Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DV532_RS23510 Genome accession   NZ_CP032677
Coordinates   5079590..5080858 (-) Length   422 a.a.
NCBI ID   WP_082476757.1    Uniprot ID   -
Organism   Pseudomonas sp. Leaf58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5074590..5085858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV532_RS23495 (DV532_23495) - 5076234..5077856 (-) 1623 WP_056794378.1 SPOR domain-containing protein -
  DV532_RS23500 (DV532_23500) aroB 5077872..5078969 (-) 1098 WP_056794377.1 3-dehydroquinate synthase -
  DV532_RS23505 (DV532_23505) aroK 5079067..5079585 (-) 519 WP_056794375.1 shikimate kinase AroK -
  DV532_RS23510 (DV532_23510) pilQ 5079590..5080858 (-) 1269 WP_082476757.1 type IV pilus secretin PilQ Machinery gene
  DV532_RS23515 (DV532_23515) - 5080782..5081798 (-) 1017 WP_056794370.1 pilus assembly protein PilP -
  DV532_RS23520 (DV532_23520) - 5081795..5082331 (-) 537 WP_056794368.1 PilN domain-containing protein -
  DV532_RS23525 (DV532_23525) pilM 5082328..5083200 (-) 873 WP_056794367.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45769.38 Da        Isoelectric Point: 4.7577

>NTDB_id=318121 DV532_RS23510 WP_082476757.1 5079590..5080858(-) (pilQ) [Pseudomonas sp. Leaf58]
MKTVMKWGLAPLLSMQLASAAPYQGEPLSLNFQDVEVRSVLQVLADYTGVNLVASDAVQGNVTLRLQDVPWDQALDLVLR
SKGLAQRQEGNVLLVAPAAELAAQSFDTRVGQVLDAQLQPLQRELVAIHHAKAAELAELLMASLADDGILTGRGSLSVDE
RTNTLVVYQPVERLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYAESAQLGKDVFVDLGVERAGSSI
GLGLLRGGVLLDLELSAMEKSGNGEIISQPKVVTANKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=318121 DV532_RS23510 WP_082476757.1 5079590..5080858(-) (pilQ) [Pseudomonas sp. Leaf58]
ATGAAAACAGTGATGAAGTGGGGCCTGGCGCCCCTGCTGTCGATGCAATTGGCGTCGGCGGCGCCTTATCAGGGTGAGCC
ACTGTCGTTGAACTTTCAGGATGTGGAGGTGCGTTCGGTGTTGCAGGTGCTGGCCGACTATACCGGCGTCAATTTGGTTG
CTAGCGACGCCGTGCAGGGCAATGTCACCTTGCGTTTGCAGGATGTGCCGTGGGACCAGGCCCTTGACCTGGTGTTGCGC
AGCAAGGGCCTGGCCCAGCGTCAGGAAGGCAATGTGTTGCTGGTGGCGCCTGCGGCCGAGCTTGCCGCACAATCGTTCGA
TACCCGCGTAGGGCAGGTGCTGGATGCGCAGTTGCAACCGTTGCAACGGGAGCTGGTGGCCATCCACCACGCCAAGGCCG
CCGAGTTGGCGGAGTTACTGATGGCAAGCCTGGCGGATGACGGCATTCTTACTGGGCGGGGTAGCCTGAGTGTCGATGAG
CGCACCAATACCTTGGTGGTGTACCAGCCTGTCGAGCGCCTTGCCGAGCTGCGGCAATTGGTGGCGCAGCTTGATGTACC
GGTACGCCAGGTGGCGATCGAGGCGCGCATCGTCGAAGCCAATGTCGATTACGAAAAGAGCCTGGGGGTACGCTGGGGTG
GGCCTCTGTACGCTGAAAGCGCACAACTGGGCAAGGACGTGTTCGTCGACCTCGGGGTGGAGCGGGCTGGCAGCAGCATC
GGCCTGGGCTTGCTGCGCGGCGGGGTGTTACTTGACCTTGAGCTCAGCGCCATGGAAAAGAGCGGCAACGGCGAGATCAT
CTCGCAACCCAAGGTGGTCACGGCTAACAAGGAAACGGCCAGGATACTCAAGGGCACCGAGGTGCCGTACCAGGAAACCA
GCCAGAGTGGCGCTACCTCGGTGTCGTTCCGCGAGGCCTCGTTGTCGCTGGAGGTGACCCCGCAAATCACCCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGACTACGTCAATGCCTTGAACAACGTACCGCCGATTCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGCTGACGGCGAAACGATCGTTATCGGTGGCGTCTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

60.78

100

0.628
  comQ Acinetobacter baylyi ADP1

42.316

100

0.45
  pilQ Acinetobacter baumannii D1279779

40.611

100

0.441
  pilQ Acinetobacter baumannii strain A118

40.611

100

0.441
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.468

99.763

0.434
  pilQ Vibrio cholerae strain A1552

43.468

99.763

0.434
  pilQ Vibrio campbellii strain DS40M4

41.745

100

0.419
  pilQ Legionella pneumophila strain ERS1305867

42.344

99.052

0.419
  pilQ Legionella pneumophila strain Lp02

42.344

99.052

0.419
  pilQ Neisseria gonorrhoeae strain FA1090

37.56

99.052

0.372
  pilQ Neisseria meningitidis M1080

36.364

99.052

0.36
  pilQ Neisseria meningitidis MC58

36.364

99.052

0.36

Multiple sequence alignment