Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EPB59_RS01445 Genome accession   NZ_CP035686
Coordinates   297999..299687 (-) Length   562 a.a.
NCBI ID   WP_154171385.1    Uniprot ID   -
Organism   Vibrio metoecus strain 2011V-1169     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 292999..304687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB59_RS01420 yacG 294055..294252 (-) 198 WP_154171381.1 DNA gyrase inhibitor YacG -
  EPB59_RS01425 zapD 294409..295149 (-) 741 WP_154171382.1 cell division protein ZapD -
  EPB59_RS01430 coaE 295181..295789 (-) 609 WP_195707027.1 dephospho-CoA kinase -
  EPB59_RS01435 pilD 295786..296661 (-) 876 WP_154171384.1 A24 family peptidase Machinery gene
  EPB59_RS01440 pilC 296719..297945 (-) 1227 WP_055051503.1 type II secretion system F family protein Machinery gene
  EPB59_RS01445 pilB 297999..299687 (-) 1689 WP_154171385.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPB59_RS01450 pilA 299695..300150 (-) 456 WP_154171386.1 pilin Machinery gene
  EPB59_RS01455 nadC 300395..301285 (-) 891 WP_055065163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EPB59_RS01460 ampD 301573..302124 (+) 552 WP_154171387.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EPB59_RS01465 fldB 302188..302706 (-) 519 WP_095467139.1 flavodoxin FldB -
  EPB59_RS01470 xerD 302874..303752 (+) 879 WP_162231908.1 site-specific tyrosine recombinase XerD -
  EPB59_RS01475 dsbC 303768..304520 (+) 753 WP_154171389.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62033.17 Da        Isoelectric Point: 6.0863

>NTDB_id=343638 EPB59_RS01445 WP_154171385.1 297999..299687(-) (pilB) [Vibrio metoecus strain 2011V-1169]
MLTNLVAILRQAELISATQEQAVVIQVSASGTSVPEALLGLGIFHAQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGTLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVGDDELQSIEDLSQDESPVSRFINQVLLDAVRKGASDIHFEPYESQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKISVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRATIHQIERLAKTNGMQSLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=343638 EPB59_RS01445 WP_154171385.1 297999..299687(-) (pilB) [Vibrio metoecus strain 2011V-1169]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTATACAGGT
TAGCGCTTCGGGGACTTCGGTGCCCGAAGCGCTGCTTGGTTTAGGGATTTTTCACGCCCAAGAACTGACTGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGATCTTAGCCGCTACGACTACGCCCCCCTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATTACCCGCTACGATGCACTGCCGATTGCCAAGCAAGGCACTTTATTACTGCTCGCCGTCTCAGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGCTTTGCCACTGGGCTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCGATTCAAGGCGCCGCCAATCAAGGCAAAGAGATCAGCCAAGATGAGCTCGCCAAC
CTGGTTAAAGTCGGCGATGACGAGCTGCAATCGATCGAAGATCTCAGCCAAGATGAGTCCCCCGTCAGTCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGTAAAGGTGCCTCGGATATTCATTTTGAGCCTTATGAAAGCCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTGGAAACTCAGCAACCCGCCAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGATGGGCGAATTAAACTGCGCCTGAGCCGCGATACCGCGAT
TGATATGCGCGTTTCCACGCTTCCCACTTTATGGGGTGAAAAAATCGTTCTGCGTCTGCTCGATAGTAGCGCCGCCAATC
TGGATATTGATAAGCTCGGTTATAACCCGCAGCAAAAACAGCTCTACCTCAATGCTCTTAAAAGACCGCAAGGAATGATC
TTGATGACCGGCCCCACCGGCAGCGGTAAAACCGTTTCGCTCTATACAGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACTGCGGAAGATCCGGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTCCACACCAACTCGGCCGCTGAAACCGT
GATTCGTTTGGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCTCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCAGAAGCCATTATGCAACGTGCCACCATTCACCAAATTGAACGTTTAGCTAAAACCAATG
GCATGCAAAGCTTGCAAGAATCCGGCTTAGAAAAACTACGTGAGGGCATCACCAGCTTTGCCGAGCTACAACGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

97.865

100

0.979
  pilB Vibrio parahaemolyticus RIMD 2210633

73.665

100

0.737
  pilB Vibrio campbellii strain DS40M4

73.31

100

0.733
  pilB Legionella pneumophila strain ERS1305867

51.429

93.416

0.48
  pilB Acinetobacter baylyi ADP1

50.87

91.993

0.468
  pilB Acinetobacter baumannii D1279779

50.484

91.993

0.464
  pilF Neisseria gonorrhoeae MS11

45.552

100

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

37.417

100

0.402
  pilF Thermus thermophilus HB27

40.111

96.263

0.386
  pilB Haemophilus influenzae 86-028NP

38.899

93.772

0.365

Multiple sequence alignment