Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EWA65_RS10585 Genome accession   NZ_CP035688
Coordinates   2324122..2325138 (-) Length   338 a.a.
NCBI ID   WP_055027425.1    Uniprot ID   A0A0Q0M3H5
Organism   Vibrio metoecus strain 08-2459     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2319122..2330138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA65_RS10560 aroK 2320011..2320535 (-) 525 WP_000818614.1 shikimate kinase AroK -
  EWA65_RS10565 pilQ 2320722..2322437 (-) 1716 WP_195707969.1 type IV pilus secretin PilQ Machinery gene
  EWA65_RS10570 pilP 2322463..2322981 (-) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  EWA65_RS10575 pilO 2322971..2323561 (-) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA65_RS10580 pilN 2323554..2324132 (-) 579 WP_000903040.1 PilN domain-containing protein Machinery gene
  EWA65_RS10585 pilM 2324122..2325138 (-) 1017 WP_055027425.1 type IV pilus assembly protein PilM Machinery gene
  EWA65_RS10590 - 2325280..2327757 (+) 2478 WP_055027426.1 penicillin-binding protein 1A -
  EWA65_RS10595 oxyR 2327853..2328746 (-) 894 WP_055064342.1 DNA-binding transcriptional regulator OxyR -
  EWA65_RS10600 - 2328886..2329617 (+) 732 WP_055027427.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37649.46 Da        Isoelectric Point: 7.2629

>NTDB_id=343709 EWA65_RS10585 WP_055027425.1 2324122..2325138(-) (pilM) [Vibrio metoecus strain 08-2459]
MGKSLVTGIDIGHHSIKAVVLKPMGDTYALMGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSQKVAIAIPDNA
VISKVLQIDSDLELREQEFAIYQAFSHQSPFPVEDLSLDFVKVAEKNLARSSTTTFQVYATKKEVVESRLQASKKAGFEP
ILMDVQVHSLLHLWQLASRAYRRADWMLIDIGHTQSSLCLDFPDKMPFYKDVPLGARLVDLDSANTSMLNPPQLHDSTQR
FINELVDKVARQIQLFTSVHGTQSLSGLWLSGGGAAMEGLPEALSQRLSLPCEVLNPFALFKMQVSKRKRQVVDGQRFST
AAGLALRGLAWLESEHAA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=343709 EWA65_RS10585 WP_055027425.1 2324122..2325138(-) (pilM) [Vibrio metoecus strain 08-2459]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCCGTGGTGCTCAAACCTATGGGCGACAC
CTATGCGCTTATGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGGTTACCCCTATTTAGTCAGAAAGTCGCGATTGCAATCCCGGATAACGCG
GTAATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCTGCGAGAGCAGGAGTTCGCTATTTATCAGGCTTTTTCCCA
CCAATCTCCATTTCCCGTTGAAGATCTTAGCCTTGATTTCGTCAAAGTGGCGGAAAAAAATCTCGCACGCAGTAGTACGA
CGACGTTTCAAGTCTATGCCACCAAGAAAGAAGTGGTTGAAAGCCGCTTACAAGCCAGTAAAAAAGCAGGTTTTGAGCCG
ATCTTAATGGATGTGCAAGTGCACAGTTTGTTGCATCTATGGCAATTGGCTAGCCGCGCGTATCGCCGTGCGGACTGGAT
GCTGATTGATATTGGACACACACAAAGCTCTTTGTGTTTGGACTTTCCAGACAAAATGCCTTTTTACAAAGATGTGCCAC
TAGGAGCTCGATTGGTCGATTTAGACTCTGCCAACACATCCATGCTCAATCCTCCCCAATTGCACGACTCGACTCAACGT
TTTATCAATGAATTGGTGGATAAAGTGGCGCGTCAAATCCAACTGTTTACGTCGGTGCATGGAACGCAAAGCCTCAGTGG
ATTATGGCTATCAGGCGGTGGTGCGGCGATGGAGGGATTGCCAGAAGCGTTGTCCCAGCGTTTATCTTTGCCTTGTGAAG
TGCTGAATCCTTTTGCGTTGTTCAAAATGCAGGTCTCAAAACGTAAACGCCAAGTGGTTGATGGTCAGCGTTTTAGCACT
GCAGCAGGGCTCGCTTTGCGTGGTTTAGCATGGCTGGAGAGTGAACATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(240-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-200)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0M3H5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

85.207

100

0.852
  pilM Vibrio campbellii strain DS40M4

49.112

100

0.491

Multiple sequence alignment