Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EHC68_RS01850 Genome accession   NZ_CP034305
Coordinates   357006..358754 (+) Length   582 a.a.
NCBI ID   WP_031855784.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20151116002-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 352006..363754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHC68_RS01830 (EHC68_01855) pilM 354305..355321 (+) 1017 WP_031855783.1 type IV pilus biogenesis protein PilM Machinery gene
  EHC68_RS01835 (EHC68_01860) pilN 355305..355883 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  EHC68_RS01840 (EHC68_01865) pilO 355876..356469 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  EHC68_RS01845 (EHC68_01870) pilP 356459..356974 (+) 516 WP_015297395.1 pilus assembly protein PilP Machinery gene
  EHC68_RS01850 (EHC68_01875) pilQ 357006..358754 (+) 1749 WP_031855784.1 type IV pilus secretin PilQ Machinery gene
  EHC68_RS01855 (EHC68_01880) aroK 358943..359461 (+) 519 WP_005381319.1 shikimate kinase AroK -
  EHC68_RS01860 (EHC68_01885) aroB 359493..360593 (+) 1101 WP_025794806.1 3-dehydroquinate synthase -
  EHC68_RS01865 (EHC68_01890) - 360646..362163 (+) 1518 WP_025522123.1 SPOR domain-containing protein -
  EHC68_RS01870 (EHC68_01895) - 362233..363072 (+) 840 WP_005486862.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63708.82 Da        Isoelectric Point: 4.4093

>NTDB_id=330571 EHC68_RS01850 WP_031855784.1 357006..358754(+) (pilQ) [Vibrio parahaemolyticus strain 20151116002-3]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDTSTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTISEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGGDGEGEEGGDGVGIDDFLNVNLAATNPNA
ASIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNLLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=330571 EHC68_RS01850 WP_031855784.1 357006..358754(+) (pilQ) [Vibrio parahaemolyticus strain 20151116002-3]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTAGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGCATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGCATTGATTTAATG
AACACGTCGGTAAAAGACGAACAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTAGTGGAAAGTGTGGAAGTGTT
CCGCGATACCTCCACCACACGATTAGTTGCGTATATCGATGAAGAATATACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTCCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTGGTGGTTTC
GGATTCAGTCGAAGGCAACCTTACACTGCGTCTTGATGGTGTGCCGTGGCAACAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCTCCAACCGATGAGTTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGTGAGCTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATCAATGGTGAAGGCAACATTAGCATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAGCGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTCAAA
CAAGTACAAATTGAAGCCCGAATTGTCACCATCAGTGAGGGTAACATGGATGAGTTAGGTATTCGTTGGGGCTTCACATC
TATTAATGGTAATAACACCGTTGGTGGCTCTATTGAGAACAACCTAGCGACAATCGGTTTGTACGAAGGTGGTGGTGATG
GCGAAGGTGAAGAGGGTGGTGATGGTGTTGGTATCGATGATTTTCTTAATGTGAATTTAGCCGCGACCAACCCGAATGCG
GCGAGCATTGCGTTCCAAGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTGCAGCAAGAATC
AAAAGCAGAAATCATTTCGAGCCCACGATTGATCACGACCAATAAGAAGCCCGCGTACATTGAACAAGGTACCGAAATTC
CTTATCTAGAATCTTCATCGAGTGGTGCAACGTCAGTGACCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAG
ATCACACCTGACAATCTTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGCACAGG
AGAGGCAATGGCAATTGATACGCAGCGCATTGGTACCCAAGTTCTGGTGAATAATGGTGAGACGGTTGTGCTCGGTGGTA
TTTTTCAGCACAGCATTACTAACTCAGTGGATAAAGTGCCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGC
CGAAGTTACGAAAACGTTGGCAAAAGTGAGTTGCTAATTTTCGTTACGCCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.753

100

0.918
  pilQ Vibrio cholerae strain A1552

76.232

97.595

0.744
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.232

97.595

0.744
  pilQ Pseudomonas aeruginosa PAK

39.478

98.797

0.39
  pilQ Legionella pneumophila strain ERS1305867

41.016

94.674

0.388
  pilQ Legionella pneumophila strain Lp02

41.016

94.674

0.388
  pilQ Acinetobacter baumannii D1279779

37.625

100

0.387
  pilQ Acinetobacter baumannii strain A118

37.625

100

0.387
  comQ Acinetobacter baylyi ADP1

38.25

100

0.383

Multiple sequence alignment