Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EPB59_RS01435 Genome accession   NZ_CP035686
Coordinates   295786..296661 (-) Length   291 a.a.
NCBI ID   WP_154171384.1    Uniprot ID   -
Organism   Vibrio metoecus strain 2011V-1169     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 290786..301661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB59_RS01415 parC 291721..294006 (+) 2286 WP_154171380.1 DNA topoisomerase IV subunit A -
  EPB59_RS01420 yacG 294055..294252 (-) 198 WP_154171381.1 DNA gyrase inhibitor YacG -
  EPB59_RS01425 zapD 294409..295149 (-) 741 WP_154171382.1 cell division protein ZapD -
  EPB59_RS01430 coaE 295181..295789 (-) 609 WP_195707027.1 dephospho-CoA kinase -
  EPB59_RS01435 pilD 295786..296661 (-) 876 WP_154171384.1 A24 family peptidase Machinery gene
  EPB59_RS01440 pilC 296719..297945 (-) 1227 WP_055051503.1 type II secretion system F family protein Machinery gene
  EPB59_RS01445 pilB 297999..299687 (-) 1689 WP_154171385.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPB59_RS01450 pilA 299695..300150 (-) 456 WP_154171386.1 pilin Machinery gene
  EPB59_RS01455 nadC 300395..301285 (-) 891 WP_055065163.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32483.82 Da        Isoelectric Point: 7.6293

>NTDB_id=343636 EPB59_RS01435 WP_154171384.1 295786..296661(-) (pilD) [Vibrio metoecus strain 2011V-1169]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPVVSWLALRGKCSHCKAPISARYPFIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LMWGGIALALLGFSPVSLSDAVIGAMVGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTVWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=343636 EPB59_RS01435 WP_154171384.1 295786..296661(-) (pilD) [Vibrio metoecus strain 2011V-1169]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGCAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCCGAATGTGCGGCAAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACACCGATTCGAGTGATT
GATAATATTCCGGTAGTCAGTTGGCTGGCGCTGCGCGGCAAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTACCC
CTTCATTGAGCTACTTACTGCCCTCATGAGCTTGGTGATTGCCACTCACTTTCCATTCGGTGTATTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTGTTGATTGCGGCAACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACATTACCC
TTAATGTGGGGCGGTATTGCGCTGGCACTGCTTGGCTTTTCTCCCGTTTCACTGAGTGATGCGGTGATCGGAGCCATGGT
GGGTTATCTCTCGCTGTGGTCGATTTATTGGCTATTTAAACTGCTGACTGGCAAAGAAGGCATGGGCTATGGCGATTTCA
AATTGCTCGCCGCACTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCAGTCATCGTGCTGCTCTCATCCGTAGTCGGGGTT
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
CGGATGGTTTGCCTTACTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCGTTTGGGTAGGACAACCGCTATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

97.595

100

0.976
  pilD Vibrio campbellii strain DS40M4

75.694

98.969

0.749
  pilD Acinetobacter baumannii D1279779

54.044

93.471

0.505
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Neisseria gonorrhoeae MS11

44.792

98.969

0.443

Multiple sequence alignment