NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	IHMA87_RS24905	Genome accession	NZ_CP041354
Coordinates	5191928..5192395 (-)	Length	155 a.a.
NCBI ID	WP_034081326.1	Uniprot ID	Q8KQ32
Organism	Pseudomonas aeruginosa strain AZPAE15042
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5186928..5197395

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IHMA87_RS24875 (IHMA87_04861)	-	5187860..5188135 (+)	276	WP_012077290.1	head-tail connector protein	-
IHMA87_RS24880 (IHMA87_04862)	-	5188132..5188446 (+)	315	WP_034081329.1	phage head closure protein	-
IHMA87_RS24890	-	5188872..5189054 (+)	183	WP_123809553.1	hypothetical protein	-
IHMA87_RS24895 (IHMA87_04864)	tfpZ	5189174..5189926 (-)	753	WP_034081328.1	TfpX/TfpZ family type IV pilin accessory protein	-
IHMA87_RS24900 (IHMA87_04865)	-	5189923..5191863 (-)	1941	WP_034081327.1	hypothetical protein	-
IHMA87_RS24905 (IHMA87_04866)	pilA2	5191928..5192395 (-)	468	WP_034081326.1	pilin	Machinery gene
IHMA87_RS24910 (IHMA87_04867)	pilB	5192618..5194321 (+)	1704	WP_033998207.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IHMA87_RS24915 (IHMA87_04868)	pilC	5194325..5195542 (+)	1218	WP_034081324.1	type II secretion system F family protein	Machinery gene
IHMA87_RS24920 (IHMA87_04869)	pilD	5195545..5196417 (+)	873	WP_012077297.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
IHMA87_RS24925 (IHMA87_04870)	coaE	5196414..5197025 (+)	612	WP_003152767.1	dephospho-CoA kinase	-
IHMA87_RS24930 (IHMA87_04871)	yacG	5197022..5197222 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 155 a.a. Molecular weight: 15761.99 Da Isoelectric Point: 8.4401

                                >NTDB_id=325627 IHMA87_RS24905 WP_034081326.1 5191928..5192395(-) (pilA2) [Pseudomonas aeruginosa strain AZPAE15042]

MKAQKGFTLIELMIVVAIIGILAAVAIPAYQDYITRGQVTEAVSLGGGLKSPLAEYGADKNAWPTLVAPTATPGAGQLNA

TLVGKYSSVDSTIASGYPNGQITVTMTQGKASGKKLTFSTQDGGSSWACGNASIDGFAGTGTTIDAKYLPNACKP

Nucleotide

Download Length: 468 bp

                                >NTDB_id=325627 IHMA87_RS24905 WP_034081326.1 5191928..5192395(-) (pilA2) [Pseudomonas aeruginosa strain AZPAE15042]

ATGAAAGCGCAAAAAGGCTTTACCCTGATCGAACTAATGATCGTGGTGGCGATCATCGGCATTCTGGCTGCCGTCGCTAT

CCCGGCTTATCAGGACTACATCACCCGCGGTCAGGTAACCGAGGCCGTATCCCTGGGCGGTGGTCTGAAGTCCCCGCTGG

CCGAGTATGGCGCCGACAAGAACGCTTGGCCGACTCTGGTTGCCCCAACTGCTACTCCTGGGGCTGGTCAATTGAATGCA

ACTCTGGTTGGCAAGTATTCGAGCGTAGATAGCACCATTGCCAGCGGCTATCCTAACGGTCAGATCACTGTCACCATGAC

TCAAGGTAAGGCTTCTGGTAAGAAGCTGACCTTCAGCACTCAAGACGGGGGTTCGTCTTGGGCGTGTGGTAATGCTTCTA

TCGACGGTTTTGCCGGTACCGGTACCACGATCGACGCTAAGTACCTGCCGAACGCTTGCAAGCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-154)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q8KQ32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	38.743	100	0.477
pilA2	Legionella pneumophila str. Paris	41.875	100	0.432
pilA2	Legionella pneumophila strain ERS1305867	41.875	100	0.432
pilE	Neisseria gonorrhoeae MS11	41.104	100	0.432
pilE	Neisseria gonorrhoeae strain FA1090	41.509	100	0.426
pilA	Ralstonia pseudosolanacearum GMI1000	38.462	100	0.419
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.024	100	0.413
comP	Acinetobacter baylyi ADP1	39.375	100	0.406
pilA	Pseudomonas aeruginosa PAK	36.257	100	0.4
pilA/pilA1	Eikenella corrodens VA1	37.107	100	0.381
pilA	Vibrio parahaemolyticus RIMD 2210633	43.182	85.161	0.368

Detailed information NTG0325627