Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SBAL223_RS06855 Genome accession   NC_011663
Coordinates   1572245..1573357 (+) Length   370 a.a.
NCBI ID   WP_006082516.1    Uniprot ID   A0A9X3AYD3
Organism   Shewanella baltica OS223     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1567245..1578357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBAL223_RS06825 (Sbal223_1334) - 1567968..1568402 (-) 435 WP_006082522.1 DUF4426 domain-containing protein -
  SBAL223_RS06830 (Sbal223_1335) yggU 1568534..1568833 (-) 300 WP_012587167.1 DUF167 family protein YggU -
  SBAL223_RS06835 (Sbal223_1336) - 1568833..1569381 (-) 549 WP_006082520.1 YggT family protein -
  SBAL223_RS06840 (Sbal223_1337) proC 1569430..1570248 (-) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  SBAL223_RS06845 (Sbal223_1338) - 1570464..1571162 (-) 699 WP_041408417.1 YggS family pyridoxal phosphate-dependent enzyme -
  SBAL223_RS06850 (Sbal223_1339) pilT 1571198..1572235 (+) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  SBAL223_RS06855 (Sbal223_1340) pilU 1572245..1573357 (+) 1113 WP_006082516.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SBAL223_RS06860 (Sbal223_1341) - 1573544..1574083 (+) 540 WP_012587169.1 glutathione peroxidase -
  SBAL223_RS06865 (Sbal223_1342) hemH 1574116..1575129 (+) 1014 WP_012587170.1 ferrochelatase -
  SBAL223_RS06870 (Sbal223_1343) ruvX 1575215..1575640 (-) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  SBAL223_RS06875 (Sbal223_1344) - 1575670..1576233 (-) 564 WP_006082512.1 YqgE/AlgH family protein -
  SBAL223_RS06880 (Sbal223_1345) yciH 1576300..1576629 (-) 330 WP_037392612.1 stress response translation initiation inhibitor YciH -
  SBAL223_RS06885 (Sbal223_1346) - 1576808..1577125 (-) 318 WP_012587171.1 hypothetical protein -
  SBAL223_RS06890 (Sbal223_1347) - 1577304..1577864 (+) 561 WP_006082509.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40962.07 Da        Isoelectric Point: 6.2352

>NTDB_id=32031 SBAL223_RS06855 WP_006082516.1 1572245..1573357(+) (pilU) [Shewanella baltica OS223]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSSRECNFAFAAKDLGR
FRVSAFWQRESSGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=32031 SBAL223_RS06855 WP_006082516.1 1572245..1573357(+) (pilU) [Shewanella baltica OS223]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCTGACTTGTTCATCACAGCTGGCTTTCCGCCAAG
TGCCAAGATTGACGGTGAATTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCACTGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCGAGTCGTGAGTGTAACTTTGCTTTTGCGGCAAAGGATTTAGGTCGT
TTTCGGGTGAGCGCCTTCTGGCAGCGTGAATCGTCAGGCTGCGTGATGCGCCGTATCGAAACTAAAATCCCTGACGTCGA
TGATTTAAAACTGCCACCTATTCTGAAAGATCTGGTGATGAGTAAACGTGGCCTCATCATTATGGTGGGCGGAACGGGTA
CGGGTAAGTCAACATCTCTGGCGGCCTTGGTGGGATATCGTAATGGTCATGCTCGTGGTCATATTCTCACGATTGAAGAT
CCGGTCGAATTCGTTCACGACCACAGAAAAAGCATTATCACCCAGCGTGAAGTGGGCATAGATACTGAGTCTTTTGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCACCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CCCTGTCGTTTGCGGAAACGGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAACAGTTATTGTTCGATTTGTCATTGAATTTACGCGGGATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGCACTGGGCGTCGCGCCGCGATTGAAGTATTGATCAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTGCTCAAAGAAACCATGGCCAAATCGAATGAGCAGGGCATGCAGACGTTCGATCAAGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCGAACGATTTACGTTT
GATGATTAAATTGCAGAATAAAGAATCCACGGGCTCAGGTTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment