Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   SUB_RS00735 Genome accession   NC_012004
Coordinates   123724..124779 (+) Length   351 a.a.
NCBI ID   WP_172631729.1    Uniprot ID   -
Organism   Streptococcus uberis 0140J     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 118724..129779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SUB_RS00725 (SUB0117) - 122433..122798 (+) 366 WP_041817508.1 DUF1033 family protein -
  SUB_RS00730 (SUB0118) comGA/cglA 122871..123812 (+) 942 WP_012657673.1 competence type IV pilus ATPase ComGA Machinery gene
  SUB_RS00735 (SUB0119) comYB 123724..124779 (+) 1056 WP_172631729.1 competence type IV pilus assembly protein ComGB Machinery gene
  SUB_RS00740 (SUB0120) comGC/cglC 124780..125103 (+) 324 WP_012657675.1 competence type IV pilus major pilin ComGC Machinery gene
  SUB_RS00745 (SUB0121) comGD 125057..125494 (+) 438 WP_012657676.1 competence type IV pilus minor pilin ComGD -
  SUB_RS00750 (SUB0122) comGE 125466..125765 (+) 300 WP_012657677.1 competence type IV pilus minor pilin ComGE -
  SUB_RS00755 (SUB0123) comGF 125725..126180 (+) 456 WP_012657678.1 competence type IV pilus minor pilin ComGF -
  SUB_RS00760 (SUB0124) comGG 126158..126517 (+) 360 WP_012657679.1 competence type IV pilus minor pilin ComGG -
  SUB_RS00765 (SUB0125) comYH 126562..127518 (+) 957 WP_012657680.1 class I SAM-dependent methyltransferase Machinery gene
  SUB_RS00770 (SUB0126) - 127574..128764 (+) 1191 WP_012657681.1 acetate kinase -
  SUB_RS00775 (SUB0127) - 128882..129085 (+) 204 WP_012657682.1 helix-turn-helix transcriptional regulator -
  SUB_RS00780 (SUB0128) - 129087..129539 (+) 453 WP_012657683.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 40727.61 Da        Isoelectric Point: 9.7529

>NTDB_id=32905 SUB_RS00735 WP_172631729.1 123724..124779(+) (comYB) [Streptococcus uberis 0140J]
MINGIKRLSNFLNKDISITKKPSWKKLKAKQQYQLIKLLHNLSLSGFNLNEMVSFLEKAHLLENHFVQRMKIDLMNGRDI
SEMMAHLGFSDALVTQLSLAEVHGNTTNCLAKIEDYMAQSNRIRQKTIEVITYPMVLMTFLIVILMGLRHYLLPQIEEEN
QLTHFLNLFPTVLLLTFFAMIAVVIFLRLCWRKKSQLKQLLVYSKIPLLSAVLQLYLTAYYAREWGNLIAQGVELATILQ
LMQKEKSVLMREMGSTMEKALLEGKSFDQSVGIFPFFKRELSLMIEYGEIKGKLGQELDIYAQVSWENYFRKLRQMTQWL
QPLIFILVAIIIVMIYAAMLLPMYQTIGGEI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=32905 SUB_RS00735 WP_172631729.1 123724..124779(+) (comYB) [Streptococcus uberis 0140J]
ATAATCAATGGAATCAAGAGGTTGAGCAACTTCTTAAACAAGGATATATCGATAACGAAAAAGCCAAGTTGGAAAAAATT
AAAGGCTAAACAACAATACCAGCTGATCAAATTATTACATAATCTTAGTCTAAGTGGGTTTAACTTGAACGAAATGGTAT
CTTTTCTTGAGAAGGCACATCTCTTAGAGAATCATTTTGTTCAAAGAATGAAAATAGATTTAATGAATGGACGAGATATT
TCGGAAATGATGGCCCATCTTGGATTCTCAGATGCATTGGTAACACAGCTTAGTTTAGCTGAAGTCCATGGGAACACCAC
CAACTGTTTAGCAAAGATAGAAGATTATATGGCTCAATCCAATCGGATTAGACAAAAAACCATTGAAGTCATTACTTATC
CTATGGTCTTGATGACCTTCTTAATCGTGATTTTGATGGGCTTACGCCATTATTTACTTCCGCAAATAGAAGAGGAAAAC
CAGCTAACACATTTTCTAAATTTATTTCCGACAGTATTGCTTTTGACTTTCTTTGCTATGATTGCGGTAGTAATCTTCCT
CAGACTCTGCTGGCGAAAAAAATCCCAGTTAAAACAATTATTAGTGTATAGTAAAATCCCTCTTTTATCAGCTGTTTTAC
AATTGTATCTAACTGCCTATTATGCGAGAGAGTGGGGCAATCTTATTGCACAAGGTGTTGAACTAGCAACTATTTTGCAA
CTGATGCAAAAGGAAAAATCCGTATTGATGCGGGAAATGGGTTCAACAATGGAAAAAGCTTTGTTGGAAGGGAAATCTTT
TGACCAGTCTGTGGGCATCTTCCCATTTTTTAAAAGGGAATTGAGCTTAATGATTGAATATGGAGAAATCAAAGGAAAAC
TCGGTCAAGAGTTAGACATTTATGCACAAGTCAGTTGGGAAAACTATTTTAGAAAGTTACGACAGATGACTCAATGGTTA
CAACCCCTCATTTTCATACTTGTAGCCATTATAATCGTTATGATTTATGCGGCAATGTTATTGCCAATGTATCAAACCAT
CGGAGGTGAAATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(222-342)

Type II secretion system (T2SS), protein F (T2SSF)

(38-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA159

53.55

96.296

0.516
  comYB Streptococcus mutans UA140

53.55

96.296

0.516
  comYB Streptococcus gordonii str. Challis substr. CH1

47.337

96.296

0.456
  comGB/cglB Streptococcus mitis NCTC 12261

47.305

95.157

0.45
  comGB Lactococcus lactis subsp. cremoris KW2

44.767

98.006

0.439
  comGB/cglB Streptococcus mitis SK321

45.946

94.872

0.436
  comGB/cglB Streptococcus pneumoniae Rx1

44.745

94.872

0.425
  comGB/cglB Streptococcus pneumoniae D39

44.745

94.872

0.425
  comGB/cglB Streptococcus pneumoniae R6

44.745

94.872

0.425
  comGB/cglB Streptococcus pneumoniae TIGR4

44.745

94.872

0.425

Multiple sequence alignment