Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EBN15_RS14145 Genome accession   NZ_CP033326
Coordinates   3272089..3273342 (+) Length   417 a.a.
NCBI ID   WP_159407405.1    Uniprot ID   -
Organism   Xanthomonas cucurbitae strain ATCC 23378     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3267089..3278342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBN15_RS14130 (EBN15_14190) pilR 3267254..3268645 (+) 1392 WP_167526932.1 sigma-54 dependent transcriptional regulator Regulator
  EBN15_RS14135 (EBN15_14195) pilB 3269466..3271202 (-) 1737 WP_159407403.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EBN15_RS14140 (EBN15_14200) pilA/pilAI 3271328..3271735 (-) 408 WP_159407404.1 pilin Machinery gene
  EBN15_RS14145 (EBN15_14205) pilC 3272089..3273342 (+) 1254 WP_159407405.1 type II secretion system F family protein Machinery gene
  EBN15_RS14150 (EBN15_14210) - 3273349..3274212 (+) 864 WP_159407406.1 A24 family peptidase -
  EBN15_RS14155 (EBN15_14215) coaE 3274226..3274831 (+) 606 WP_104604291.1 dephospho-CoA kinase -
  EBN15_RS14160 (EBN15_14220) - 3275046..3276380 (-) 1335 WP_159407407.1 HAMP domain-containing sensor histidine kinase -
  EBN15_RS14165 (EBN15_14225) - 3276836..3277513 (-) 678 WP_003490678.1 response regulator transcription factor -
  EBN15_RS14170 (EBN15_14230) - 3277576..3277932 (-) 357 WP_159407408.1 hypothetical protein -

Sequence

Protein


Download         Length: 417 a.a.        Molecular weight: 45422.55 Da        Isoelectric Point: 10.2578

>NTDB_id=322979 EBN15_RS14145 WP_159407405.1 3272089..3273342(+) (pilC) [Xanthomonas cucurbitae strain ATCC 23378]
MSTVRNTSKLRSAPSSQLSPFVWEGKDKRGTTMRGEQAARSANLLRAELRKQGITPTVVKPKPKPLFGAAGKKVTAKDIA
FFSRQMATMMKSGVPIVGALEIIGEGQKNPRMRTMVGQIRTDIEGGSSLYEAISKHPVQFDELYRNLVRAGEGAGVLETV
LDTIANYKENIEALKGKIRKALFYPAMVMAVALLVSSILLIWVVPQFEDVFKGFGAELPAFTQMIVAASRFMVSWWWALL
LVTIGAVTGMIFAYKRSPAMQHKLDRLVLKVPVIGQIMHNSSVARFARTTAVTFKAGVPLVEALSIVAGATGNSVYEEAV
LRMRDDVSVGYPVNVAMKQVSLFPHMVVQMTAIGEEAGALDSMLFKVAEYFEQEVNNAVDALSSLLEPLIMVFIGTIVGG
MVIGMYLPIFKLASVVG

Nucleotide


Download         Length: 1254 bp        

>NTDB_id=322979 EBN15_RS14145 WP_159407405.1 3272089..3273342(+) (pilC) [Xanthomonas cucurbitae strain ATCC 23378]
ATGTCAACTGTACGCAACACCTCGAAGTTAAGATCAGCGCCTTCATCTCAGTTGAGCCCGTTTGTCTGGGAGGGAAAAGA
CAAGCGCGGCACAACGATGAGGGGGGAACAAGCCGCGCGCAGCGCAAATCTTCTGCGAGCCGAATTGCGCAAGCAGGGAA
TTACTCCCACCGTGGTAAAACCTAAGCCCAAGCCGCTGTTTGGCGCGGCCGGCAAGAAGGTTACCGCTAAAGACATCGCC
TTTTTTAGTAGACAGATGGCGACAATGATGAAATCTGGCGTGCCAATCGTTGGCGCCCTTGAAATTATCGGTGAGGGGCA
AAAGAATCCCCGCATGCGCACTATGGTAGGCCAGATCCGCACCGATATTGAGGGTGGATCATCGCTGTACGAAGCAATCA
GCAAGCATCCTGTGCAGTTCGATGAGCTTTACCGAAACCTGGTTCGCGCTGGCGAAGGTGCAGGCGTACTTGAGACGGTG
CTAGACACCATCGCCAATTACAAAGAGAACATTGAGGCACTCAAGGGCAAAATCAGAAAGGCGCTCTTCTACCCAGCTAT
GGTGATGGCCGTAGCCCTTTTGGTTAGCTCAATCCTCCTGATCTGGGTAGTCCCGCAATTTGAGGACGTCTTCAAAGGTT
TCGGCGCCGAGCTACCGGCTTTCACTCAAATGATCGTAGCAGCATCGAGATTCATGGTGTCATGGTGGTGGGCGCTATTG
CTTGTAACGATTGGCGCAGTCACGGGAATGATATTTGCCTACAAGCGCTCCCCAGCGATGCAGCATAAGCTTGATCGGCT
CGTGCTAAAGGTGCCAGTGATTGGGCAGATCATGCACAACAGCTCGGTCGCGAGATTTGCTCGAACAACCGCAGTTACTT
TCAAGGCAGGCGTCCCGCTTGTCGAGGCATTGAGCATAGTCGCGGGCGCCACCGGAAACTCAGTATACGAGGAAGCAGTA
CTTCGCATGCGAGACGACGTTTCGGTCGGATACCCGGTCAACGTAGCCATGAAGCAAGTCAGTCTCTTTCCACACATGGT
TGTTCAGATGACAGCGATCGGTGAGGAAGCTGGTGCGCTCGACTCCATGCTCTTCAAGGTCGCCGAGTATTTCGAGCAAG
AGGTCAATAACGCAGTAGACGCTTTAAGCAGCCTTTTAGAACCCCTGATCATGGTGTTCATTGGTACCATCGTGGGCGGC
ATGGTCATCGGCATGTACCTGCCCATCTTCAAGCTCGCTTCGGTGGTTGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(286-408)

Type II secretion system (T2SS), protein F (T2SSF)

(82-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.015

95.444

0.506
  pilC Legionella pneumophila strain ERS1305867

51.99

96.403

0.501
  pilC Acinetobacter baylyi ADP1

50.614

97.602

0.494
  pilC Acinetobacter baumannii D1279779

49.132

96.643

0.475
  pilG Neisseria gonorrhoeae MS11

42.75

95.923

0.41
  pilG Neisseria meningitidis 44/76-A

42.25

95.923

0.405
  pilC Vibrio cholerae strain A1552

40.441

97.842

0.396
  pilC Thermus thermophilus HB27

39.461

97.842

0.386
  pilC Vibrio campbellii strain DS40M4

39.702

96.643

0.384

Multiple sequence alignment