Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EQK21_RS03775 Genome accession   NZ_CP035110
Coordinates   750892..751767 (+) Length   291 a.a.
NCBI ID   WP_128530403.1    Uniprot ID   -
Organism   Latilactobacillus curvatus strain SRCM103465     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 745892..756767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQK21_RS03745 (EQK21_03745) - 746120..747949 (+) 1830 WP_128530399.1 hypothetical protein -
  EQK21_RS03750 (EQK21_03750) - 748056..748535 (+) 480 WP_128530400.1 hypothetical protein -
  EQK21_RS03760 (EQK21_03760) - 748987..749364 (-) 378 WP_128530401.1 hypothetical protein -
  EQK21_RS03765 (EQK21_03765) - 749467..749967 (+) 501 WP_128530402.1 VanZ family protein -
  EQK21_RS03770 (EQK21_03770) - 750057..750788 (+) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  EQK21_RS03775 (EQK21_03775) comGA 750892..751767 (+) 876 WP_128530403.1 competence type IV pilus ATPase ComGA Machinery gene
  EQK21_RS03780 (EQK21_03780) comGB 751757..752506 (+) 750 WP_128530404.1 type II secretion system F family protein Machinery gene
  EQK21_RS03785 (EQK21_03785) comGB 752550..752756 (+) 207 WP_054644028.1 hypothetical protein Machinery gene
  EQK21_RS03790 (EQK21_03790) comGC 752753..753061 (+) 309 WP_004270901.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EQK21_RS03795 (EQK21_03795) - 753042..753485 (+) 444 WP_089556486.1 type II secretion system protein -
  EQK21_RS10080 - 753550..753777 (+) 228 WP_164882251.1 hypothetical protein -
  EQK21_RS03800 (EQK21_03800) - 753725..754228 (+) 504 WP_164882291.1 competence type IV pilus minor pilin ComGF -
  EQK21_RS03805 (EQK21_03805) - 754206..754529 (+) 324 WP_039099486.1 hypothetical protein -
  EQK21_RS03810 (EQK21_03810) - 754645..755664 (+) 1020 WP_338323057.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32052.88 Da        Isoelectric Point: 5.5654

>NTDB_id=334587 EQK21_RS03775 WP_128530403.1 750892..751767(+) (comGA) [Latilactobacillus curvatus strain SRCM103465]
MTEALTTLLQKCATEKAQDLYFRPLSTGWVIIERLATGLKPLQKVTLSEGNQWLNQMKYQAGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDPQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQVMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYAALLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVDGHNVAAHYELATAQQLEEAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=334587 EQK21_RS03775 WP_128530403.1 750892..751767(+) (comGA) [Latilactobacillus curvatus strain SRCM103465]
ATGACCGAAGCATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCAAC
GGGTTGGGTAATTATTGAGCGTTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGCGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACCGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTTGTTCGTTTCATTTA
TCCCATTGGGCAGACCTATCATTGCGACGACCCGCAGATTTTAAGTCGTTTAATGCAGTTAATTCAGCAACCAGGATTAT
TACTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAGTCATGCAAGATAAGATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCATTCTTACAGTTACAGGTGAATGAAGCCGCAGGGTTAAC
CTATGCAGCATTGTTGAAAATCAGTTTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCAGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCGCGAAGTACAACAGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGACGGCCACAATGTAGCAGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGAGGCTGTTCATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.892

95.876

0.67
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.382

93.471

0.405
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

43.019

91.065

0.392
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.852

92.784

0.388
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.852

92.784

0.388
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.852

92.784

0.388
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.852

92.784

0.388
  comGA Lactococcus lactis subsp. cremoris KW2

41.603

90.034

0.375
  comYA Streptococcus mutans UA140

37.113

100

0.371
  comYA Streptococcus mutans UA159

37.113

100

0.371
  comYA Streptococcus gordonii str. Challis substr. CH1

39.925

92.096

0.368

Multiple sequence alignment