Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EPB59_RS01450 Genome accession   NZ_CP035686
Coordinates   299695..300150 (-) Length   151 a.a.
NCBI ID   WP_154171386.1    Uniprot ID   -
Organism   Vibrio metoecus strain 2011V-1169     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 294695..305150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB59_RS01430 coaE 295181..295789 (-) 609 WP_195707027.1 dephospho-CoA kinase -
  EPB59_RS01435 pilD 295786..296661 (-) 876 WP_154171384.1 A24 family peptidase Machinery gene
  EPB59_RS01440 pilC 296719..297945 (-) 1227 WP_055051503.1 type II secretion system F family protein Machinery gene
  EPB59_RS01445 pilB 297999..299687 (-) 1689 WP_154171385.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPB59_RS01450 pilA 299695..300150 (-) 456 WP_154171386.1 pilin Machinery gene
  EPB59_RS01455 nadC 300395..301285 (-) 891 WP_055065163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EPB59_RS01460 ampD 301573..302124 (+) 552 WP_154171387.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EPB59_RS01465 fldB 302188..302706 (-) 519 WP_095467139.1 flavodoxin FldB -
  EPB59_RS01470 xerD 302874..303752 (+) 879 WP_162231908.1 site-specific tyrosine recombinase XerD -
  EPB59_RS01475 dsbC 303768..304520 (+) 753 WP_154171389.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15753.25 Da        Isoelectric Point: 9.6893

>NTDB_id=343639 EPB59_RS01450 WP_154171386.1 299695..300150(-) (pilA) [Vibrio metoecus strain 2011V-1169]
MKAYKNKQQKGFTLIELMIVVAVIGVLAAIAMPQYQKYVAKSEIASVLATLTGSKTNVEAYTVENGIFPKAGDETKLGVP
TMPLGVAVFPASPTNDGGTIAFTFTSVASGASSLTAGTNLTLTRTASTGAWKCSTEGSKKLDTELLPKNCK

Nucleotide


Download         Length: 456 bp        

>NTDB_id=343639 EPB59_RS01450 WP_154171386.1 299695..300150(-) (pilA) [Vibrio metoecus strain 2011V-1169]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTAATGATTGTGGTGGCTGTAATTGGTGTGTT
GGCAGCGATTGCAATGCCCCAATACCAGAAATATGTTGCTAAAAGTGAAATCGCCTCTGTTTTAGCAACACTGACTGGCA
GCAAGACCAACGTAGAGGCATATACTGTTGAAAATGGTATTTTCCCCAAAGCAGGTGATGAAACCAAGCTAGGTGTTCCT
ACAATGCCTTTAGGTGTTGCTGTATTCCCTGCGTCACCTACAAATGATGGTGGAACAATCGCATTTACTTTTACTTCTGT
AGCTAGTGGTGCTAGCAGTTTGACAGCAGGAACAAATTTAACCTTGACACGAACAGCGTCTACAGGTGCATGGAAATGTT
CAACTGAAGGTAGCAAAAAGTTAGATACTGAACTATTACCTAAAAACTGTAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

49.045

100

0.51
  pilA Vibrio cholerae strain A1552

49.045

100

0.51
  pilA Vibrio cholerae C6706

49.045

100

0.51
  pilA Pseudomonas aeruginosa PAK

47.097

100

0.483
  pilA Vibrio parahaemolyticus RIMD 2210633

45.865

88.079

0.404
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.667

99.338

0.384
  pilA Acinetobacter baumannii strain A118

39.161

94.702

0.371
  pilA/pilA1 Eikenella corrodens VA1

35.22

100

0.371

Multiple sequence alignment