Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FQU84_RS05660 Genome accession   NZ_CP042250
Coordinates   786940..787749 (+) Length   269 a.a.
NCBI ID   WP_097501533.1    Uniprot ID   -
Organism   Escherichia coli strain BCE049     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 781940..792749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FQU84_RS05650 (FQU84_05650) sslE 782214..786776 (+) 4563 WP_001034562.1 lipoprotein metalloprotease SslE -
  FQU84_RS05660 (FQU84_05660) pilD 786940..787749 (+) 810 WP_097501533.1 prepilin peptidase PppA Machinery gene
  FQU84_RS05665 (FQU84_05665) gspS2 787815..788225 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  FQU84_RS05670 (FQU84_05670) gspC 788243..789202 (+) 960 WP_001350730.1 type II secretion system protein GspC -
  FQU84_RS05675 (FQU84_05675) gspD 789232..791292 (+) 2061 WP_001350731.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29445.06 Da        Isoelectric Point: 8.3801

>NTDB_id=330568 FQU84_RS05660 WP_097501533.1 786940..787749(+) (pilD) [Escherichia coli strain BCE049]
MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGETPSTQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGLCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=330568 FQU84_RS05660 WP_097501533.1 786940..787749(+) (pilD) [Escherichia coli strain BCE049]
ATGCTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCATACTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAACGCCGAGTACGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGTCCGCATTGCCAGCAGACTATCCGCGTTCGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTTGAGTT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CGTGGCTGATTGCCGCGAGCATCATCGACCTCGACAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGGCGCAGCAGAGTCCGTTAACGCTACAAGACGCAGTCACCGGCGTTCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTCGCTG
CGTTAGGTGGATGGGTGGGGCCGTTGTCGTTACCCAATGTGGCTTTAATCGCATCATGCTGCGGTCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACTGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

40.659

100

0.413
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Neisseria gonorrhoeae MS11

38.911

95.539

0.372
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372

Multiple sequence alignment