Detailed information NTG0322977

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: EBN15_RS14135
• Genome accession: NZ_CP033326
• Coordinates: 3269466..3271202 (-)
• Length: 578 a.a.
• NCBI ID: WP_159407403.1
• Uniprot ID: -
• Organism: Xanthomonas cucurbitae strain ATCC 23378
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3264466..3276202

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EBN15_RS14125 (EBN15_14185)	-	3265481..3267094 (+)	1614	WP_104604280.1	HAMP domain-containing sensor histidine kinase	-
EBN15_RS14130 (EBN15_14190)	pilR	3267254..3268645 (+)	1392	WP_167526932.1	sigma-54 dependent transcriptional regulator	Regulator
EBN15_RS14135 (EBN15_14195)	pilB	3269466..3271202 (-)	1737	WP_159407403.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EBN15_RS14140 (EBN15_14200)	pilA/pilAI	3271328..3271735 (-)	408	WP_159407404.1	pilin	Machinery gene
EBN15_RS14145 (EBN15_14205)	pilC	3272089..3273342 (+)	1254	WP_159407405.1	type II secretion system F family protein	Machinery gene
EBN15_RS14150 (EBN15_14210)	-	3273349..3274212 (+)	864	WP_159407406.1	A24 family peptidase	-
EBN15_RS14155 (EBN15_14215)	coaE	3274226..3274831 (+)	606	WP_104604291.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62727.97 Da
• Isoelectric Point: 5.9537

>NTDB_id=322977 EBN15_RS14135 WP_159407403.1 3269466..3271202(-) (pilB) [Xanthomonas cucurbitae strain ATCC 23378]
MSVAGAANLVGITGIARRLVQDGALEELAARTAMEQAAAAKVPLPQWFVEKKLVSAAQLAAANAVEFGMPLMDVSVFDAS
QNAIKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
GDDEEMGDLDVSAGDEDMGAGGDAGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKTSLP
EHALLAEGFTQQQIDQGITLYEAVGCDDCTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=322977 EBN15_RS14135 WP_159407403.1 3269466..3271202(-) (pilB) [Xanthomonas cucurbitae strain ATCC 23378]
ATGAGTGTTGCTGGAGCAGCCAATTTGGTTGGCATCACAGGGATTGCTCGCCGACTAGTTCAGGATGGCGCGCTGGAGGA
GTTGGCTGCACGTACCGCCATGGAACAAGCCGCTGCGGCTAAGGTACCTTTGCCGCAGTGGTTCGTCGAAAAGAAGCTTG
TGTCAGCGGCACAGTTGGCGGCGGCAAACGCAGTCGAGTTCGGCATGCCACTGATGGACGTATCGGTGTTCGACGCCAGC
CAGAACGCGATCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGGAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTTGATGACATCAAGTTTCATACGAACCTGGTGGTCGAGCCGA
TCCTGGTGGACGAGGACCAGATCCGTCGCACCTTGGAGCAATGGCAAGCTAGCAACGCATCGCTTGGTTCATCGCTGGGG
GGCGATGACGAAGAGATGGGCGACCTGGACGTCTCGGCCGGCGACGAAGACATGGGCGCCGGCGGGGATGCCGGGGTCGA
TGCCAAGGGTGATGACACGCCGGTGGTGAAATTCGTCAACAAGGTGTTGGTGGATGCGATCCGGCGGGGAGCCTCCGATA
TCCATTTCGAGCCTTATGAAGACGACTACCGTGTTCGCTTGCGCATCGATGGGCTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGGATTGCGGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
TGGGCGCATCAAGCTTAATCTGTCCAAGACCAAACAGATCGACTTCCGTGTGAGTACCTTGCCGACGCTGTTCGGCGAGA
AGGTGGTGCTACGTATCCTGGATGGCAGTGCGGCCAAGCTGGGGATCGAGAAGCTAGGTTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCCTT
GTATACGGCGCTGGGTATCCTCAACGATGAGACGCGCAACATCTCTACTGCCGAGGATCCGGTTGAAATTCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCGGCGGCCTTGCGCTCGTTCCTGCGCCAGGACCCT
GACATCATCATGGTCGGCGAAATTCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCATATGGT
GCTGTCCACGTTGCATACCAACGATGCGCCACAGACCATCGCGCGCTTGATGAACATGGGGATTGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTCATTGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGACCAGCTTGCCA
GAGCATGCGTTGCTGGCCGAAGGGTTTACGCAACAGCAGATCGACCAGGGCATTACGCTGTATGAGGCGGTCGGTTGCGA
TGACTGCACCGAGGGCTACAAGGGCCGTACGGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGTGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCGGCGCAGAAGATCGGTATCCGCGACTTGCGGCAGTCGGCCTTGATG
AAGGCCGCGCATGGGGTGACCAGCCTGGCGGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-153)

• Type II/IV secretion system protein (T2SSE) (201-468)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.162	98.27	0.552
pilB	Acinetobacter baylyi ADP1	55.458	98.27	0.545
pilB	Legionella pneumophila strain ERS1305867	52.373	98.443	0.516
pilB	Vibrio cholerae strain A1552	49.04	99.135	0.486
pilF	Neisseria gonorrhoeae MS11	48.592	98.27	0.478
pilB	Vibrio campbellii strain DS40M4	46.127	98.27	0.453
pilB	Vibrio parahaemolyticus RIMD 2210633	45.922	97.578	0.448
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.002	93.599	0.365