Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   SUB_RS00730 Genome accession   NC_012004
Coordinates   122871..123812 (+) Length   313 a.a.
NCBI ID   WP_012657673.1    Uniprot ID   -
Organism   Streptococcus uberis 0140J     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 117871..128812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SUB_RS00720 (SUB0116) rpoC 118674..122312 (+) 3639 WP_012657671.1 DNA-directed RNA polymerase subunit beta' -
  SUB_RS00725 (SUB0117) - 122433..122798 (+) 366 WP_041817508.1 DUF1033 family protein -
  SUB_RS00730 (SUB0118) comGA/cglA 122871..123812 (+) 942 WP_012657673.1 competence type IV pilus ATPase ComGA Machinery gene
  SUB_RS00735 (SUB0119) comYB 123724..124779 (+) 1056 WP_172631729.1 competence type IV pilus assembly protein ComGB Machinery gene
  SUB_RS00740 (SUB0120) comGC/cglC 124780..125103 (+) 324 WP_012657675.1 competence type IV pilus major pilin ComGC Machinery gene
  SUB_RS00745 (SUB0121) comGD 125057..125494 (+) 438 WP_012657676.1 competence type IV pilus minor pilin ComGD -
  SUB_RS00750 (SUB0122) comGE 125466..125765 (+) 300 WP_012657677.1 competence type IV pilus minor pilin ComGE -
  SUB_RS00755 (SUB0123) comGF 125725..126180 (+) 456 WP_012657678.1 competence type IV pilus minor pilin ComGF -
  SUB_RS00760 (SUB0124) comGG 126158..126517 (+) 360 WP_012657679.1 competence type IV pilus minor pilin ComGG -
  SUB_RS00765 (SUB0125) comYH 126562..127518 (+) 957 WP_012657680.1 class I SAM-dependent methyltransferase Machinery gene
  SUB_RS00770 (SUB0126) - 127574..128764 (+) 1191 WP_012657681.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35648.93 Da        Isoelectric Point: 6.7725

>NTDB_id=32904 SUB_RS00730 WP_012657673.1 122871..123812(+) (comGA/cglA) [Streptococcus uberis 0140J]
MIQSLGMQLIQEASLKQAQDIYLIPQKDSYQVFMRVGDSRQKIGDYEWKDATNLISHFKFVAGMNVGEKRRTQLGSCDYH
IDQQGLVSLRLSTVGDYRGHESLVIRLLATQESQLHYWFDSFERIEKAIHGRGLYLFAGPVGSGKTRLMYALMEKYMQDS
QIITIEDPVEIKQDAILQLQVNNSIGMTYDNLIKLSLRHRPDLVIIGEIRDTETAQAVIRASLTGAMVFSTIHAKSIPGV
FSRLIELGVKRDELLNCLNLIAYQRLIAGGGLIDFATEKFEEHTHNQWNQEVEQLLKQGYIDNEKAKLEKIKG

Nucleotide


Download         Length: 942 bp        

>NTDB_id=32904 SUB_RS00730 WP_012657673.1 122871..123812(+) (comGA/cglA) [Streptococcus uberis 0140J]
ATGATACAAAGCTTAGGCATGCAATTGATTCAAGAAGCCAGTCTCAAACAAGCACAAGATATTTATCTCATACCTCAAAA
AGACTCTTATCAAGTTTTTATGAGAGTTGGAGATAGTCGGCAAAAAATAGGAGATTATGAGTGGAAGGATGCGACAAATC
TCATTAGTCATTTTAAATTTGTCGCAGGTATGAATGTTGGCGAGAAAAGAAGAACACAACTAGGGTCCTGTGATTATCAC
ATAGATCAGCAAGGCCTTGTCTCCTTGAGACTGTCAACAGTGGGTGACTATCGTGGGCATGAAAGTTTGGTTATCCGCCT
TTTAGCCACACAAGAGTCACAATTACATTATTGGTTTGACAGTTTTGAAAGGATTGAAAAAGCCATTCATGGTCGAGGTC
TTTATTTATTTGCTGGTCCAGTAGGTTCAGGTAAAACACGGTTGATGTACGCGTTAATGGAAAAATATATGCAGGACAGC
CAAATTATTACGATTGAAGATCCTGTTGAGATTAAGCAAGATGCTATTTTACAACTCCAAGTCAATAATAGTATTGGAAT
GACTTATGATAACCTGATTAAATTGTCTTTGAGGCATCGGCCTGATTTAGTGATTATAGGTGAAATACGTGATACAGAAA
CAGCACAGGCTGTTATTAGAGCAAGTTTGACGGGGGCCATGGTTTTCTCGACCATTCATGCTAAGAGCATTCCAGGAGTT
TTCTCTCGACTGATTGAACTCGGTGTAAAACGAGATGAATTGTTAAACTGTCTCAATTTGATAGCTTATCAACGGTTAAT
TGCAGGAGGAGGGCTAATTGATTTTGCCACAGAAAAATTCGAAGAGCATACCCATAATCAATGGAATCAAGAGGTTGAGC
AACTTCTTAAACAAGGATATATCGATAACGAAAAAGCCAAGTTGGAAAAAATTAAAGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

63.023

99.361

0.626
  comYA Streptococcus mutans UA140

62.5

99.681

0.623
  comYA Streptococcus mutans UA159

62.5

99.681

0.623
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.058

99.361

0.617
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.415

99.361

0.61
  comYA Streptococcus gordonii str. Challis substr. CH1

58.654

99.681

0.585
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508

Multiple sequence alignment