Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   VCM66_RS00235 Genome accession   NC_012578
Coordinates   46074..47189 (+) Length   371 a.a.
NCBI ID   WP_000654771.1    Uniprot ID   -
Organism   Vibrio cholerae M66-2     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 41074..52189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCM66_RS00215 (VCM66_0044) rsmB 41997..43277 (-) 1281 WP_001102807.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  VCM66_RS00220 (VCM66_0045) fmt 43333..44280 (-) 948 WP_000083539.1 methionyl-tRNA formyltransferase -
  VCM66_RS00225 (VCM66_0046) def 44298..44807 (-) 510 WP_000115010.1 peptide deformylase -
  VCM66_RS00230 (VCM66_0047) - 44947..46074 (+) 1128 WP_000113371.1 LysM peptidoglycan-binding domain-containing protein -
  VCM66_RS00235 (VCM66_0048) dprA 46074..47189 (+) 1116 WP_000654771.1 DNA-processing protein DprA Machinery gene
  VCM66_RS00240 (VCM66_0049) - 47192..47668 (+) 477 WP_000979769.1 DUF494 family protein -
  VCM66_RS00245 (VCM66_0050) - 47684..48250 (+) 567 WP_000087517.1 type I DNA topoisomerase -
  VCM66_RS00250 (VCM66_0051) - 48323..49456 (-) 1134 WP_001265395.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  VCM66_RS00255 (VCM66_0052) purE 49460..49945 (-) 486 WP_000215768.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  VCM66_RS00260 (VCM66_0054) - 50112..50669 (+) 558 WP_032479111.1 L-threonylcarbamoyladenylate synthase -
  VCM66_RS00265 (VCM66_0055) hemF 50688..51605 (+) 918 WP_000841188.1 oxygen-dependent coproporphyrinogen oxidase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40004.98 Da        Isoelectric Point: 7.1834

>NTDB_id=33809 VCM66_RS00235 WP_000654771.1 46074..47189(+) (dprA) [Vibrio cholerae M66-2]
MKDQDLAAWLALCFTPKLGSKTISHLLATRLPAQLQSFTPKQWLACGLKPEQLVFLTTQAAKQAEQCLQWRSAANNRYIV
TPHCPLYPRLLKEINSSPPVLFIEGIWEAVHDPAVAIVGSRNASVDGRQIARQFATELAQSGLVVTSGLALGIDGYAHDG
ALQAQGQTVAVLGSGLAQVYPKQHQGLAERIIAQGALVSEFAPHTPPKADHFPRRNRIISGLSLGVVVVEAAEKSGSLIT
ARYAAEQGREVFVVPGSIFNAASQGSNQLIRQGACLVQSVQQIHQELKNALTWSLSEQVPYQATLFSAVQGDEELPFPEL
LANVGIEATPIDILASRTQIPVQDIMMQLLELELLGHVVAVPGGYIRKGRG

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=33809 VCM66_RS00235 WP_000654771.1 46074..47189(+) (dprA) [Vibrio cholerae M66-2]
ATGAAAGATCAGGATTTAGCGGCATGGTTGGCGCTCTGTTTTACTCCTAAACTAGGCAGCAAAACCATTTCTCACCTGCT
TGCGACCCGTTTGCCAGCCCAGTTGCAAAGTTTTACGCCTAAGCAATGGTTGGCCTGCGGGCTTAAGCCCGAACAACTGG
TGTTTTTAACCACTCAAGCGGCTAAACAAGCCGAGCAGTGTTTGCAATGGCGATCAGCAGCCAATAACCGCTATATCGTC
ACTCCTCATTGCCCGCTTTACCCTCGTTTATTGAAAGAGATTAACTCATCGCCTCCCGTCCTGTTTATTGAAGGAATATG
GGAAGCGGTGCATGACCCTGCGGTGGCTATCGTCGGTAGCCGCAATGCCAGTGTTGATGGGCGGCAGATCGCTCGCCAGT
TTGCCACTGAGCTCGCGCAGTCGGGTTTAGTGGTCACCAGTGGTTTAGCGCTTGGTATTGATGGCTATGCGCACGATGGA
GCTTTGCAAGCACAAGGGCAAACCGTGGCAGTATTAGGCTCAGGGCTGGCGCAGGTTTACCCCAAACAGCATCAAGGATT
AGCGGAGCGAATCATCGCCCAAGGGGCCTTGGTTTCTGAGTTTGCCCCTCACACACCGCCTAAAGCCGATCACTTTCCGC
GCCGTAACCGAATTATCAGCGGCTTATCGTTGGGCGTTGTGGTGGTAGAAGCTGCGGAGAAAAGCGGCTCACTCATCACT
GCGCGCTACGCCGCTGAGCAAGGGCGTGAGGTCTTTGTGGTTCCCGGCTCGATTTTTAATGCCGCCAGCCAAGGTAGCAA
TCAATTGATTCGTCAAGGCGCTTGTTTGGTGCAAAGTGTGCAACAAATTCATCAAGAGCTCAAAAATGCGCTGACTTGGT
CACTCTCTGAACAAGTTCCTTATCAAGCAACACTTTTTTCTGCTGTACAGGGCGATGAAGAATTGCCATTTCCCGAGCTG
TTAGCTAACGTAGGAATAGAAGCTACACCTATTGATATTCTCGCAAGTCGGACCCAGATACCGGTGCAAGATATCATGAT
GCAGCTCTTGGAGCTTGAGCTCCTTGGGCATGTGGTTGCAGTACCTGGTGGCTATATTAGAAAGGGGAGAGGCTAG

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(319-364)

DNA recombination-mediator protein A (DNA_processg_A)

(77-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio cholerae strain A1552

99.73

100

0.997
  dprA Vibrio campbellii strain DS40M4

58.108

99.73

0.58
  dprA Glaesserella parasuis strain SC1401

43.617

100

0.442
  dprA Legionella pneumophila strain ERS1305867

43.316

100

0.437
  dprA Neisseria meningitidis MC58

37.845

100

0.407
  dprA Neisseria meningitidis strain C311

37.845

100

0.407
  dprA Neisseria gonorrhoeae strain FA1090

36.658

100

0.396
  dprA Acinetobacter baumannii D1279779

43.505

89.218

0.388
  dprA Neisseria gonorrhoeae MS11

35.84

100

0.385
  dprA Acinetobacter baumannii strain A118

43.202

89.218

0.385
  dprA Acinetobacter baylyi ADP1

41.742

89.757

0.375
  dprA Haemophilus influenzae Rd KW20

48.746

75.202

0.367

Multiple sequence alignment