Multiple sequence alignment