Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EHC68_RS01830 Genome accession   NZ_CP034305
Coordinates   354305..355321 (+) Length   338 a.a.
NCBI ID   WP_031855783.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20151116002-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 349305..360321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHC68_RS01815 (EHC68_01840) - 349729..350457 (-) 729 WP_031855782.1 redoxin family protein -
  EHC68_RS01820 (EHC68_01845) oxyR 350628..351536 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  EHC68_RS01825 (EHC68_01850) - 351592..354162 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  EHC68_RS01830 (EHC68_01855) pilM 354305..355321 (+) 1017 WP_031855783.1 type IV pilus biogenesis protein PilM Machinery gene
  EHC68_RS01835 (EHC68_01860) pilN 355305..355883 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  EHC68_RS01840 (EHC68_01865) pilO 355876..356469 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  EHC68_RS01845 (EHC68_01870) pilP 356459..356974 (+) 516 WP_015297395.1 pilus assembly protein PilP Machinery gene
  EHC68_RS01850 (EHC68_01875) pilQ 357006..358754 (+) 1749 WP_031855784.1 type IV pilus secretin PilQ Machinery gene
  EHC68_RS01855 (EHC68_01880) aroK 358943..359461 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37789.40 Da        Isoelectric Point: 7.2506

>NTDB_id=330567 EHC68_RS01830 WP_031855783.1 354305..355321(+) (pilM) [Vibrio parahaemolyticus strain 20151116002-3]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIKQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEAMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=330567 EHC68_RS01830 WP_031855783.1 354305..355321(+) (pilM) [Vibrio parahaemolyticus strain 20151116002-3]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCAAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAATTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGCGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACTATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment